vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2023-13-2-310-317URUPYYvuzbiochemi-1025Research ArticleКРАТКИЕ СООБЩЕНИЯBRIEF COMMUNICATIONОтбор травянистого целлюлозосодержащего сырья, пригодного для биотехнологической переработкиSelection of herbaceous cellulose-containing raw materials for biotechnological processinghttps://orcid.org/0000-0002-6567-9662ГладышеваЕ. К.GladyshevaE. K.

Гладышева Евгения Константиновна - кандидат технических наук, научный сотрудник лаборатории биоконверсии.

659322, Бийск, ул. Социалистическая, 1

Evgeniya K. Gladysheva - Cand. Sci. (Engineering), Researcher, Laboratory of Bioconversion.

1, Sotsialisticheskaya St., Biysk, 659322

evg-gladysheva@yandex.ruhttps://orcid.org/0000-0002-1628-0815БудаеваВ. В.BudaevaV. V.

Будаева Вера Владимировна - кандидат химических наук, доцент, заведующая лабораторией биоконверсии.

659322, Бийск, ул. Социалистическая, 1

Vera V. Budaeva - Cand. Sci (Chemistry), Associated Professor, Head of the Laboratory of Bioconversion.

1, Sotsialisticheskaya St., Biysk, 659322

budaeva@ipcet.ruhttps://orcid.org/0000-0001-8897-347XСкибаЕ. А.SkibaE. A.

Скиба Екатерина Анатольевна - кандидат технических наук, доцент, старший научный сотрудник лаборатории биоконверсии.

659322, Бийск, ул. Социалистическая, 1

Ekaterina А. Skiba - Cand. Sci. (Engineering), Associated Professor, Senior Researcher, Laboratory of Bioconversion.

1, Sotsialisticheskaya St., Biysk, 659322

eas08988@mail.ruhttps://orcid.org/0000-0003-1593-7982КащееваЕ. И.KashcheevaE. I.

Кащеева Екатерина Ивановна - кандидат технических наук, старший научный сотрудник лаборатории биоконверсии.

659322, Бийск, ул. Социалистическая, 1

Ekaterina I. Kashcheeva - Cand. Sci. (Engineering), Senior Researcher, Laboratory of Bioconversion.

1, Sotsialisticheskaya St., Biysk, 659322

massl@mail.ruhttps://orcid.org/0000-0002-9630-6332ЗолотухинВ. Н.ZolotuhinV. N.

Золотухин Владимир Николаевич - кандидат технических наук, старший научный сотрудник лаборатории биоконверсии.

659322, Бийск, ул. Социалистическая, 1

Vladimir N. Zolotuhin - Cand. Sci. (Engineering), Senior Researcher, Laboratory of Bioconversion.

1, Sotsialisticheskaya St., Biysk, 659322

ipcet@mail.ruИнститут проблем химико-энергетических технологий СО РАНРоссияInstitute for Problems of Chemical and Energetic Technologies of the SB RASRussian Federation202302072023132310317Copyright © Гладышева Е.К., Будаева В.В., Скиба Е.А., Кащеева Е.И., Золотухин В.Н., 20232023Гладышева Е.К., Будаева В.В., Скиба Е.А., Кащеева Е.И., Золотухин В.Н.Gladysheva E.K., Budaeva V.V., Skiba E.A., Kashcheeva E.I., Zolotuhin V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1025

Использование целлюлозосодержащего растительного сырья для производства биопродуктов является одним из ключевых вопросов устойчивого экономического развития. Травянистое целлюлозосодержащее сырье – наиболее распространенный и легковозобновляемый ресурс. Данная работа посвящена отбору травянистого целлюлозосодержащего сырья, пригодного для биотехнологической переработки, исследованы следующие источники: тростник, мискантус сорта Сорановский, водяной гиацинт, салат Айсберг, суданская трава, шелуха овса, солома льна-межеумка. Предварительная химическая обработка сырья проведена классическим методом щелочной делигнификации при атмосферном давлении, полученные субстраты превращены в раствор редуцирующих сахаров методом ферментативного гидролиза. Установлено, что методом щелочной делигнификации исходного сырья возможно получить продукты с массовым содержанием целлюлозы по Кюршнеру от 82,9 до 93,1%, что является хорошим показателем для дальнейшего ферментативного гидролиза. По результатам ферментативного гидролиза продуктов щелочной делигнификации выявлено, что наибольшую реакционную способность к ферментативному гидролизу имели продукты щелочной делигнификации мискантуса сорта Сорановский, салата Айсберг и шелухи овса: концентрация редуцирующих веществ составила 25,0; 28,4 и 26,9 г/л (выход редуцирующих веществ от массы субстрата составил 75,0; 85,2 и 80,7%) соответственно. Таким образом, высокая реакционная способность этих видов сырья позволяет рекомендовать их для дальнейшей биотехнологической переработки. Для других видов сырья необходима оптимизация стадии щелочной делигнификации для повышения реакционной способности к ферментативному гидролизу.

The use of cellulose-containing plant materials for obtaining bioproducts comprises a relevant research direction in the field of sustainable economic development. Herbaceous cellulose-containing raw materials are among the most widespread and easily renewable resources. In this study, we set out to identify herbaceous cellulose-containing raw materials suitable for biotechnological processing among the following plants: cane, miscanthus (Soranovsky variety), water hyacinth, iceberg lettuce, Sudan grass, oat husk, flax straw (Linum usitatissimum L.). Preliminary chemical treatment of raw materials was carried out by the conventional method of alkaline delignification at atmospheric pressure. The obtained substrates were converted into a solution of reducing sugars by enzymatic hydrolysis. The method of alkaline delignification of initial raw materials was found to be suitable for obtaining products with the cellulose mass content of 82.9–93.1% by the Kurschner method. This conversion rate can be considered a good indicator for further enzymatic hydrolysis. According to the results of enzymatic

hydrolysis of alkaline delignification products, the highest reactivity to enzymatic hydrolysis was demonstrated by the alkaline delignification products of miscanthus (Soranovsky variety), iceberg lettuce and oat husk. For these plants, the concentration of reducing substances reached 25.0, 28.4 and 26.9 g/l, under the yield of reducing substances from the substrate mass of 75.0, 85.2 and 80.7%, respectively. Therefore, the high reactivity of these plant materials makes them prospective candidates for further biotechnological processing. Other investigated plant materials require optimization of the alkaline delignification stage to increase their reactivity to enzymatic hydrolysis.

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The authors declare that there are no conflicts of interest present.