vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/achb.918IKYKFLvuzbiochemi-1233Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYПроблемы конструирования тканеинженерных структур на основе коллагенов и некоторые подходы к их решению: обзорIssues in the design of tissue-engineered collagen constructs and some approaches to their solution: A reviewhttps://orcid.org/0000-0003-2723-6569ФарионИ. А.FarionI. A.

Иван Александрович Фарион, к. х. н., научный сотрудник

670047; ул. Сахьяновой, 6; Улан-Удэ

Ivan A. Farion, Cand. Sci. (Chemistry), Researcher

670047; 6, Sakhyanovoy St.; Ulan-Ude

fariv@mail.ruhttps://orcid.org/0000-0003-3104-3591БурдуковскийВ. Ф.BurdukovskiiV. F.

Виталий Федорович Бурдуковский, д. х. н., доцент, заместитель директора по научной работе

670047; ул. Сахьяновой, 6; Улан-Удэ

Vitalii F. Burdukovskii, Dr. Sci. (Chemistry), Associate Professor, Deputy Director for Research

670047; 6, Sakhyanovoy St.; Ulan-Ude

burdvit@mail.ruБайкальский институт природопользования СО РАНРоссияBaikal Institute of Nature Management SB RASRussian Federation202407072024142195206Copyright © Фарион И.А., Бурдуковский В.Ф., 20242024Фарион И.А., Бурдуковский В.Ф.Farion I.A., Burdukovskii V.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1233

   Целью проведенной работы являлся анализ современных литературных источников, посвященных исследованию проблем дизайна биочернил и тканеинженерных конструкций на основе растворимых форм коллагена, включая желатин.

   Выбор растворимых форм коллагена в качестве биополимерной основы для биочернил и такого типа конструкций определяется их уникальной биосовместимостью, биорезорбируемостью, наличием адгезионных мест (мотивов) для связывания клеток с последующей их пролиферацией и созреванием органов или тканей. В то же время плохие механические свойства изделий из растворимых коллагенов, быстрая биодеградация, склонность к потере растворимости высоковязких растворов при хранении или увеличении pH ограничивают их применение в тканевой инженерии. Применение более стабильных низковязких коллагеновых растворов не позволяет создать размерно-стабильные тканеинженерные конструкции. Показано, что включение в состав гидрогелей на основе растворимых коллагенов различных водорастворимых биосовместимых полимерных добавок позволяет решить вышеперечисленные проблемы, а также дает возможность настраивать требуемые характеристики биочернил и тканеинженерных конструкций. Среди добавок, улучшающих их характеристики, следует выделить биополимеры: серицин и фиброин шелка, а также альгинаты и фибриноген, способные образовывать сшивки в присутствии Ca2+. Показано, что посредством такого типа сшивок можно в еще большей степени улучшить эксплуатационные характеристики данных конструкций. Все эти биополимеры доступны как коммерческие продукты. Проведен сравнительный анализ подходов к стабилизации формы, улучшению механических свойств, а также настраиванию времени биорезорбции напечатанных тканеинженерных конструкций при созревании органа или ткани.

   This review article analyzes modern literature sources on the design of bioinks and tissue-engineered constructs on the basis of soluble forms of collagen, including gelatin.

   The choice of soluble forms of collagen as a biopolymer basis for bioinks and this type of constructs is determined by their unique biocompatibility, bioresorbability, as well as the presence of adhesive sites (motifs) for binding cells with their subsequent proliferation and organ or tissue maturation. However, the poor mechanical properties of products derived from soluble collagens, rapid biodegradation, tendency to lose the solubility of highly viscous solutions when stored or with pH increase limit their application in tissue engineering. The use of more stable low-viscosity collagen solutions does not enable the creation of dimensionally stable tissue-engineered constructs. It is shown that the introduction of various water-soluble biocompatible polymeric additives into hydrogels on the basis of soluble collagens allows the above-mentioned problems to be solved, as well as providing a means to customize the required characteristics of bioinks and tissue-engineered constructs. The additives that improve their characteristics include biopolymers: silk sericin and fibroin, as well as alginates and fibrinogen, which can form cross-links in the presence of Ca2+. This type of crosslinking is shown to further improve the performance of these constructs. All of these biopolymers are commercially available. The article comparatively analyzes approaches to stabilizing the shape, improving the mechanical properties, and adjusting the bioresorption time of 3D printed tissue-engineered constructs during organ or tissue maturation.

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The authors declare that there are no conflicts of interest present.