vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/achb.942TIVSPZvuzbiochemi-1345Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВлияние биостимулятора Rhodococcus qingshengii VKM Ac-2784D на бактерии, выделенные из эндо- и ризосферы растений картофеляInfluence of Rhodococcus qingshengii VKM Ac-2784D biostimulator on bacterial isolates from potato endo- and rhizospherehttps://orcid.org/0000-0003-2266-3764МорицА. С.MoritsA. S.

Мориц Анна Сергеевна, ведущий инженер

664033, г. Иркутск, ул. Лермонтова, 132

Anna S. Morits, Leading Engineer

132, Lermontov St., Irkutsk, 664033

aconitkaaco@gmail.comhttps://orcid.org/0000-0001-7767-4204МарковаЮ. А.MarkovaYu. A.

Маркова Юлия Александровна, д.б.н., главный научный сотрудник, заведующий лабораторией

664033, г. Иркутск, ул. Лермонтова, 132

Yuliya A. Markova, Dr. Sci. (Biology), Chief Researcher, Head of the Laboratory

132, Lermontov St., Irkutsk, 664033

juliam06@mail.ruhttps://orcid.org/0000-0002-0328-6716ФилиноваН. В.FilinovaN. V.

Филинова Надежда Владимировна, к.б.н., ведущий технолог

664033, г. Иркутск, ул. Лермонтова, 132

Nadezhda V. Filinova, Cand. Sci. (Biology), Leading Technologist

132, Lermontov St., Irkutsk, 664033

filinova_nv@mail.ruhttps://orcid.org/0000-0002-8788-5352ПетрушинИ. С.PetrushinI. S.

Петрушин Иван Сергеевич, к.т.н., научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Ivan S. Petrushin, Cand. Sci. (Engineering), Researcher

132, Lermontov St., Irkutsk, 664033

ivan.kiel@gmail.comСибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and Biochemistry SB RASRussian Federation202413012025144578585Copyright © Мориц А.С., Маркова Ю.А., Филинова Н.В., Петрушин И.С., 20242024Мориц А.С., Маркова Ю.А., Филинова Н.В., Петрушин И.С.Morits A.S., Markova Y.A., Filinova N.V., Petrushin I.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1345

Микробиом картофеля оказывает существенное влияние на рост и развитие растений. Для воздействия на микробиом в сельском хозяйстве перспективно использовать различные биопрепараты на основе почвенных микроорганизмов. Растения картофеля, будучи уязвимыми к патогенам и засухе, наиболее интересны в качестве объекта для разработки биопрепаратов. Особое внимание уделяется бактериям рода Rhodococcus благодаря их способности очищать почву от загрязнений и стимулировать рост растений. Целью проведенного исследования являлось изучение влияния штамма Rhodococcus qingshengii VKM Ac-2784D на бактерии, выделенные из эндо- и ризосферы растений картофеля. Известно, что лишь малая часть микроорганизмов в составе растительного микробиома может быть получена в чистой культуре. С учетом этих ограничений без использования селективных сред удалось выделить более 70 эндофитных штаммов и показать, что многие из них чувствительны к присутствию компонента биопрепарата из Rhodococcus qingshengii VKM Ac-2784D. Данные метагеномного исследования свидетельствуют об изменении состава микробного сообщества после обработки биопрепаратом. Эксперименты также показывают, что бактерии сохраняют чувствительность к родококку даже в присутствии других конкурирующих штаммов. В целом результаты проведенного исследования указывают на модулирующее воздействие биопрепарата на микробиом картофеля без фитотоксичности. Полученные сведения важны для понимания влияния биопрепаратов на микробный состав почвы и растений картофеля, а также для разработки эффективных стратегий использования микроорганизмов в сельском хозяйстве.

The potato microbiome has a significant impact on plant growth and development. In order to affect this microbiome, agriculture can use various biopreparations on the basis of soil microorganisms. Being vulnerable to pathogens and drought, potato plants are particularly useful in the development of biopreparations. Special attention is given to Rhodococcus bacteria due to their ability to clean contaminated soil and stimulate plant growth. The present study was aimed at examining the effect of Rhodococcus qingshengii VKM Ac-2784D on bacteria isolated from potato endo- and rhizosphere. It is known that only a small fraction of microorganisms within the plant microbiome can be obtained in pure culture. Given these limitations, it was possible to isolate over 70 endophytic strains without the use of selective media and show that many of them are sensitive to the presence of a biopreparation component on the basis of Rhodococcus qingshengii VKM Ac-2784D. The metagenomic study indicates a change in the composition of the microbial community following treatment with the biopreparation. The experiments also show that the bacteria remain sensitive to Rhodococcus even in the presence of other competing strains. In general, the study results indicate a modulating effect of the biopreparation on the potato microbiome without phytotoxicity. The findings are important for understanding the effect of the biopreparation on the microbial composition of soil and potato plants, as well as for developing effective strategies for the use of microorganisms in agriculture.

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The authors declare that there are no conflicts of interest present.