References

vuzbiochemi

Известия вузов. Прикладная химия и биотехнология

Proceedings of Universities. Applied Chemistry and Biotechnology

2227-29252500-1558

ИРНИТУ

.org/10.21285/2227-2925-2018-8-3-33-40

vuzbiochemi-128

Research Article

ФИЗИКО-ХИМИЧЕСКАЯ И ОБЩАЯ БИОЛОГИЯ

PHYSICOCHEMICAL AND GENERAL BIOLOGY

ВЛИЯНИЕ УСЛОВИЙ КУЛЬТИВИРОВАНИЯ НА БИОСИНТЕЗ БАКТЕРИАЛЬНОЙ НАНОЦЕЛЛЮЛОЗЫ

EFFECTS OF CULTIVATION CONDITIONS ON THE BIOSYNTHESIS OF BACTERIAL NANOCELLULOSE

Гладышева

Е. К.

Gladysheva

Е. К.

evg-gladysheva@yandex.ru

Институт проблем химико-энергетических технологий СО РАНРоссияInstitute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS)Russian Federation

2018

23092019

833340

2019

Гладышева Е.К.

Gladysheva Е.К.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vuzbiochemi.elpub.ru/jour/article/view/128

Представлены основные условия культивирования, оказывающие влияние на выход бактериальной наноцеллюлозы при использовании разных продуцентов. В связи с высокой востребованностью в бактериальной наноцеллюлозе в различных отраслях промышленности исследования параметров, обеспечивающих высокий выход, являются актуальными. К основным параметрам, влияющим на рост целлюлозосинтезирующих бактерий и биосинтез бактериальной наноцеллюлозы, можно отнести: концентрацию источника углерода в питательной среде; аэрацию; температуру культивирования; уровень активной кислотности. Концентрация редуцирующих веществ в питательной среде может находится в диапазоне от 6 до 100 г/л. Концентрацию растворенного кислорода в питательной среде можно считать лимитирующим фактором для всех целлюлозосинтезирующих микроорганизмов. Температурный диапазон для биосинтеза бактериальной наноцеллюлозы у разных продуцентов может варьировать от 25 до 33 °С. Диапазон pH, обеспечивающий наибольший выход бактериальной наноцеллюлозы, для разных продуцентов изменяется от 4 до 6,5. Обзор литературы показал необходимость подбора условий культивирования, обеспечивающих максимальный выход бактериальной наноцеллюлозы для каждого продуцента отдельно.

This paper describes conditions for the cultivation of bacterial nanocellulose using various primary producers. Research into parameters determining a high yield in the production of bacterial nanocellulose is highly relevant due to an increased demand in this product in various industries. Key parameters that affect the growth of cellulose-synthesizing bacteria and the biosynthesis of bacterial nanocellulose include the following: the concentration of the carbon source in the nutrient solution; aeration; cultivation temperature; active acidity level. The concentration of reducing substances in the nutrient solution can range from 6 to 100 g/l. The concentration of dissolved oxygen in the nutrient solution can be considered as a limiting factor for all cellulose-synthesizing microorganisms. It is shown that the temperature range for the bacterial nanocellulose biosynthesis can vary from 25 to 33°C for various primary producers. PH values that provide a maximal yield of bacterial nanocellulose are determined to range from 4 to 6.5 for various primary producers. The literature review has proven the importance of a case-by-case approach when selecting cultivation conditions for every primary producer so as to maximize the bacterial nanocellulose yield.

бактериальная наноцеллюлозапродуцентвыход продуктааэрациятемпературауровень активной кислотности

bacterial nanocellulosecellulose-producing bacteriumproduct yieldaerationtemperaturepH level

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The authors declare that there are no conflicts of interest present.