<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vuzbiochemi</journal-id><journal-title-group><journal-title xml:lang="ru">Известия вузов. Прикладная химия и биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of Universities. Applied Chemistry and Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-2925</issn><issn pub-type="epub">2500-1558</issn><publisher><publisher-name>ИРНИТУ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21285/achb.996</article-id><article-id custom-type="edn" pub-id-type="custom">BUWBKE</article-id><article-id custom-type="elpub" pub-id-type="custom">vuzbiochemi-1521</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PHYSICOCHEMICAL BIOLOGY</subject></subj-group></article-categories><title-group><article-title>Влияние состава среды на рост мицелия и биосинтез метаболитов Daedaleopsis tricolor</article-title><trans-title-group xml:lang="en"><trans-title>Culture medium composition influences mycelial growth and metabolite biosynthesis of Daedaleopsis tricolor</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4228-9124</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сысоева</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Sysoeva</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сысоева Мария Александровна, д.х.н., профессор</p><p>420015, г. Казань, ул. Карла Маркса, 68</p></bio><bio xml:lang="en"><p>Maria A. Sysoeva, Dr. Sci. (Chemistry), Professor</p><p>68, Karl Marx St., Kazan, 420015</p></bio><email xlink:type="simple">oxygen1130@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6661-6217</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Прозорова</surname><given-names>И. Ш.</given-names></name><name name-style="western" xml:lang="en"><surname>Prozorova</surname><given-names>I. Sh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Прозорова Илюза Шамилевна, ассистент</p><p>420015, г. Казань, ул. Карла Маркса, 68</p></bio><bio xml:lang="en"><p>Ilyuza Sh. Prozorova, Assistant</p><p>68, Karl Marx St., Kazan, 420015</p></bio><email xlink:type="simple">kuleeva.1996@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0592-3667</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сысоева</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Sysoeva</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сысоева Елена Владиславовна, к.х.н., доцент</p><p>420015, г. Казань, ул. Карла Маркса, 68</p></bio><bio xml:lang="en"><p>Elena V. Sysoeva, Cand. Sci. (Chemistry), Associate Professor</p><p>68, Karl Marx St., Kazan, 420015</p></bio><email xlink:type="simple">inonotus@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Казанский национальный исследовательский технологический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Kazan National Research Technological University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>07</day><month>10</month><year>2025</year></pub-date><volume>15</volume><issue>3</issue><fpage>385</fpage><lpage>394</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Сысоева М.А., Прозорова И.Ш., Сысоева Е.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Сысоева М.А., Прозорова И.Ш., Сысоева Е.В.</copyright-holder><copyright-holder xml:lang="en">Sysoeva M.A., Prozorova I.S., Sysoeva E.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vuzbiochemi.elpub.ru/jour/article/view/1521">https://vuzbiochemi.elpub.ru/jour/article/view/1521</self-uri><abstract><p>Метаболиты Daedaleopsis tricolor представляют практический интерес, поскольку они обладают антиоксидантными, антимикробными, противоопухолевыми свойствами. Погруженное культивирование Daedaleopsis tricolor KS11 было осуществлено на питательных средах, содержащих разные источники азота – дрожжевой экстракт, соевый изолят и их комбинацию в соотношении 1:1. Было показано, что культивирование Daedaleopsis tricolor KS11 на комбинированной среде позволяет получить до 9 г/л биомассы. При этом на 1 сутки сокращается продолжительность его выращивания по сравнению с использованием дрожжевого экстракта или соевого изолята в качестве единственных источников азота. При культивировании базидиомицета на комбинированной среде суммарные экзометаболиты проявляют высокие антиоксидантные (88,61 мг/г) и антирадикальные (3,41 мг/мл) свойства. Это коррелирует с высоким содержанием в них флавоноидов – 18,56 мг/г по сравнению с экзометаболитами, полученными при культивировании гриба на других средах. Показано, что для получения эндопигментов эффективнее использовать в качестве источника азота соевый изолят. На основании инфракрасного спектрометрического анализа эндопигменты отнесены к меланинам. Установлено, что перспективно культивировать Daedaleopsis tricolor KS11 на комбинированной среде, содержащей дрожжевой экстракт и соевый изолят в соотношении 1:1, поскольку на ней гриб накапливает экзо- и эндопигменты с высокими антиоксидантными свойствами. Полученные результаты можно использовать для создания биологически активных добавок антиоксидантного действия.</p></abstract><trans-abstract xml:lang="en"><p>The metabolites of the basidiomycete Daedaleopsis tricolor have attracted research attention due to their antioxidant, antimicrobial, and antitumor properties. This study investigated the submerged cultivation of the Daedaleopsis tricolor strain KS11 in nutrient media supplemented with varying nitrogen sources, including yeast extract, soy protein isolate, and a 1:1 combination thereof. The cultivation on the combined medium yielded up to 9 g/L of biomass and reduced the cultivation time by one day compared to media containing a single nitrogen source. The total pool of exometabolites obtained using the combined medium demonstrated high antioxidant (88.61 mg/g) and antiradical activity (3.41 mg/mL). This activity correlated with a high flavonoid content of 18.56 mg/g, which exceeded the levels found in exometabolites from the other media. The use of soy protein isolate as a nitrogen source was more effective for the synthesis of endopigments. These endopigments were identified as melanins by infrared spectrometry. The cultivation of Daedaleopsis tricolor KS11 on a combined medium containing a 1:1 ratio of yeast extract and soy protein isolate represents a promising approach, as it facilitates the concurrent accumulation of exopigments and endopigments with high antioxidant properties. These findings provide a basis for developing dietary supplements with enhanced antioxidant activity.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Daedaleopsis tricolor</kwd><kwd>погруженное культивирование</kwd><kwd>эндопигменты</kwd><kwd>флавоноиды</kwd><kwd>антиоксидантная активность</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Daedaleopsis tricolor</kwd><kwd>submerged cultivation</kwd><kwd>endopigments</kwd><kwd>flavonoids</kwd><kwd>antioxidant activity</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Mišković J., Rašeta M., Čapelja E., Krsmanović N., Novaković A., Karaman M. Mushroom species Stereum hirsutum as natural source of phenolics and fatty acids as antioxidants and acetylcholinesterase inhibitors // Chemistry &amp; Biodiversity. 2021. Vol. 18. N 11. P. e2100409. DOI: 10.1002/cbdv.202100409.</mixed-citation><mixed-citation xml:lang="en">Mišković J., Rašeta M., Čapelja E., Krsmanović N., Novaković A., Karaman M. Mushroom species Stereum hirsutum as natural source of phenolics and fatty acids as antioxidants and acetylcholinesterase inhibitors. Chemistry &amp; Biodiversity. 2021;18(11):e2100409. DOI: 10.1002/cbdv.202100409.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Karaman M., Čapelja E., Rašeta M., Rakić M. Diversity, chemistry, and environmental contamination of wild growing medicinal mushroom species as sources of biologically active substances (antioxidants, anti-diabetics, and ache inhibitors) // Biology, Cultivation and Applications of Mushrooms / eds A. Arya, K. Rusevska. Singapore: Springer, 2022. P. 203–257. DOI: 10.1007/978-981-16-6257-7_8.</mixed-citation><mixed-citation xml:lang="en">Karaman M., Čapelja E., Rašeta M., Rakić M. Diversity, chemistry, and environmental contamination of wild growing medicinal mushroom species as sources of biologically active substances (antioxidants, antidiabetics, and ache inhibitors). In: Arya A., Rusevska K. (eds). Biology, Cultivation and Applications of Mushrooms. Singapore: Springer; 2022, p. 203-257. DOI: 10.1007/978-981-16-6257-7_8.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Gariboldi M.B., Marras E., Ferrario N., Vivona V., Prini P., Vignati F., et al. Anti-cancer potential of edible/ medicinal mushrooms in breast cancer // International Journal of Molecular Sciences. 2023. Vol. 24, no. 12. P. 10120. DOI: 10.3390/ijms241210120.</mixed-citation><mixed-citation xml:lang="en">Gariboldi M.B., Marras E., Ferrario N., Vivona V., Prini P., Vignati F., et al. Anti-cancer potential of edible/medicinal mushrooms in breast cancer. International Journal of Molecular Sciences. 2023;24(12):10120. DOI: 10.3390/ijms241210120.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Bang T.H., Suhara H., Dоі K., Ishikawa H., Fukami K., Parajuli G.P., et al. Wild mushrooms in Nepal: some potential candidates as antioxidant and ACE-inhibition sources // Evidence-Based Complementary and Alternative Medicine. 2014. P. 95305. DOI: 10.1155/2014/195305.</mixed-citation><mixed-citation xml:lang="en">Bang T.H., Suhara H., Dоі K., Ishikawa H., Fukami K., Parajuli G.P., et al. Wild mushrooms in Nepal: some potential candidates as antioxidant and ACE-inhibition sources. Evidence-Based Complementary and Alternative Medicine. 2014:95305. DOI: 10.1155/2014/195305.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Rašeta M., Miškovic J., Čapelja E., Zapora E., Fabijan A.P., Kneževi´c P., et al. Do Ganoderma species represent novel sources of phenolic based antimicrobial agents? // Molecules. 2023. Vol. 28, no. 7. P. 3264. DOI: 10.3390/molecules28073264.</mixed-citation><mixed-citation xml:lang="en">Rašeta M., Miškovic J., Čapelja E., Zapora E., Fabijan A.P., Kneževi´c P., et al. Do Ganoderma species represent novel sources of phenolic based antimicrobial agents? Molecules. 2023;28(7):3264. DOI: 10.3390/molecules28073264.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Adhikari M., Bhusal S., Pandey M.R., Raut J.K., Bhatt L.R. Mycochemical and nutritional analysis of selected wild mushrooms from Gaurishankar conservation area, Nepal // International Journal of Pharmacognosy and Chinese Medicine. 2019. Vol. 3, no. 3. P. 000169. DOI: 10.23880/ipcm-16000169.</mixed-citation><mixed-citation xml:lang="en">Adhikari M., Bhusal S., Pandey M.R., Raut J.K., Bhatt L.R. Mycochemical and nutritional analysis of selected wild mushrooms from Gaurishankar conservation area, Nepal. International Journal of Pharmacognosy and Chinese Medicine. 2019;3(3):000169. DOI: 10.23880/ipcm-16000169.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Kurchenko V.P., Sushinskaya N.V., Kiseleva I.S., Ermoshin A.A. Biologically active substances in fruit bodies of wood decomposing fungi // AIP Conference Proceedings. 2022. Vol. 2390, no. 1. P. 030045. DOI: 10.1063/5.0069253.</mixed-citation><mixed-citation xml:lang="en">Kurchenko V.P., Sushinskaya N.V., Kiseleva I.S., Ermoshin A.A. Biologically active substances in fruit bodies of wood decomposing fungi. AIP Conference Proceedings. 2022;2390(1):030045. DOI: 10.1063/5.0069253.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Ставский Е.А., Теплякова Т.В., Андреева И.С., Давыдова Е.С., Ставская А.А., Потешкина А.Л. Экспериментальная оценка лечебных свойств мази на основе меланинов из природного сырья и глубинной культуры чаги // Journal of Siberian Medical Sciences. 2022. Т. 6. N 1. С. 93–105. DOI: 10.31549/2542-1174-2022-6-1-93-105. EDN: GNWRSX.</mixed-citation><mixed-citation xml:lang="en">Stavsky E.A., Teplyakova T.V., Andreeva I.S., Davydova E.S., Stavskaya A.A., Poteshkina A.L. Experimental evaluation of therapeutic properties of ointment based on melanins from natural raw material and a submerged culture of chaga (Inonotus оbliquus). Journal of Siberian Medical Sciences. 2022;6(1):93-105. (In Russian). DOI: 10.31549/2542-1174-2022-6-1-93-105. EDN: GNWRSX.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Kanehara R., Tonouchi A., Konno K., Hashimoto M. Cyclohumulanoid sesquiterpenes from the culture broth of the basidiomycetous fungus Daedaleopsis tricolor // Molecules. 2021. Vol. 26, no. 14. P. 4364. DOI: 10.3390/molecules26144364.</mixed-citation><mixed-citation xml:lang="en">Kanehara R., Tonouchi A., Konno K., Hashimoto M. Cyclohumulanoid sesquiterpenes from the culture broth of the basidiomycetous fungus Daedaleopsis tricolor. Molecules. 2021;26(14):4364. DOI: 10.3390/molecules26144364.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Zhao J.-Y., Feng T., Li Z.-H., Dong Z.-J., Zhang H.-B., Liu J.-K. Sesquiterpenoids and an ergosterol from cultures of the fungus Daedaleopsis tricolor // Natural Products and Bioprospecting. 2013. Vol. 3. P. 271–276. DOI: 10.1007/s13659-013-0065-0.</mixed-citation><mixed-citation xml:lang="en">Zhao J.-Y., Feng T., Li Z.-H., Dong Z.-J., Zhang H.-B., Liu J.-K. Sesquiterpenoids and an ergosterol from cultures of the fungus Daedaleopsis tricolor. Natural Products and Bioprospecting. 2013;3:271-276. DOI: 10.1007/s13659-013-0065-0.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Kim E.-M., Jung H.-R., Min T.-J. Purification, structure determination and biological activities of 20(29)-lupen-3-one from Daedaleopsis tricolor (Bull. ex Fr.) Bond. et Sing // Bulletin of the Korean Chemical Society. 2001. Vol. 22, no. 1. P. 59–62.</mixed-citation><mixed-citation xml:lang="en">Kim E.-M., Jung H.-R., Min T.-J. Purification, structure determination and biological activities of 20(29)-lupen-3-one from Daedaleopsis tricolor (Bull. ex Fr.) Bond. et Sing. Bulletin of the Korean Chemical Society. 2001;22(1):59-62.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Kanehara R., Tonouchi A., Konno K., Koshino H., Hashimoto M. Isolation of cyclohumulanoids from Daedaleopsis tricolor and their biosynthesis based on in silico simulations // Tetrahedron. 2022. Vol. 123. P. 133006. DOI: 10.1016/j.tet.2022.133006.</mixed-citation><mixed-citation xml:lang="en">Kanehara R., Tonouchi A., Konno K., Koshino H., Hashimoto M. Isolation of cyclohumulanoids from Daedaleopsis tricolor and their biosynthesis based on in silico simulations. Tetrahedron. 2022;123:133006. DOI: 10.1016/j.tet.2022.133006.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Ćilerdžić J., Stajić M.M., Milovanović I.N., Galić M.M., Vukojević J.B. Antioxidative potential of Daedaleopsis tricolor basidiocarps and mycelium // Zbornik Matice srpske za prirodne nauke. 2017. Vol. 132. P. 19–27. DOI: 10.2298/ZMSPN1732019C.</mixed-citation><mixed-citation xml:lang="en">Ćilerdžić J., Stajić M.M., Milovanović I.N., Galić M.M., Vukojević J.B. Antioxidative potential of Daedaleopsis tricolor basidiocarps and mycelium. Zbornik Matice srpske za prirodne nauke. 2017;132:19-27. DOI: 10.2298/ZMSPN1732019C.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Проценко М.А., Костина Н.Е., Теплякова Т.В. Подбор питательных сред для глубинного культивирования дереворазрушающего гриба Daedaleopsis tricolor (Bull.) Bondartsev et Singer // Биотехнология. 2018. Т. 34. N 1. С. 45–51. DOI: 10.21519/0234-2758-2018-34-1-45-51. EDN: YWQNGB.</mixed-citation><mixed-citation xml:lang="en">Protsenko M.A., Kostina N.E., Teplyakova T.V. Selection of nutrient media for submerged culturing of wood-destroying mushroom of Daedaleopsis tricolor (Bull.) Bondartsev et Singer. Biotehnologia. 2018;34(1):45-51. (In Russian). DOI: 10.21519/0234-2758-2018-34-1-45-51. EDN: YWQNGB.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Сысоева Е.В., Прозорова И.Ш., Сысоева М.А., Парикова Ю.С. Метаболиты, синтезируемые Рycnoporellus fulgens KS12 при погруженном культивировании // Бутлеровские сообщения. 2024. Т. 80. N 12. C. 175–184. DOI: 10.37952/ROI-jbc-01/24-80-12-175. EDN: DWCTTP.</mixed-citation><mixed-citation xml:lang="en">Sysoeva E.V., Prozorova I.S., Sysoeva M.A., Parikova Y.S. Metabolites produced by the Pycnoporellus fulgens KS12 during submerged cultivation. Butlerov Communications. 2024;80(12):175-184. (In Russian). DOI: 10.37952/ROI-jbc-01/24-80-12-175. EDN: DWCTTP.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Хабибрахманова В.Р., Рассабина А.Е., Хайруллина А.Ф., Минибаева Ф.В. Физико-химические характеристики и антиоксидантные свойства меланинов, выделенных из лишайника Leptogium furfuraceum (Harm.) // Химия растительного сырья. 2022. N 4. С. 115–125. DOI: 10.14258/jcprm.20220411774. EDN: SOUXVT.</mixed-citation><mixed-citation xml:lang="en">Khabibrakhmanova V.R., Rassabina A.E., Khayrullina A.F., Minibayeva F.V. Physico-chemical characteristics and antioxidant properties of melanins extracted from Leptogium furfuraceum (Harm.). Chemistry of plant raw material. 2022;4:115-125. (In Russian). DOI: 10.14258/jcprm.20220411774. EDN: SOUXVT.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Saadaoui N., Mathlouthi A., Zaiter A., El-Bok S., Mokni M., Harbi M., et al. Phytochemical profiling, antioxidant potential and protective effect of leaves extract of tunisian Vitis vinifera autochthonous accessions against acute CCl4-injured hepatotoxicity in mice // Heliyon. 2023. Vol. 9, no. 5. P. e16377. DOI: 10.1016/j.heliyon.2023.e16377.</mixed-citation><mixed-citation xml:lang="en">Saadaoui N., Mathlouthi A., Zaiter A., El-Bok S., Mokni M., Harbi M., et al. Phytochemical profiling, antioxidant potential and protective effect of leaves extract of tunisian Vitis vinifera autochthonous accessions against acute CCl4-injured hepatotoxicity in mice. Heliyon. 2023;9(5):e16377. DOI: 10.1016/j.heliyon.2023.e16377.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Klyushova L.S., Kandalintseva N.V., Grishanova A.Y. Antioxidant activity of new sulphur- and selenium-containing analogues of potassium phenosan against H 2 O 2 -induced cytotoxicity in tumour cells // Current Issues in Molecular Biology. 2022. Vol. 44, no. 7. P. 3131–3145. DOI: 10.3390/cimb44070216.</mixed-citation><mixed-citation xml:lang="en">Klyushova L.S., Kandalintseva N.V., Grishanova A.Y. Antioxidant activity of new sulphur- and selenium-containing analogues of potassium phenosan against H2O2-induced cytotoxicity in tumour cells. Current Issues in Molecular Biology. 2022;44(7):3131-3145. DOI: 10.3390/cimb44070216.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Kannan J., Pang K.-L., Ho Y.-N., Hsu P.-H., Chen L.-L. A comparison of the antioxidant potential and metabolite analysis of marine fungi associated with the red algae Pterocladiella capillacea from Northern Taiwan // Antioxidants. 2024. Vol. 13, no. 3. P. 336. DOI: 10.3390/antiox13030336.</mixed-citation><mixed-citation xml:lang="en">Kannan J., Pang K.-L., Ho Y.-N., Hsu P.-H., Chen L.-L. A comparison of the antioxidant potential and metabolite analysis of marine fungi associated with the red algae Pterocladiella capillacea from Northern Taiwan. Antioxidants. 2024;13(3):336. DOI: 10.3390/antiox13030336.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Ribera J., Panzarasa G., Stobbe A., Osypova A., Rupper P., Klose D., Schwarze F.W.M.R. Scalable Biosynthesis of Melanin by the Basidiomycete Armillaria cepistipes // Journal of Agricultural and Food Chemistry. 2019. Vol. 67, no. 1. P. 132–139. DOI: 10.1021/acs.jafc.8b05071.</mixed-citation><mixed-citation xml:lang="en">Ribera J., Panzarasa G., Stobbe A., Osypova A., Rupper P., Klose D., Schwarze F.W.M.R. Scalable Biosynthesis of Melanin by the Basidiomycete Armillaria cepistipes. Journal of Agricultural and Food Chemistry. 2019;67(1):132-139. DOI: 10.1021/acs.jafc.8b05071.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Mišković J., Karaman M., Rašeta M., Krsmanović N., Berežni S., Jakovljević D., et al. Comparison of two Schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation // Journal of Fungi. 2021. Vol. 7, no. 2. P. 115. DOI: 10.3390/jof7020115.</mixed-citation><mixed-citation xml:lang="en">Mišković J., Karaman M., Rašeta M., Krsmanović N., Berežni S., Jakovljević D., et al. Comparison of two Schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation. Journal of Fungi. 2021;7(2):115. DOI: 10.3390/jof7020115.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Krupodorova T., Barshteyn V., Dzhagan V., Pluzhnyk A., Zaichenko T., Blume Ya. Enhancement of antioxidant activity and total phenolic content of Fomitopsis pinicola mycelium extract // Fungal Biology and Biotechnology. 2024. Vol. 11. P. 18. DOI: 10.1186/s40694-024-00187-0.</mixed-citation><mixed-citation xml:lang="en">Krupodorova T., Barshteyn V., Dzhagan V., Pluzhnyk A., Zaichenko T., Blume Ya. Enhancement of antioxidant activity and total phenolic content of Fomitopsis pinicola mycelium extract. Fungal Biology and Biotechnology. 2024;11:18. DOI: 10.1186/s40694-024-00187-0.</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Krupodorova T., Barshteyn V., Gafforov Yu., Raseta M., Zaichenko T., Blume Ya. Comparative evaluation of free radical scavenging activity and total metabolite profiles among 30 macrofungi species // Bioresources and Bioprocessing. 2025. Vol. 12. P. 13. DOI: 10.1186/s40643-025-00841-4.</mixed-citation><mixed-citation xml:lang="en">Krupodorova T., Barshteyn V., Gafforov Yu., Raseta M., Zaichenko T., Blume Ya. Comparative evaluation of free radical scavenging activity and total metabolite profiles among 30 macrofungi species. Bioresources and Bioprocessing. 2025;12:13. DOI: 10.1186/s40643-025-00841-4.</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Chutimanukul P., Sukdee S., Prajuabjinda O., Thepsilvisut O., Panthong S., Athinuwat D., et al. The effects of soybean meal on growth, bioactive compounds, and antioxidant activity of Hericium erinaceus // Horticulturae. 2023. Vol. 9, no. 6. P. 693. DOI: 10.3390/horticulturae9060693.</mixed-citation><mixed-citation xml:lang="en">Chutimanukul P., Sukdee S., Prajuabjinda O., Thepsilvisut O., Panthong S., Athinuwat D., et al. The effects of soybean meal on growth, bioactive compounds, and antioxidant activity of Hericium erinaceus. Horticulturae. 2023;9(6):693. DOI: 10.3390/horticulturae9060693.</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Прозорова И.Ш., Сысоева М.А., Сысоева Е.В., Красильников Р.О. Антиоксидантная активность культуральной жидкости Daedaleopsis tricolor KS11, содержание в ней флавоноидов, простых фенолов // BIOAsia-Altai 2024: материалы IV Междунар. биотехнологического форума (г. Барнаул, 23–28 сентября 2024 г.). Барнаул: Изд-во АлтГУ, 2024. С. 169–172.</mixed-citation><mixed-citation xml:lang="en">Prozorova I.Sh., Sysoeva M.A., Sysoeva E.V., Krasilnikov R.O. Antioxidant activity of Daedaleopsis tricolor KS11 culture liquid, its content of flavonoids, simple phenols. In: BIOAsia-Altai 2024: materialy IV Mezhdunar. biotekhnologicheskogo foruma = BIOAsia-Altai 2024: Proc. IV Int. Biotech. Forum. 23–28 September 2024, Barnaul. Barnaul: Altai State University; 2024, p. 169-172. (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Sadowska-Bartosz I., Bartosz G. Evaluation of the antioxidant capacity of food products: methods, applications and limitations // Processes. 2022. Vol. 10, no. 10. P. 2031. DOI: 10.3390/pr10102031.</mixed-citation><mixed-citation xml:lang="en">Sadowska-Bartosz I., Bartosz G. Evaluation of the antioxidant capacity of food products: methods, applications and limitations. Processes. 2022;10(10):2031. DOI: 10.3390/pr10102031.</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Souilem F., Fernandes A., Calhelha R.C., Barreira J.C.M., Barros L., Skhiri F., et al. Wild mushrooms and their mycelia as sources of bioactive compounds: antioxidant, anti-inflammatory and cytotoxic properties // Food Chemistry. 2017. Vol. 230. P. 40–48. DOI: 10.1016/j.foodchem.2017.03.02.</mixed-citation><mixed-citation xml:lang="en">Souilem F., Fernandes A., Calhelha R.C., Barreira J.C.M., Barros L., Skhiri F., et al. Wild mushrooms and their mycelia as sources of bioactive compounds: antioxidant, anti-inflammatory and cytotoxic properties. Food Chemistry. 2017;230:40-48. DOI: 10.1016/j.foodchem.2017.03.02.</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Le T.N., Tran N.T.H., Pham V.N.T., Van-Thi N.-D., Tran H.T.M. Anti-ultraviolet, antibacterial, and biofilm eradication activities against Cutibacterium acnes of melanins and melanin derivatives from Daedaleopsis tricolor and Fomes fomentarius // Frontiers in Microbiology. 2023. Vol. 14. P. 1305778. DOI: 10.3389/fmicb.2023.1305778.</mixed-citation><mixed-citation xml:lang="en">Le T.N., Tran N.T.H., Pham V.N.T., Van-Thi N.-D., Tran H.T.M. Anti-ultraviolet, antibacterial, and biofilm eradication activities against Cutibacterium acnes of melanins and melanin derivatives from Daedaleopsis tricolor and Fomes fomentarius. Frontiers in Microbiology. 2023;14:1305778. DOI: 10.3389/fmicb.2023.1305778.</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Pralea I.-E. Moldovan R.-C., Petrache A.-M., Ilie-M., Heghe-S.-C., Ielciu I., et al. From extraction to advanced analytical methods: the challenges of melanin analysis // International Journal of Molecular Sciences. 2019. Vol. 20, no. 16. P. 3943. DOI: 10.3390/ijms20163943.</mixed-citation><mixed-citation xml:lang="en">Pralea I.-E. Moldovan R.-C., Petrache A.-M., Ilie-M., Heghe-S.-C., Ielciu I., et al. From extraction to advanced analytical methods: the challenges of melanin analysis. International Journal of Molecular Sciences. 2019;20(16):3943. DOI: 10.3390/ijms20163943.</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Wang L.-F., Rhim J.-W. Isolation and characterization of melanin from black garlic and sepia ink // LWT – Food Science and Technology. 2019. Vol. 99. P. 17–23. DOI: 10.1016/j.lwt.2018.09.033.</mixed-citation><mixed-citation xml:lang="en">Wang L.-F., Rhim J.-W. Isolation and characterization of melanin from black garlic and sepia ink. LWT – Food Science and Technology. 2019;99:17-23. DOI: 10.1016/j.lwt.2018.09.033.</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Nguyen H.A.T., Ho T.Ph., Mangelings D., Eeckhaut A.V., Heyden Y.V., Tran H.T.M. Antioxidant, neuroprotective, and neuroblastoma cells (SH-SY5Y) differentiation effects of melanins and arginine-modified melanins from Daedaleopsis tricolor and Fomes fomentarius // BMC Biotechnology. 2024. Vol. 24. P. 89. DOI: 10.1186/s12896-024-00918-6.</mixed-citation><mixed-citation xml:lang="en">Nguyen H.A.T., Ho T.Ph., Mangelings D., Eeckhaut A.V., Heyden Y.V., Tran H.T.M. Antioxidant, neuroprotective, and neuroblastoma cells (SH-SY5Y) differentiation effects of melanins and arginine-modified melanins from Daedaleopsis tricolor and Fomes fomentarius. BMC Biotechnology. 2024;24:89. DOI: 10.1186/s12896-024-00918-6.</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Hou R., Liu X., Xiang K., Chen L., Wu X., Lin W., et al. Characterization of the physicochemical properties and extraction optimization of natural melanin from Inonotus hispidus mushroom // Food Chemistry. 2019. Vol. 277. P. 533–542. DOI: 10.1016/j.foodchem.2018.11.002.</mixed-citation><mixed-citation xml:lang="en">Hou R., Liu X., Xiang K., Chen L., Wu X., Lin W., et al. Characterization of the physicochemical properties and extraction optimization of natural melanin from Inonotus hispidus mushroom. Food Chemistry. 2019;277:533-542. DOI: 10.1016/j.foodchem.2018.11.002.</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Ye M., Guo G., Lu Yi., Song Sh., Wang H.-Y., Yang L. Purification, structure and anti-radiation activity of melanin from Lachnum YM404 // International Journal of Biological Macromolecules. 2014. Vol. 63. P. 170–176. DOI: 10.1016/j.ijbiomac.2013.10.046.</mixed-citation><mixed-citation xml:lang="en">Ye M., Guo G., Lu Yi., Song Sh., Wang H.-Y., Yang L. Purification, structure and anti-radiation activity of melanin from Lachnum YM404. International Journal of Biological Macromolecules. 2014;63:170-176. DOI: 10.1016/wj.ijbiomac.2013.10.046.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
