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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vuzbiochemi</journal-id><journal-title-group><journal-title xml:lang="ru">Известия вузов. Прикладная химия и биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of Universities. Applied Chemistry and Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-2925</issn><issn pub-type="epub">2500-1558</issn><publisher><publisher-name>ИРНИТУ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21285/achb.968</article-id><article-id custom-type="edn" pub-id-type="custom">XDMZZP</article-id><article-id custom-type="elpub" pub-id-type="custom">vuzbiochemi-1414</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PHYSICOCHEMICAL BIOLOGY</subject></subj-group></article-categories><title-group><article-title>Изменение некоторых агрофизических характеристик нефтезагрязненных почв после их обработки растворами полисорбата-80</article-title><trans-title-group xml:lang="en"><trans-title>Changes in some agrophysical characteristics of oil-contaminated soils following their washing with polysorbate 80 solutions</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0006-4762-4613</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рюмин</surname><given-names>М. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Ryumin</surname><given-names>M. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Рюмин Максим Борисович, аспирант</p><p>664003, г. Иркутск, ул. Карла Маркса, 1</p></bio><bio xml:lang="en"><p>Maxim B. Ryumin, Postgraduate Student</p><p>1, Karl Marx St., Irkutsk, 664003</p></bio><email xlink:type="simple">maksim.ryumin@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5570-545X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лопатовская</surname><given-names>О. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Lopatovskaya</surname><given-names>O. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лопатовская Ольга Геннадьевна, д.б.н., профессор, заведующий кафедрой</p><p>664003, г. Иркутск, ул. Карла Маркса, 1</p></bio><bio xml:lang="en"><p>Olga G. Lopatovskaya, Dr. Sci. (Biology), Professor, Head of the Department</p><p>1, Karl Marx St., Irkutsk, 664003</p></bio><email xlink:type="simple">lopatovs@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9496-2961</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Стом</surname><given-names>Д. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Stom</surname><given-names>D. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Стом Дэвард Иосифович, д.б.н., профессор, главный научный сотрудник</p><p>664520, п. Листвянка, ул. Академическая, 1;</p><p>Профессор</p><p>664003, г. Иркутск, ул. Карла Маркса, 1;</p><p>Профессор</p><p>664074, г. Иркутск, ул. Лермонтова, 83;</p><p>ведущий научный сотрудник</p><p>628412, г. Сургут, пр. Ленина, 1</p></bio><bio xml:lang="en"><p>Devard I. Stom, Dr. Sci. (Biology), Professor, Chief Researcher</p><p>1, Academicheskaya St., Listvyanka, 664520;</p><p>Professor</p><p>1, Karl Marx St., Irkutsk, 664003;</p><p>Professor</p><p>83, Lermontov St., Irkutsk, 664074;</p><p>Leading Researcher</p><p>1, Lenin Ave., Surgut, 628412</p></bio><email xlink:type="simple">stomd@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0326-7224</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чеснокова</surname><given-names>А. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Chesnokova</surname><given-names>A. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Чеснокова Александра Николаевна, к.х.н., доцент, доцент, заведующий лабораториями</p><p>664074, г. Иркутск, ул. Лермонтова, 83</p></bio><bio xml:lang="en"><p>Alexandra N. Chesnokova, Cand. Sci. (Chemistry), Associate Professor, Associate Professor, Head of Laboratories</p><p>83, Lermontov St., Irkutsk, 664074</p></bio><email xlink:type="simple">chesnokova@istu.edu</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9474-0568</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сутормин</surname><given-names>О. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Sutormin</surname><given-names>O. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сутормин Олег Сергеевич, к.б.н., заведующий кафедрой</p><p>628412, г. Сургут, пр. Ленина, 1</p></bio><bio xml:lang="en"><p>Oleg S. Sutormin, Cand. Sci. (Biology), Head of the Department</p><p>1, Lenin Ave., Surgut, 628412</p></bio><email xlink:type="simple">sutormin_os@surgu.ru</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8884-8636</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Купчинский</surname><given-names>А. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Kupchinsky</surname><given-names>A. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Купчинский Александр Борисович, к.б.н., директор</p><p>664520, п. Листвянка, ул. Академическая, 1</p></bio><bio xml:lang="en"><p>Alexander B. Kupchinsky, Cand. Sci. (Biology), Director</p><p>1, Academicheskaya St., Listvyanka, 664520</p></bio><email xlink:type="simple">albor67@mail.ru</email><xref ref-type="aff" rid="aff-5"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5217-7815</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Алферов</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Alferov</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Алферов Сергей Валерьевич, к.х.н., доцент, заведующий лабораторией</p><p>300012, г. Тула, ул. Фридриха Энгельса, 157</p></bio><bio xml:lang="en"><p>Sergey V. Alferov, Cand. Sci. (Chemistry), Associate Professor, Head of the Laboratory</p><p>157, Friedrich Engels St., Tula, 300012</p></bio><email xlink:type="simple">s.v.alferov@gmail.com</email><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3702-2249</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Петрова</surname><given-names>Ю. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Petrova</surname><given-names>Yu. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Петрова Юлия Юрьевна, к.х.н., доцент, директор Института естественных и технических наук</p><p>628412, г. Сургут, пр. Ленина, 1</p></bio><bio xml:lang="en"><p>Yulia Yu. Petrova, Cand. Sci. (Chemistry), Associate Professor, Director of the Institute of Natural and Technical Sciences</p><p>1, Lenin Ave., Surgut, 628412</p></bio><email xlink:type="simple">petrova_juju@surgu.ru</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4735-2033</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Есимсиитова</surname><given-names>З. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Yessimsiitova</surname><given-names>Z. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Есимсиитова Зура Беркутовна, к.б.н., доцент</p><p>050040, г. Алматы, пр. аль-Фараби, 71;</p><p>главный научный сотрудник</p><p>050014, г. Алматы, ул. Павлодарская, 11</p></bio><bio xml:lang="en"><p>Zura B. Yessimsiitova, Cand. Sci. (Biology), Associate Professor</p><p>71, al-Farabi Ave., Almaty, 050040;</p><p>Chief Researcher</p><p>11, Pavlodarskaya St., Almaty, 050014</p></bio><email xlink:type="simple">zura1958@bk.ru</email><xref ref-type="aff" rid="aff-7"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5831-123X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Федина</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Fedina</surname><given-names>V. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Федина Вероника Вячеславовна, ассистент, младший научный сотрудник</p><p>300012, г. Тула, ул. Фридриха Энгельса, 157</p></bio><bio xml:lang="en"><p>Veronika V. Fedina, Assistant, Junior Researcher</p><p>157, Friedrich Engels St., Tula, 300012</p></bio><email xlink:type="simple">agapovaweronica@yandex.ru</email><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0008-4228-778X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Артеменко</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Artemenko</surname><given-names>Yu. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Артеменко Юлия Владимировна, аспирант</p><p>664003, г. Иркутск, ул. Карла Маркса, 1</p></bio><bio xml:lang="en"><p>Yulia V. Artemenko, Postgraduate Student</p><p>1, Karl Marx St., Irkutsk, 664003</p></bio><email xlink:type="simple">juliapixell@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Иркутский государственный университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Irkutsk State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Байкальский музей СО РАН;&#13;
Иркутский государственный университет;&#13;
Иркутский национальный исследовательский технический университет;&#13;
Сургутский государственный университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Baikal Museum SB RAS;&#13;
Irkutsk State University;&#13;
Irkutsk National Research Technical University;&#13;
Surgut State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Иркутский национальный исследовательский технический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Irkutsk National Research Technical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Сургутский государственный университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Surgut State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru"><institution>Байкальский музей СО РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Baikal Museum SB RAS</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-6"><aff xml:lang="ru"><institution>Тульский государственный университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Tula State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-7"><aff xml:lang="ru"><institution>Казахский национальный университет им. аль-Фараби;&#13;
Научный производственно-технический центр «Жалын»</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>Al-Farabi Kazakh National University;&#13;
Scientific Production-Technical Center “Jalyn”</institution><country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>07</day><month>04</month><year>2025</year></pub-date><volume>15</volume><issue>1</issue><fpage>67</fpage><lpage>81</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Рюмин М.Б., Лопатовская О.Г., Стом Д.И., Чеснокова А.Н., Сутормин О.С., Купчинский А.Б., Алферов С.В., Петрова Ю.Ю., Есимсиитова З.Ю., Федина В.В., Артеменко Ю.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Рюмин М.Б., Лопатовская О.Г., Стом Д.И., Чеснокова А.Н., Сутормин О.С., Купчинский А.Б., Алферов С.В., Петрова Ю.Ю., Есимсиитова З.Ю., Федина В.В., Артеменко Ю.В.</copyright-holder><copyright-holder xml:lang="en">Ryumin M.B., Lopatovskaya O.G., Stom D.I., Chesnokova A.N., Sutormin O.S., Kupchinsky A.B., Alferov S.V., Petrova Y.Y., Yessimsiitova Z.B., Fedina V.V., Artemenko Y.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vuzbiochemi.elpub.ru/jour/article/view/1414">https://vuzbiochemi.elpub.ru/jour/article/view/1414</self-uri><abstract><p>Целью проведенной работы являлось сопоставление влияния нефти, дизельного топлива и моторного масла на капиллярную влагоемкость и гигроскопическую влажность серой лесной почвы, а также оценка изменения этих параметров нефтезагрязненных почв после обработки полисорбатом-80. В почву вносили воду, промывной раствор (с концентрацией детергента 1, 5 и 10 г/л) и непрерывно перемешивали на шейкере (60 об/мин) в течение 1 ч при 20 °С. Показано снижение исследуемых агрофизических параметров почв при их загрязнении нефтью, дизельным топливом и моторным маслом в концентрациях 50, 150 и 300 мл/кг. При этом действие моторного масла на капиллярную влагоемкость было более выраженным, чем влияние нефти и дизельного топлива в аналогичных концентрациях, а на гигроскопическую влажность – наоборот, менее выраженным. Промывка загрязненных почв полисорбатом-80 частично восстанавливала эти агрофизические параметры, однако они оставались ниже, чем у исходных образцов. Эффективность полисорбата-80 подтверждает и снижение фитотоксичности загрязненных почвенных проб после промывки растворами детергента. При этом при уровне загрязнения почвы нефтепродуктами до 150 мл/кг более эффективными были растворы, содержащие 5 г/л полисорбата-80, а при уровне загрязнения 300 мл/кг рекомендуется повышать концентрацию детергента до 10 г/л. Рекомендуемое для проведения очистки соотношение почвы, промывного раствора и воды составляет 1:1:4 (по массе). Таким образом, продемонстрирована перспективность использования полисорбата-80 для ослабления негативного влияния нефтезагрязнения почвы.</p></abstract><trans-abstract xml:lang="en"><p>The study was aimed at comparing the effect of oil, diesel fuel, and motor oil on the capillary moisture capacity and hygroscopic moisture content of gray forest soil, as well as assessing changes in these characteristics of oil-contaminated soils following their washing with polysorbate 80. Water and a washing solution (with detergent concentrations of 1, 5, and 10 g/L) were added to the soil to be continuously mixed for 1 h at 20 °С using a shaker (60 rpm). The analyzed agrophysical characteristics of soils are shown to decrease when soils are contaminated with oil, diesel fuel, and motor oil at concentrations of 50, 150, and 300 mL/kg. The effect of motor oil on capillary moisture capacity is more pronounced than those of oil and diesel fuel at similar concentrations, while the effect on hygroscopic moisture content is, conversely, less pronounced. The washing of contaminated soils with polysorbate 80 partially restored these agrophysical characteristics; however, they remained lower than those of the original samples. The effectiveness of polysorbate 80 is also confirmed by the reduction in the phytotoxicity of contaminated soil samples following their washing with detergent solutions. At the level of oil contamination of up to 150 mL/kg, solutions containing 5 g/L of polysorbate 80 were found to be more effective, whereas at 300 mL/kg, it is recommended to increase the concentration of detergent up to 10 g/L. The recommended weight ratio of soil, washing solution, and water for soil washing is 1:1:4. Thus, the prospects for using polysorbate 80 to mitigate the negative impact of oil contamination in soil are demonstrated.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>нефтезагрязнение почв</kwd><kwd>агрофизические параметры почв</kwd><kwd>моторное масло</kwd><kwd>нефть</kwd><kwd>дизельное топливо</kwd><kwd>полисорбат-80</kwd></kwd-group><kwd-group xml:lang="en"><kwd>oil contamination in soil</kwd><kwd>agrophysical characteristics of soils</kwd><kwd>motor oil</kwd><kwd>oil</kwd><kwd>diesel fuel</kwd><kwd>polysorbate 80</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена при финансовой поддержке Министерства науки и высшего образования Российской Федерации в рамках государственного задания № FEWG-2024-0003 «Биокаталитические системы на основе клеток микроорганизмов, субклеточных структур и ферментов в сочетании с наноматериалами».</funding-statement><funding-statement xml:lang="en">This research was funded by the Ministry of Science and Higher Education of the Russian Federation as the part of state assignment no. FEWG-2024-0003 “Biocatalytic platforms based on microbial cells, subcellular structures and enzymes in combination with nanomaterials”.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Sakhaei Z., Riazi M. In-situ petroleum hydrocarbons contaminated soils remediation by polymer enhanced surfactant flushing: mechanistic investigation // Process Safety and Environmental Protection. 2022. Vol. 161. Р. 758–770. DOI: 10.1016/j.psep.2022.03.086.</mixed-citation><mixed-citation xml:lang="en">Sakhaei Z., Riazi M. In-situ petroleum hydrocarbons contaminated soils remediation by polymer enhanced surfactant flushing: mechanistic investigation. Process Safety and Environmental Protection. 2022;161:758-770. 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