vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/achb.998SQKPVOvuzbiochemi-1466Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYШтамм Gordonia amicalis G2 – продуцент поверхностно-активных веществ биологического происхожденияGordonia amicalis G2 strain as a producer of biosurfactantshttps://orcid.org/0000-0003-2736-080XНечаеваИ. А.NechaevaI. A.

Нечаева Ирина Александровна, к.б.н., доцент

300012, г. Тула, пр. Ленина, 92, Российская Федерация

Irina A. Nechaeva, Cand. Sci. (Biology), Associate Professor

92, Lenin Ave., Tula, 300012, Russian Federation

nechaeva1902@gmail.comhttps://orcid.org/0009-0009-2661-1145ОсинаА. Н.OsinaA. N.

Осина Анна Николаевна, магистрант

300012, г. Тула, пр. Ленина, 92, Российская Федерация

Anna N. Osina, Master’s Student

92, Lenin Ave., Tula, 300012, Russian Federation

anya.osina2017@yandex.ruhttps://orcid.org/0009-0001-6883-1543ФилипповаА. С.FilippovaA. S.

Филиппова Анастасия Сергеевна, младший научный сотрудник, Научно-исследовательский центр «БиоХимТех»

300012, г. Тула, пр. Ленина, 92, Российская Федерация

Anastasia S. Filippova, Junior Researcher, BioChemTech Centre

92, Lenin Ave., Tula, 300012, Russian Federation

stasya.filippova.01@gmail.comhttps://orcid.org/0000-0003-0091-5369НамН.В.Т.NamN. V.T.

Нам Нгуен Ван Тхань, магистр, научный сотрудник

740300, г. Хо Ши Мин, ул. Третьего Февраля, район 10, Вьетнам

Nguyen V.T. Nam, Master’s Student, Researcher

3, 3 Thang 2 St., Distr. 10, 740300, Ho Chi Minh City, Vietnam

xungcavn@gmail.comhttps://orcid.org/0000-0002-6035-5933МоЛ. Т.MoL. T.

Мо Лыонг Тхи, к.х.н., заведующий лабораторией

740300, г. Хо Ши Мин, ул. Третьего Февраля, район 10, Вьетнам

Luong T. Mo, Cand. Sci. (Chemistry), Head of the Laboratory

3, 3 Thang 2 St., Distr. 10, 740300, Ho Chi Minh City, Vietnam

luongmo@mail.ruТульский государственный университетРоссияTula State UniversityRussian FederationЮжное отделение Совместного Российско-Вьетнамского Тропического научно-исследовательского и технологического центраВьетнамSouthern Branch of the Joint Russia-Vietnam Tropical Research and Technology CenterViet Nam202517062025152213223Copyright © Нечаева И.А., Осина А.Н., Филиппова А.С., Нам Н.V., Мо Л.Т., 20252025Нечаева И.А., Осина А.Н., Филиппова А.С., Нам Н., Мо Л.Т.Nechaeva I.A., Osina A.N., Filippova A.S., Nam N.V., Mo L.T.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/1466

Целью проведенного исследования являлось изучение способности нового штамма-деструктора парафинов Gordonia amicalis G2, выделенного из загрязненного песка морского порта во Вьетнаме, продуцировать биосурфактанты. Мониторинг продукции биосурфактантов штаммом Gordonia amicalis G2 проводили в процессе роста бактерий при периодическом культивировании с использованием гексадекана в качестве ростового субстрата. Эффективность действия продуцируемых биосурфактанов оценивали с помощью поверхностного и межфазного натяжения. Поверхностное и межфазное натяжение растворов биосурфактантов определяли методом отрыва кольца де Нуи с использованием тензиометра Kruss K6. Поверхностное натяжение снизилось до 39 мН/м, межфазное натяжение – до 2 мН/м. Для характеристики химической структуры биосурфактантов использовали тонкослойную хроматографию и инфракрасную спектроскопию с преобразованием Фурье. По результатам качественной оценки выявлена гликолипидная природа вторичных метаболитов. Критическую концентрацию мицеллообразования определяли по точке перегиба на кривых зависимостях поверхностного натяжения от содержания биосурфактанта. Значение критической концентрации мицеллообразования составило 200 мг/л при постоянном поверхностном натяжении 39 мН/м. Новый штамм Gordonia amicalis G2 продемонстрировал эффективную способность к продукции гликолипидных биосурфактантов. Учитывая способность Gordonia amicalis G2 участвовать в синтезе каротиноидов, можно предположить разработку комплексной технологии получения вторичных метаболитов на основе данного штамма.

The present study was aimed at analyzing the ability of a new paraffin-degrading strain, Gordonia amicalis G2, isolated from the contaminated sand of a seaport in Vietnam, to produce biosurfactants. The production of biosurfactants by the Gordonia amicalis G2 strain was monitored during bacterial growth in batch culture with the use of hexadecane as the growth medium. The effectiveness of produced biosurfactants was evaluated in terms of surface and interfacial tension. The surface and interfacial tension of biosurfactant solutions were determined using the du Noüy ring method by means of a Kruss K6 tensiometer. Surface tension decreased to 39 mN/m; interfacial tension, to 2 mN/m. The chemical structure of biosurfactants was characterized using thin-layer chromatography and Fourier transform infrared spectroscopy. A qualitative assessment revealed the glycolipid nature of secondary metabolites. The critical micelle concentration was determined from the inflection point of the curves showing the dependence of surface tension on the biosurfactant content. The critical micelle concentration amounted to 200 mg/L at a constant surface tension of 39 mN/m. The new Gordonia amicalis G2 strain was found to have an effective ability to produce glycolipid biosurfactants. Given the ability of Gordonia amicalis G2 to participate in the synthesis of carotenoids, an integrated technology could be developed for secondary metabolite production by this strain.

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The authors declare that there are no conflicts of interest present.