vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2023-13-4-494-505LJBWLSvuzbiochemi-1119Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYМодуляция роста и аккумуляции химических элементов в растениях Fragaria × ananassa в условиях in vivo под действием хелатов кремнияModulation of growth and chemical element accumulation in Fragaria × ananassa plants in vivo under the effect of silicon chelateshttps://orcid.org/0000-0002-2119-6503АмбросЕ. В.AmbrosE. V.

Амброс Елена Валерьевна, к.б.н., старший научный сотрудник

630090, г. Новосибирск, ул. Золотодолинская, 101

Elena V. Ambros, Cand. Sci. (Biology), Senior Researcher

101, Zolotodolinskaya St., Novosibirsk, 630090

ambros_ev@mail.ruhttps://orcid.org/0000-0003-4064-9106КруповичЕ. С.KrupovichE. S.

Крупович Елена Сергеевна, инженер-исследователь

630090, г. Новосибирск, ул. Золотодолинская, 101

Elena S. Krupovich, Engineer-Researcher

101, Zolotodolinskaya St., Novosibirsk, 630090

e.krupovich@g.nsu.ruhttps://orcid.org/0009-0004-1763-5456КолмогоровЮ. П.KolmogorovYu. P.

Колмогоров Юрий Петрович, ведущий электроник

630090, г. Новосибирск, пр. Академика Коптюга, 3

Yurii P. Kolmogorov, Leading Electronics Engineer

3, Akademik Koptyug Ave., Novosibirsk, 630090

kolmogorov@igm.nsc.ruhttps://orcid.org/0000-0002-6769-3724ТрофимоваЕ. Г.TrofimovaE. G.

Трофимова Елена Геннадиевна, научный сотрудник

630128, г. Новосибирск, ул. Кутателадзе, 18

Elena G. Trofimova, Researcher

18, Kutateladze St., Novosibirsk, 630128

shapolovaelena@yandex.ruhttps://orcid.org/0000-0002-1088-2700ГусевИ. С.GusevI. S.

Гусев Иван Сергеевич, инженер-исследователь

630090, г. Новосибирск, пр. Академика Лаврентьева, 11

Ivan S. Gusev, Engineer-Researcher

11, Akademik Lavrentiev Ave., Novosibirsk, 30090

gusev@yandex.ruhttps://orcid.org/0000-0002-9605-784XГольденбергБ. Г.GoldenbergB. G.

Гольденберг Борис Григорьевич, к.т.н., старший научный сотрудник

630090, г. Новосибирск, пр. Академика Лаврентьева, 11

 

Boris G. Goldenberg, Cand. Sci. (Engineering), Senior Researcher

11, Akademik Lavrentiev Ave., Novosibirsk, 30090

1, Nikolsky Ave., Koltsovo, 630559, Novosibirsk region

b.g.goldenberg@srf-skif.ruЦентральный Сибирский ботанический сад СО РАНРоссияCentral Siberian Botanical Garden SB RASRussian FederationИнститут геологии и минералогии им. В.С. Соболева СО РАНРоссияV.S. Sobolev Institute of Geology and Mineralogy SB RASRussian FederationИнститут химии твердого тела и механохимии СО РАНРоссияInstitute of Solid State Chemistry and Mechanochemistry SB RASRussian FederationИнститут ядерной физики им. Г.И. Будкера СО РАНРоссияBudker Institute of Nuclear Physics SB RASRussian FederationИнститут ядерной физики им. Г.И. Будкера СО РАН; Центр коллективного пользования «Сибирский кольцевой источник фотонов» Института катализа им. Г.К. Борескова СО РАНРоссияBudker Institute of Nuclear Physics SB RAS; Synchrotron Radiation Facility – Siberian Circular Photon Source “SKlF”, Boreskov Institute of Catalysis SB RASRussian Federation202326122023134494505Copyright © Амброс Е.В., Крупович Е.С., Колмогоров Ю.П., Трофимова Е.Г., Гусев И.С., Гольденберг Б.Г., 20232023Амброс Е.В., Крупович Е.С., Колмогоров Ю.П., Трофимова Е.Г., Гусев И.С., Гольденберг Б.Г.Ambros E.V., Krupovich E.S., Kolmogorov Y.P., Trofimova E.G., Gusev I.S., Goldenberg B.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1119

Значительный интерес среди биостимуляторов вызывают кремнийсодержащие препараты в связи с защитной ролью кремния в растениях от неблагоприятных факторов среды. В данной работе в качестве источника кремния использован механокомпозит из шелухи риса и зеленого чая, содержащий растворимые хелатные комплексы диоксида кремния. Целью исследования являлось изучение влияния хелатов кремния на ростовые, физиологические показатели и содержание химических элементов в растениях Fragaria × ananassa (сорт Солнечная полянка) в условиях теплицы. Растения поливали водой без механокомпозита (контроль) или водным раствором, содержащим 0,3 г/л механокомпозита дважды за период. Отбор образцов проводили через неделю после последней обработки. Для определения концентрации химических элементов (Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Cu, Zn, Br, Rb, Sr, Mo) в корнях и побегах земляники садовой предложено применение метода рентгенофлуоресцентного анализа на синхротронном излучении. Показано увеличение содержания хлорофилла a, суммы хлорофиллов а и b, каротиноидов, уменьшение содержания пероксида водорода и увеличение активности основных антиоксидантных ферментов (супероксиддисмутазы, каталазы, пероксидазы) под действием механокомпозита. Определено, что кремний накапливается под действием препарата преимущественно в побегах и оказывает влияние на аккумуляцию микро- и макроэлементов в побегах и корнях растений. Полученные результаты обосновывают использование кремнийсодержащей «зеленой химии» в качестве средств управления ростом и развитием растений земляники садовой в условиях in vivo.

Due to the protective role played by silicon in plants against unfavorable environmental conditions, siliconcontaining preparations are of considerable interest as biostimulants. In this work, a mechanical composite of rice husk and green tea containing soluble silica chelate complexes was used as the source of silicon. The study aims to examine the effect of silicon chelates on the growth and physiological parameters and the chemical composition of Fragaria × ananassa plants (Solnechnaya Polyanka variety) under greenhouse conditions. The plants were watered using water without a mechanical composite (control) or an aqueous solution containing 0.3 g/L of mechanical composite twice per period. Sampling was carried out one week after the last treatment. In order to determine the concentration of chemical elements (Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Cu, Zn, Br, Rb, Sr, and Mo) in the roots and shoots of garden strawberry, it was proposed to use synchrotron X-ray fluorescence analysis. The use of the mechanical composite was shown to increase the amount of chlorophyll a, chlorophylls a and b, and carotenoids; decrease the amount of hydrogen peroxide; and increase the activity of the main antioxidant enzymes (superoxide dismutase, catalase, and peroxidase). It was determined that under the effect of the preparation, silicon accumulates primarily in shoots, affecting the accumulation of micro- and macroelements in the shoots and roots of plants. The obtained results substantiate the use of silicon-containing “green chemistry” as a means of controlling the growth and development of garden strawberry plants under in vivo conditions.

Fragaria × ananassaхелаты кремнияростфизиологические показателирентгенофлуоресцентный анализсинхротронное излучениеFragaria × ananassasilicon chelatesgrowthphysiological dataX-ray fluorescence analysissynchrotron radiationДля проведения исследований использованы материалы уникальной научной установки «Коллекции живых растений в открытом и закрытом грунте» Центрального сибирского ботанического сада СО РАН (USU 440534). Измерения элементного состава методом рентгенофлуоресцентного анализа с использованием синхротронного излучения проводились на оборудовании Центра коллективного пользования «Сибирский центр синхротронного и терагерцового излучения» на базе уникальной научной установки «Комплекс ВЭПП-4 – ВЭПП-2000» в Институте ядерной физики им. Г.И. Будкера СО РАН. 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The authors declare that there are no conflicts of interest present.