vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/achb.943XNVAAGvuzbiochemi-1339Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВлияние повышенной экспрессии гена митохондриальной альтернативной внешней NADH-дегидрогеназы Arabidopsis thaliana на уровень генерации активных форм кислорода в листьях табака Nicotiana tabacum при низкой температуреEffect of increased gene expression of alternative external NADH dehydrogenase of mitochondria of Arabidopsis thaliana on the generation of reactive oxygen in Nicotiana tabacum tobacco leaves at low temperatureshttps://orcid.org/0000-0002-5089-5311БоровскийГ. Б.BorovskiiG. B.

Боровский Геннадий Борисович, д.б.н., профессор, заместитель директора по научной работе

664033, г. Иркутск, ул. Лермонтова, 132

Gennadii B. Borovskii, Dr. Sci. (Biology), Professor, Vice-director

132, Lermontov St., Irkutsk, 664033

borovskii@sifibr.irk.ruhttps://orcid.org/0000-0002-6858-380XГорбылеваЕ. Л.GorbylevaE. L.

Горбылева Елена Леонидовна, к.б.н., младший научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Elena L. Gorbyleva, Cand. Sci. (Biology), Researcher

132, Lermontov St., Irkutsk, 664033

dzubina@sifibr.irk.ruhttps://orcid.org/0000-0001-7856-0460КатышевА. И.KatyshevA. I.

Катышев Александр Игоревич, к.б.н., старший научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Alexander I. Katyshev, Cand. Sci. (Biology), Senior Researcher

132, Lermontov St., Irkutsk, 664033

byacky78@mail.ruhttps://orcid.org/0000-0003-4236-389XКоротаеваН. Е.KorotaevaN. E.

Коротаева Наталья Евгеньевна, к.б.н., старший научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Natalia E. Korotaeva, Cand. Sci. (Biology), Senior Researcher

132, Lermontov St., Irkutsk, 664033

korotaeva73@sifibr.irk.ruhttps://orcid.org/0000-0002-4830-5888ПоляковаЕ. А.PolyakovaE. A.

Полякова Елизавета Алексеевна, аспирант, ведущий инженер

664033, г. Иркутск, ул. Лермонтова, 132

Elizaveta A. Polyakova, Postgraduate Student, Leading Engineer

132, Lermontov St., Irkutsk, 664033

polyackova.elizaveta727@yandex.ruhttps://orcid.org/0009-0001-0444-0447ПятрикасД. В.PyatrikasD. V.

Пятрикас Дарья Валерьевна, к.б.н., научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Darya V. Pyatrikas, Cand. Sci. (Biology), Researcher

132, Lermontov St., Irkutsk, 664033

galdasova@sifibr.irk.ruhttps://orcid.org/0000-0002-0456-3690СтепановА. В.StepanovA. V.

Степанов Алексей Владимирович, к.б.н., старший научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Alexey V. Stepanov, Cand. Sci. (Biology), Senior Researcher

132, Lermontov St., Irkutsk, 664033

stepanov@sifibr.irk.ruhttps://orcid.org/0000-0001-6529-9304ФедосееваИ. В.FedoseevaI. V.

Федосеева Ирина Владимировна, к.б.н., старший научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Irina V. Fedoseeva, Cand. Sci. (Biology), Senior Researcher

132, Lermontov St., Irkutsk, 664033

fedoseeva.irina2009@yandex.ruhttps://orcid.org/0009-0000-8502-4826ШигароваА. М.ShigarovaA. M.

Шигарова Анастасия Михайловна, к.б.н., научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Anastasiya M. Shigarova, Cand. Sci. (Biology), Researcher

132, Lermontov St., Irkutsk, 664033

anas_shig@mail.ruСибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and Biochemistry SB RASRussian Federation202413012025144514524Copyright © Боровский Г.Б., Горбылева Е.Л., Катышев А.И., Коротаева Н.Е., Полякова Е.А., Пятрикас Д.В., Степанов А.В., Федосеева И.В., Шигарова А.М., 20242024Боровский Г.Б., Горбылева Е.Л., Катышев А.И., Коротаева Н.Е., Полякова Е.А., Пятрикас Д.В., Степанов А.В., Федосеева И.В., Шигарова А.М.Borovskii G.B., Gorbyleva E.L., Katyshev A.I., Korotaeva N.E., Polyakova E.A., Pyatrikas D.V., Stepanov A.V., Fedoseeva I.V., Shigarova A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1339

Низкая температура является одним из важных факторов, лимитирующих жизнеспособность и продуктивность растений. При многих стрессах, в том числе и при низкой температуре, в клетке происходит увеличение генерации активных форм кислорода, которые являются сигнальными молекулами и в то же время вредят клетке, повреждая ее компоненты. В свою очередь, митохондрии представляют собой одну из основных мишеней окислительного повреждения при стрессе, а кроме того, значительный источник активных форм кислорода. Митохондрии растений имеют большое количество ферментов альтернативных путей транспорта электронов, многие из которых активны при стрессе. Цель проведенного исследования заключалась в оценке влияния низкой положительной температуры и повышенной экспрессии гетерологичного гена NDB2 (альтернативная внешняя NADH-дегидрогеназа митохондрий) на генерацию активных форм кислорода, работу альтернативной дыхательной цепи в митохондриях и экспрессию стрессовых белков в условиях освещения в листьях табака Nicotiana tabacum. Установлено, что в листьях растений табака с повышенной экспрессией гена NDB2 Arabidopsis thaliana происходило снижение продукции активных форм кислорода в контрольных условиях и в условиях пониженной температуры в сравнении с растениями дикого типа. Полученные результаты свидетельствуют, что гетерологичный ген NDB2 Arabidopsis thaliana участвует в увеличении активности альтернативной электрон-транспортной цепи в митохондриях растений табака, снижает уровень генерации активных форм кислорода и влияет на содержание стрессовых белков как в контрольных условиях, так и в условиях низкотемпературного воздействия.

Low temperature is an important factor limiting plant viability and productivity. Along with other stresses, low temperatures increase the generation of reactive oxygen species, which are signaling molecules that can damage cell components. As well as representing one of the main targets of oxidative damage during stress, mitochondria represent a significant source of reactive oxygen species. Plant mitochondria have a large number of enzymes providing alternative electron transport pathways, many of which are activated under stress. Our aim was to assess the effect of low positive temperatures and increased expression of the heterologous gene NDB2 (alternative external NADH dehydrogenase of mitochondria) on the generation of reactive oxygen species, which involve an alternative respiratory chain in mitochondria and the expression of stress proteins under lighting conditions in Nicotiana tabacum tobacco leaves. In the leaves of tobacco plants with increased expression of the Arabidopsis thaliana NDB2 (AtNDB2) gene, a decrease in reactive oxygen species production was observed under normal and low temperature conditions. The results indicate that the heterologous Arabidopsis thaliana NDB2 gene is involved in increasing the activity of the alternative electron transport chain in mitochondria, which reduces the level of reactive oxygen species generation and affects the content of stress proteins under normal and low-temperature exposure.

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The authors declare that there are no conflicts of interest present.