vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/achb.930ONJTWUvuzbiochemi-1285Research ArticleКРАТКИЕ СООБЩЕНИЯBRIEF COMMUNICATIONФизико-химическая характеристика меланинов культуральной среды Inocutis dryophila (Berk) Flasson & NiemelaPhysico-chemical characteristics of melanins culture medium Inocutis dryophila (Berk) Flasson & Niemelahttps://orcid.org/0000-0002-1120-2148ГорностайТ. Г.GornostaiT. G.

Горностай Татьяна Геннадьевна - к.фарм.н., научный сотрудник.

664033, Иркутск, ул. Лермонтова, 132

Tatyana G. Gornostai - Cand. Sci. (Pharmaceutics), Researcher.

132, Lermontov St., Irkutsk, 664033

t.g.gornostay@yandex.ruСибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and Biochemistry SB RASRussian Federation202430092024143416420Copyright © Горностай Т.Г., 20242024Горностай Т.Г.Gornostai T.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1285

Целью настоящего исследования явилось изучение влияния световой обработки на синтез эндомеланинов мицелия Inocutis dryophila в жидкую культуральную среду. Для выявления действия света на синтез меланинов проводили культивирование мицелия Inocutis dryophila на жидкой среде в темноте и с применением обработки синими светодиодами. В результате были получены два вида меланинов IDM-1 и IDM-2. Установлено, что световой фактор оказывает влияние на количественный выход меланинов из мицелия Inocutis dryophila в культуральную жидкость. Применение синего света приводило к меньшему содержанию меланина в культуральной жидкости по сравнению с количеством меланина, полученного в темноте. Методом ультрафиолетовой и инфракрасной спектрометрии выявлено, что оба вида меланинов имеют типичные спектры и графики для грибных меланинов. В ходе инфракрасной спектрометрии было обнаружено, что обработка мицелия синим светом приводила к деформации молекул меланинов IDM-1. Установлено, что меланины IDM-2 проявляли большую антирадикальную активность, чем меланины IDM-1. Таким образом, при глубинном культивировании световая обработка мицелия Inocutis dryophila оказывает влияние на количественный выход меланинов в культуральную жидкость, вносит вклад в изменение структуры, а также проявление биологического эффекта.

The purpose of the study was to investigate the effect of light treatment on the synthesis of endomelanins from the mycelium of Inocutis dryophila in a liquid culture medium. To identify the effect of light on the synthesis of melanins, the mycelium of Inocutis dryophila was cultivated in a liquid medium in the dark and using blue light treatment. As a result, two types of melanins IDM-1 and IDM-2 were obtained. It has been established that the light factor influences the quantitative release of melanins from the mycelium of Inocutis dryophila into the culture liquid. The use of blue light resulted in lower melanin content in the culture fluid than in the dark. Ultraviolet and infrared spectrometry showed that both types of melanins have typical spectra and graphs for fungal melanins. Infrared spectrometry showed that treatment of mycelium with blue light led to deformation of IDM-1. It was revealed that IDM-2 melanins exhibit greater antiradical activity than IDM-1 melanins. Thus, light treatment of Inocutis dryophila mycelium in a liquid medium affects the quantitative release of melanins into the culture liquid, promotes a change in the structure, as well as manifestation of a biological effect.

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The authors declare that there are no conflicts of interest present.