vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2023-13-3-434-441PQNFQNvuzbiochemi-1078Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYПовышение устойчивости культуры клеток Arabidopsis thaliana к фториду натрия за счет конститутивной экспрессии HSP101Increased Arabidopsis thaliana cell culture resistance to sodium fluoride by constitutive expression of HSP101https://orcid.org/0000-0002-6858-380XГорбылеваЕ. Л.GorbylevaE. L.

Горбылева Елена Леонидовна - кандидат биологических наук, младший научный сотрудник.

664033, Иркутск, ул. Лермонтова, 132

Elena L. Gorbyleva - Cand. Sci. (Biology), Researcher.

132, Lermontov St., 664033, Irkutsk

dzubina@sifibr.irk.ruhttps://orcid.org/0000-0001-9495-6246СафоноваМ. А.SafonovaM. A.

Сафонова Мария Александровна - кандидат биологических наук, преподаватель.

664011, Иркутск, ул. Карла Маркса, 37

Mariya A. Safonova - Cand. Sci. (Biology), lecturer.

37, K. Marx St., 664011, Irkutsk

marjera@mail.ruhttps://orcid.org/0000-0002-0456-3690СтепановА. В.StepanovA. V.

Степанов Алексей Владимирович - кандидат биологических наук, старший научный сотрудник.

664033, Иркутск, ул. Лермонтова, 132

Alexey V. Stepanov - Cand. Sci. (Biology), Senior Researcher.

132, Lermontov St., 664033, Irkutsk

stepanov@sifibr.irk.ruhttps://orcid.org/0000-0001-7758-4540РихвановЕ. Г.RikhvanovE. G.

Рихванов Евгений Геннадьевич - доктор биологических наук, старший научный сотрудник.

664033, Иркутск, ул. Лермонтова, 132

Evgenii G. Rikhvanov - Cand. Sci. (Biology), Senior Researcher.

132, Lermontov St., 664033, Irkutsk

eugene@sifibr.irk.ruСибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and BiochemistryRussian FederationБайкальский гуманитарный институтРоссияBaikal Humanitarian InstituteRussian Federation202330092023133434441Copyright © Горбылева Е.Л., Сафонова М.А., Степанов А.В., Рихванов Е.Г., 20232023Горбылева Е.Л., Сафонова М.А., Степанов А.В., Рихванов Е.Г.Gorbyleva E.L., Safonova M.A., Stepanov A.V., Rikhvanov E.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1078

Фтор является одним из токсических компонентов выбросов промышленных предприятий. Повышенное содержание фтора в атмосфере, почве или воде негативно влияет на рост и развитие растений, а также снижает устойчивость к различным стрессовым факторам окружающей среды. Увеличение температуры окружающей среды вызывает у всех организмов защитный ответ в виде синтеза белков теплового шока. У растений имеется специфический белок НSP101, который выполняет функцию защиты клеток от тепловых повреждений и также является ответственным за индуцируемую термотолерантность – способность организмов выдерживать воздействие жесткого теплового шока, будучи предварительно подвергнутыми действию мягкого теплового стресса, в результате которого индуцируются белки теплового шока. Известно, что белки теплового шока участвуют в защите не только от повышенных температур, но и от других различных стрессовых факторов. В настоящей работе было изучено влияние обработки фторидом натрия на жизнеспособность культуры клеток Arabidopsis thaliana, экспрессию и синтез белков теплового шока, а также роль белка теплового шока HSP101 в устойчивости к фтору. Показано, что фторид натрия значительно снижает жизнеспособность клеток A. thaliana, подавляет активацию экспрессии гена HSP101 при повышении температуры. В то же время линия A. thaliana с конститутивной экспрессией гена HSP101 оказалась более устойчива к токсичному действию фторида натрия.

Fluorine is one of the toxic elemental components of industrial emissions. Increased fluoride content in the atmosphere, soil or water negatively affects the growth and development of plants, as well as reducing resistance to various environmental stressors. An increase in ambient temperature causes a protective response in all organisms taking the form heat shock protein synthesis. The specific protein HSP101, which performs the function of protecting plant cells from heat damage, is also responsible for inducible thermotolerance, representing the ability of organisms to withstand the effects of severe heat shock that were previously exposed to mild heat stress, as a result of which heat shock proteins are induced. Heat shock proteins are involved in protecting not only against elevated temperatures, but also various other stress factors. In this work, the effect of sodium fluoride treatment on the viability of Arabidopsis thaliana cell culture, expression and synthesis of heat shock proteins was studied along with the role of heat shock protein HSP101 in providing resistance to fluoride. Sodium fluoride has been shown to significantly reduce the viability of A. thaliana cells by suppressing the activation of HSP101 gene expression with an increase in temperature. At the same time, the A. thaliana line, which has constitutive expression of the HSP101 gene, proved to be more resistant to the toxic effects of sodium fluoride.

фторид натрияArabidopsis thalianaбелки теплового шокакультура клетоктепловой стрессsodium fluorideArabidopsis thalianaheat shock proteinscell cultureheat stressАвторы выражают благодарность ведущему инженеру Т.М. Русалевой за помощь в выделении белка и проведении иммуноблоттинга с антителами, а также профессору Е. Виерлингу и доктору М. Эскобару (Университет Аризоны, США) за предоставленные антитела к Hsp101 и Hsp17.6 (класс I и II). Работа выполнена с использованием коллекции Центра коллективного пользования (ЦКП) «Биоресурсный центр» и оборудования ЦКП «Биоаналитика» Сибирского института физиологии и биохимии растений СО РАН (г. Иркутск)The authors are grateful leading ingeneer T.M. Rusaleva for the help in isolation protein fractions and immunoblotting with antibodies, and also to Dr. M. Escobar and Professor E. Vierling (University of Arizona, USA) for the gift of antibodies to Hsp101 and Hsp17.6 (class I and II). 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The authors declare that there are no conflicts of interest present.