vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2019-9-3-420-429vuzbiochemi-214Research ArticleФИЗИКО-ХИМИЧЕСКАЯ И ОБЩАЯ БИОЛОГИЯPHYSICOCHEMICAL AND GENERAL BIOLOGYИсследование влияния дозировки инокулята Medusomyces gisevii Sa-12 на выход и степень полимеризации бактериальной целлюлозыStudy of the influence of Medusomyces gisevii Sa-12 inoculum dosage on bacterial cellulose yield and degree of polymerisationСкибаЕ. А.SkibaE. A.

к.т.н., доцент, старший научный сотрудник лаборатории биоконверсии,

г. Бийск

Ph.D. (Engineering), Associate Professor, Senior Researcher, Laboratory of Bioconversion,

Biysk

eas08988@mail.ruБайбаковаО. В.BaibakovaO. V.

к.т.н., младший научный сотрудник лаборатории биоконверсии,

г. Бийск

Ph.D. (Engineering), Junior Researcher, Laboratory of Bioconversion,

Biysk

olka_baibakova@mail.ruГладышеваЕ. К.GladyshevaE. K.

к.т.н., научный сотрудник лаборатории биоконверсии,

г. Бийск

Ph.D. (Engineering), Researcher, Laboratory of Bioconversion,

Biysk

evg-gladysheva@yandex.ruБудаеваВ. В.BudaevaV. V.

к.х.н., доцент, заведующая лабораторией биоконверсии,

г. Бийск

Ph.D. (Chemistry), Associate Professor, Head of Bioconversion Laboratory,

Biysk

budaeva@ipcet.ruИнститут проблем химико-энергетических технологий СО РАНРоссияInstitute for Problems of Chemical and Energetic Technologies of the SB RASRussian Federation20190710201993420429Copyright © Скиба Е.А., Байбакова О.В., Гладышева Е.К., Будаева В.В., 20192019Скиба Е.А., Байбакова О.В., Гладышева Е.К., Будаева В.В.Skiba E.A., Baibakova O.V., Gladysheva E.K., Budaeva V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/214

Бактериальная целлюлоза (БЦ) является высококристаллическим нанополимером с уникальными физико-механическими свойствами, поэтому обладает превосходным потенциалом применения как в традиционных, так и в новых отраслях. В масштабированных процессах биосинтеза БЦ перспективно применение консорциумов микроорганизмов, характеризующихся адаптивностью и обладающих синергетическими эффектами в координирующем потреблении субстрата, поэтому в данной работе используется симбиотическая культура Мedusomyces gisevii Sa-12. В работе подробно исследуется влияние дозировки инокулята на выход БЦ при культивировании на глюкозной среде в оптимальных условиях. Для контроля качества БЦ выбрано два доступных метода: растровая электронная микроскопия, которая является экспресс-методом подтверждения происхождения целлюлозы и степень полимеризации, как распространённый метод контроля качества целлюлозы. Проведено четыре эксперимента с внесением продуцента c дозировкой 5% об., 10% об., 15% об. и 20% об. Выявлено что, при использовании симбиотической культуры Мedusomyces gisevii Sa-12 наибольшая численность уксуснокислых бактерий и наибольший выход БЦ (7,5–8,0%) обеспечиваются при дозировке инокулята 10–20% об. При этом дозировка инокулята 20% об. позволяет сократить продолжительность культивирования вдвое. Дозировка инокулята 5% об. является недостаточной. Показано, что все варианты дозировки инокулята обеспечивают одинаковое трехмерное сетчатое микрофибриллярное строение образцов БЦ. Впервые установлено, что степень полимеризации (СП) образцов БЦ зависит от дозировки инокулята и продолжительности биосинтеза БЦ. Таким образом, с помощью простого параметра – дозировки инокулята – можно управлять процессом биосинтеза и направленно синтезировать БЦ с заданной СП. Установлено, что дозировка инокулята 10% об., обеспечивает получение максимально высокой СП БЦ – 5000, которая при длительном культивировании снижается незначительно.

Bacterial cellulose (BC) consists in a highly crystalline nanopolymer whose potential for application in both traditional and new industries is significant due to its unique physico-mechanical properties. In scaled BC biosynthesis processes, the use of microorganism consortia characterised by their adaptability and synergistic effects when coordinating substrate consumption appears to be promising. In the present work, the effect of inoculum dosage on BC yield during Medusomyces gisevii Sa-12 symbiotic culture on a glucose medium under optimal conditions is studied in detail. Two available methods were chosen for quality control of the BC: scanning electron microscopy, presenting an express method for confirming the origin of cellulose; and the degree of polymerisation in terms of a common method for controlling the quality of cellulose. Four experiments were carried out for the producer introduction with a dosage of 5, 10, 15 and 20% vol.. Use of the Medusomyces gisevii Sa-12 symbiotic culture provided the greatest number of acetic acid bacteria and the highest BC yield (7.5–8.0%) with an inoculum dosage of 10–20% vol. At the same time, an inoculum dosage of 20% vol. allowed the culture time to be halved, while an inoculum dosage of 5% vol. appears to be insufficient. All inoculum dosage variants were determined to provide the same three-dimensional cross-linked microfibrillar structure of BC samples. The degree of polymerisation (DP) of BC samples was first established to depend on the dosage of the inoculum and the duration of BC biosynthesis. Thus, the biosynthesis process can be controlled using such a simple parameter as inoculum dosage and BCs can be synthesised directionally with a given DP. The inoculum dosage of 10% vol. was established as providing the highest possible DP of BC (value of 5000), decreasing slightly during prolonged culture.

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The authors declare that there are no conflicts of interest present.