vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/achb.922PXLPLWvuzbiochemi-1239Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYАнтиоксидантные свойства сверхкритических экстрактов бурых водорослейAntioxidant properties of supercritical extracts of brown algaehttps://orcid.org/0000-0002-7068-911XТабакаеваО. В.TabakaevaO. V.

Оксана Вацлавовна Табакаева, д. т. н., доцент, профессор, заведующий лабораторией

Институт биотехнологий, биоинженерии и пищевых систем

690922; о. Русский, п. Аякс, 10; Владивосток

Oksana V. Tabakaeva, Dr. Sci. (Engineering), Professor, Head of the Laboratory

Institute of Biotechnology, Bioengineering and Food Systems

690922; 10, Ajax Village, Russkii Isl.; Vladivostok

yankovskaya68@mail.ruhttps://orcid.org/0000-0001-5658-5069ТабакаевА. В.TabakaevA. V.

Антон Вадимович Табакаев, к. т. н., доцент, научный сотрудник

Институт биотехнологий, биоинженерии и пищевых систем

690922;  о. Русский, п. Аякс, 10; ул. Сельская, 10; Владивосток

Anton V. Tabakaev, Cand. Sci. (Engineering), Associate Professor, Researcher

Institute of Biotechnology, Bioengineering and Food Systems

690922; 10, Ajax Village, Russkii Isl.; 10, Selskaya St.; Vladivostok

tabakaev92@mail.ruhttps://orcid.org/0000-0002-0365-6342СилантьевВ. Е.Silant’evV. E.

Владимир Евгеньевич Силантьев, к. х. н., директор Департамента

Школа медицины и наук о жизни; Департамент биомедицинской химии

690922; о. Русский, п. Аякс, 10; Владивосток

Vladimir E. Silant’ev, Cand. Sci. (Chemistry), Director of the Department

School of Medicine and Life Sciences

690922; 10, Ajax Village, Russkii Isl.; Vladivostok

silantev.ve@dvfu.ruhttps://orcid.org/0009-0005-8740-1249КапустаС. В.KapustaS. V.

Светлана Владимировна Капуста, аспирант

690922; о. Русский, п. Аякс, 10; Владивосток

Svetlana V. Kapusta, Postgraduate Student

690922; 10, Ajax, Russky Island; Vladivostok

kapusta.sv@dvfu.ruДальневосточный федеральный университетРоссияFar Eastern Federal UniversityRussian FederationДальневосточный федеральный университет; Научно-исследовательский институт эпидемиологии и микробиологии им. Г.П. Сомова Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человекаРоссияFar Eastern Federal University; Research Institute of Epidemiology and Microbiology named after G.P. Somov Federal Service for Supervision in the Field of Protection of Consumer Rights and Human Well-BeingRussian Federation202407072024142253264Copyright © Табакаева О.В., Табакаев А.В., Силантьев В.Е., Капуста С.В., 20242024Табакаева О.В., Табакаев А.В., Силантьев В.Е., Капуста С.В.Tabakaeva O.V., Tabakaev A.V., Silant’ev V.E., Kapusta S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1239

   Морские водоросли являются уникальным сырьем, которое способно в достаточно короткие сроки формировать значительную биомассу, осуществлять синтез разнообразных химических соединений, в том числе и специфических биологически активных веществ, проявляющих различную биологическую активность. Изучение антиоксидантных, антибактериальных, антивирусных, противовоспалительных и других свойств биологически активных веществ бурых водорослей является актуальной задачей.

   Целью представленного исследования являлась оценка антиоксидантных свойств сверхкритических экстрактов бурых водорослей Saccharina japonica и Ascophyllum nodosum.

   Содержание каротиноидов, фенольных соединений, маннита определяли спектрофотометрическим методом. Исследование содержания металлов осуществляли с применением метода атомно-абсорбционной спектрометрии. Антиоксидантную активность оценивали по антирадикальной активности с использованием радикала 2,2-дифенил-1-пикрилгидразила, гидроксил-ион связывающей и Fe+2 хелатирующей активностям, а также по активности поглощения супероксидных радикалов. В исследованных сверхкритических экстрактах бурых водорослей определено достаточно высокое содержание жирных кислот и фенольных соединений, причем жирные кислоты максимально представлены в сверхкритическом экстракте Ascophyllum nodosum, а фенолы, маннит и пигменты – в экстракте Saccharina japonica. Преобладающим макроэлементом в сверхкритических экстрактах бурых водорослей является калий с максимумом содержания в экстракте Saccharina japonica. Сверхкритические экстракты бурых водорослей являются безопасными по содержанию нитрозаминов, полихлорированных бифенилов, токсичных элементов и радионуклидов. Наиболее выраженные антирадикальные свойства продемонстрировал сверхкритический экстракт Saccharina japonica. Самая высокая гидроксил-ион связывающая активность отмечена для сверхкритического экстракта Saccharina japonica. В отношении активности поглощения супероксидных радикалов закономерности были иными: максимальную активность продемонстрировал экстракт Ascophyllum nodosum. Fe+2 хелатирующая активность для сверхкритических экстрактов бурых водорослей была примерно одинаковой, максимальная (38,7 %) зафиксирована для экстракта Ascophyllum nodosum.

   Macroalgae is a unique feedstock capable of producing significant biomass in a fairly short time and synthesizing various chemical compounds, including specific biologically active substances that exhibit different biological activity. The study of antioxidant, antibacterial, antiviral, anti-inflammatory, and other properties of the biologically active substances of brown algae constitutes a relevant problem.

   The present study aims to evaluate the antioxidant properties of supercritical extracts of the brown algae Saccharina japonica and Ascophyllum nodosum.

   The content of carotenoids, phenolic compounds, and mannitol was determined using a spectrophotometric method. The metal content was analyzed using atomic absorption spectrometry. The antioxidant activity was evaluated according to antiradical activity using a 2,2-diphenyl-1-picrylhydrazyl radical, hydroxyl ion binding activity, and Fe+2 chelating activity, as well as the superoxide radical scavenging activity. The studies on the supercritical extracts of brown algae revealed a rather high content of fatty acids and phenolic compounds, with fatty acids most abundantly present in the supercritical extract of Ascophyllum nodosum; phenols, mannitol, and pigments, in the extract of Saccharina japonica. The predominant macroelement in the supercritical extracts of brown algae is potassium, with the maximum content found in the extract of Saccharina japonica. The supercritical extracts of brown algae are safe in terms of the contents of nitrosamines, polychlorinated biphenyls, toxic elements, and radionuclides. The most pronounced antiradical properties were shown by the supercritical extract of Saccharina japonica. The highest hydroxyl ion binding activity was noted in the supercritical extract of Saccharina japonica. In terms of superoxide radical scavenging activity, the maximum activity was exhibited by the extract of Ascophyllum nodosum. The Fe+2 chelating activity was about the same for the supercritical extracts of brown algae, with the maximum activity (38.7 %) observed for the extract of Ascophyllum nodosum.

сверхкритические экстрактыбурые водорослиантиоксидантные свойстваантирадикальная активностьsupercritical extractsbrown algaeantioxidant propertiesantiradical activityИсследования объекта Ascophyllum nodosum выполнены при поддержке Российского научного фонда (проект № 23-26-00197). Исследования объекта Saccharina japonica выполнены при поддержке проекта «Науки о жизни: Биомедицина» программы стратегического академического лидерства «Приоритет-2030», инициативная тематика № 23-01-3.04.-0010 «Биофармацевтические технологии для персонализированной медицины и здоровьесбережения»Studies of the Ascophyllum nodosum object were carried out with the support of the RGNF (project no. 23-26- 00197). 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The authors declare that there are no conflicts of interest present.