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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vuzbiochemi</journal-id><journal-title-group><journal-title xml:lang="ru">Известия вузов. Прикладная химия и биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of Universities. Applied Chemistry and Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-2925</issn><issn pub-type="epub">2500-1558</issn><publisher><publisher-name>ИРНИТУ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21285/2227-2925-2019-9-3-500-508</article-id><article-id custom-type="elpub" pub-id-type="custom">vuzbiochemi-221</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ФИЗИКО-ХИМИЧЕСКАЯ И ОБЩАЯ БИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PHYSICOCHEMICAL AND GENERAL BIOLOGY</subject></subj-group></article-categories><title-group><article-title>Повышение биологической ценности белка и увеличение сроков хранения полуфабриката из вешенки обыкновенной методом электронной стерилизации</article-title><trans-title-group xml:lang="en"><trans-title>Use of electronic sterilisation to increase the biological protein value and increase the shelf life of semi-finished oyster mushroom products</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дриль</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Dril</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>старший преподаватель кафедры технологии и организации пищевых производств,</p><p>г. Новосибирск</p></bio><bio xml:lang="en"><p>Senior Lecturer, Technology and Organization of Food Industries Department,</p><p>Novosibirsk</p></bio><email xlink:type="simple">drilnaska@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рождественская</surname><given-names>Л. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Rozhdestvenskaya</surname><given-names>L. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.э.н., доцент, заведующая кафедрой технологии и организации пищевых производств,</p><p>г. Новосибирск</p></bio><bio xml:lang="en"><p>Ph.D. (Economics), Associate Professor, Head of Technology and Organization of Food Industries Department,</p><p>Novosibirsk</p></bio><email xlink:type="simple">lada2006job@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Новосибирский государственный технический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Novosibirsk State Technical University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>08</day><month>10</month><year>2019</year></pub-date><volume>9</volume><issue>3</issue><fpage>500</fpage><lpage>508</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Дриль А.А., Рождественская Л.Н., 2019</copyright-statement><copyright-year>2019</copyright-year><copyright-holder xml:lang="ru">Дриль А.А., Рождественская Л.Н.</copyright-holder><copyright-holder xml:lang="en">Dril A.A., Rozhdestvenskaya L.N.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vuzbiochemi.elpub.ru/jour/article/view/221">https://vuzbiochemi.elpub.ru/jour/article/view/221</self-uri><abstract><p>Вопрос биологической ценности белка грибов не ограничивается только изучением его состава. Он связан с проблемой его перевариваемости и усвояемости и широко обсуждается в научных кругах, в том числе в научных публикациях, рассмотренных в данной статье. В зарубежных странах распространено использование микопротеина на основе биомассы одноклеточных грибков, богатых протеином. В России в качестве альтернативного источника белка используются высшие грибы, в том числе вешенка обыкновенная (Pleurotus ostreatus). Известно, что грибные белки при богатом аминокислотном составе усваиваются на уровне растительных белков, так как они «упакованы» в межклеточную хитиновую оболочку. Для разрушения оболочки с целью высвобождения белковой макромолекулы необходима обработка грибного сырья, проводимая различными технологическими способами. Существуют традиционные способы обработки, такие как измельчение, термическое воздействие и др. В данной же статье рассматривается электронная стерилизация полуфабрикатов как средство повышения безопасности продукта. Авторами изучено влияние ионизирующего электронного излучения на физико-химические свойства грибной продукции на примере термообработанного вакуумированного полуфабриката из вешенки обыкновенной. Применяемые дозы облучения составили 1, 3, 6 и 9 кГр. Полученные данные показали, что при облучении полуфабриката от 1 до 6 кГр в нем высвобождается большее количество белка по сравнению с необлученными образцами, а также увеличивается содержание ряда незаменимых аминокислот. Следовательно, рассматриваемый метод позволяет увеличить биологическую активность нутриентов. Ионизирующее излучение замедляет рост микроорганизмов и тем самым оказывает стерилизующий эффект, который был достигнут при дозе облучения образцов 6 кГр: патогенные микроорганизмы не были обнаружены, их рост не наблюдался в течение 21 дня. Таким образом, электронное ионизирующее излучение как часть технологического процесса переработки грибов позволяет увеличить биологическую ценность белка, препятствует размножению микроорганизмов, продлевая тем самым срок хранения грибной продукции.</p></abstract><trans-abstract xml:lang="en"><p>The question of the biological value of mushroom protein is not limited to the study of its composition. In addition, the associated problem of its digestibility and assimilability is widely discussed in the scientific community, including in the scientific publications discussed in this article. Mycoprotein derived from proteinrich unicellular fungi biomass is commonly used in contemporary food-production processes. In Russia, however, an alternative source of protein consists in the higher fungi including the common oyster mushroom (Pleurotus ostreatus). It is known that fungal proteins having a rich amino acid composition are absorbed at the level of plant proteins, since they are “packed” in the intercellular chitinous membrane. Various technological methods are used in the processing of raw fungi materials in order to destroy the membrane and release the protein macromolecule. These include traditional processing methods, such as grinding, thermal exposure, etc. In the present article, electromagnetic sterilisation of convenience foods is considered as a means of improving product safety. The authors studied the effect of ionising electron radiation on the physicochemical properties of mushroom products using the example of a heat-treated, vacuum-packaged, semi-finished oyster mushroom product. The applied radiation doses were 1, 3, 6 and 9 kGy. The obtained data showed that, when a semifinished product is irradiated with a dose of 1 to 6 kGy, a greater amount of protein is released compared to unirradiated samples; moreover, that the content of a number of essential amino acids also increases. Therefore, this method allows the biological activity of the nutrients to be increased. The sterilising effect of ionising radiation was achieved with a dose of 6 kGy due to slowing the growth of microorganisms: no pathogenic microorganisms were detected and no growth was observed over a period of 21 days. Thus, electronic ionising radiation as part of the technological process of processing mushrooms allows the biological value of the protein to be increased, preventing the growth of microorganisms and thereby extending the shelf life of mushroom products.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>электронная стерилизация</kwd><kwd>вешенка обыкновенная</kwd><kwd>грибной полуфабрикат</kwd><kwd>аминокислотный состав белка</kwd><kwd>ионизирующее излучение</kwd><kwd>сроки хранения</kwd></kwd-group><kwd-group xml:lang="en"><kwd>electromagnetic sterilisation</kwd><kwd>oyster mushroom</kwd><kwd>semi-finished mushroom</kwd><kwd>amino acid composition of protein</kwd><kwd>ionising radiation</kwd><kwd>shelf life</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Лисин П.А., Мусина О.Н., Кистер И.В., Чернопольская Н.Л. Методология оценки сбалансированности аминокислотного состава многокомпонентных пищевых продуктов // Вестник Омского государственного аграрного университета. 2013. N 3 (11). С. 53–58.</mixed-citation><mixed-citation xml:lang="en">Lisin P.A., Musina O.N., Kister I.V., Chernopolskaya N.L. Evaluation of sound amino acid composition of multifood. Vestnik Omskogo gosudarstvennogo agrarnogo universiteta. 2013, no. 3 (11), pp. 53–58. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Липатов Н.Н. Некоторые аспекты моделирования аминокислотной сбалансированности пищевых продуктов // Пищевая и перерабатывающая промышленность. 1986. N 4. С. 48–51.</mixed-citation><mixed-citation xml:lang="en">Lipatov N.N. Some aspects of amino acid balance modeling. Pishchevaya i pererabatyvayushchaya promyshlennost'. 1986, no. 4, pp. 48–51. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Липатов Н.Н., Сажинов Г.Ю., Башкиров О.Н. Формализованный анализ амино- и жирокислотной сбалансированности сырья, перспективного для проектирования продуктов детского питания с задаваемой пищевой адекватностью // Хранение и переработка сельхозсырья. 2001. N 8. С. 11–14.</mixed-citation><mixed-citation xml:lang="en">Lipatov N.N., Sazhinov G.Yu., Bashkirov O.N. Formalized analysis of amino and fatty acid balance of raw materials, promising for the design of baby food with specified nutritional adequacy. Khranenie i pererabotka sel'khozsyr'ya. 2001, no. 8, pp. 11–14. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Boutrif E. Recent developments in protein quality evaluation // Food, Nutrition and Agriculture. 1991. Issue 2/3. Available at: http://www.fao.org/3/U5900t/u5900t07.htm (accessed 28.06.2019)</mixed-citation><mixed-citation xml:lang="en">Boutrif E. Recent developments in protein quality evaluation. Food, Nutrition and Agriculture. 1991, issue 2/3. Available at: http://www.fao.org/3/U5900t/u5900t07.htm (accessed 28.06.2019)</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Schaafsma G. The Protein Digestibility-Corrected Amino Acid Score (PDCAAS) – a concept for describing protein quality in foods and food ingredients: a critical review // Journal of AOAC International. 2005. Vol. 88. Issue 3. P. 988–994.</mixed-citation><mixed-citation xml:lang="en">Schaafsma G. The Protein Digestibility-Corrected Amino Acid Score (PDCAAS) – a concept for describing protein quality in foods and food ingredients: a critical review. Journal of AOAC International. 2005, vol. 88, issue 3, pp. 988–994.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Dietary protein quality evaluation in human nutrition. Report of an FAO Expert Consultation, 31 March – 2 April 2011, Auckland, New Zealand. FAO Food and Nutrition Paper. Vol. 92. Rome. Food Agriculture Organization of the United Nations. Rome, 2013. 66 p. Available at: http://www.fao.org/ag/humannutrition/35978-02317b979a686a57aa4593304ffc17f06.pdf (accessed 28.06.2019)</mixed-citation><mixed-citation xml:lang="en">Dietary protein quality evaluation in human nutrition. Report of an FAO Expert Consultation, 31 March – 2 April 2011, Auckland, New Zealand. FAO Food and Nutrition Paper, vol. 92. Rome. Food Agriculture Organization of the United Nations. Rome, 2013, 66 p. Available at: http://www.fao.org/ag/humannutrition/35978-02317b979a686a57aa4593304ffc17f06.pdf (accessed 28.06.2019)</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Sarwar G. The protein digestibility-corrected amino acid score method overestimates quality of proteins containing antinutritional factors and of poorly digestible –proteins supplemented with limiting amino acids in rats // The Journal of Nutrition. 1997. Vol. 127. Issue 5. P. 758–764. DOI: 10.1093/jn/127.5.758</mixed-citation><mixed-citation xml:lang="en">Sarwar G. The protein digestibility-corrected amino acid score method overestimates quality of proteins containing antinutritional factors and of poorly digestible –proteins supplemented with limiting amino acids in rats. The Journal of Nutrition. 1997, vol. 127, issue 5, pp. 758–764. DOI: 10.1093/jn/127.5.758</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Denny A., Aisbitt B., Lunn J. Mycoprotein and health // Nutrition Bulletin. 2008. Vol. 33. Issue 4. P. 298–310. DOI: 10.1111/j.1467-3010.2008.00730.x</mixed-citation><mixed-citation xml:lang="en">Denny A., Aisbitt B., Lunn J. Mycoprotein and health. Nutrition Bulletin. 2008, vol. 33, issue 4, pp. 298–310. DOI: 10.1111/j.1467-3010.2008.00730.x</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Edwards D.G., Cummings J.H. The protein quality of mycorprotein // Proceedings of the Nutrition Society. 2010. Vol. 69. Issue OCE4. P. E331. DOI: 10.1017/S0029665110001400</mixed-citation><mixed-citation xml:lang="en">Edwards D.G., Cummings J.H. The protein quality of mycorprotein. Proceedings of the Nutrition Society. 2010, vol. 69, issue OCE4, pp. E331. DOI: 10.1017/S0029665110001400</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Wiebe M. Myco-protein from Fusarium venenatum: a well-established product for human consumption // Applied Microbiology and Biotechnology. 2002. Vol. 58. Issue 4. P. 421–427. DOI: 10.1007/s00253-002-0931-x</mixed-citation><mixed-citation xml:lang="en">Wiebe M. Myco-protein from Fusarium venenatum: a well-established product for human consumption. Applied Microbiology and Biotechnology. 2002, vol. 58, issue 4, pp. 421–427. DOI: 10.1007/s 00253-002-0931-x</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Wiilamson D.A., Geiselman P.J., Lovejoy J., Greenway F., Volaufova J., Martin C.K., Arnett C., Ortego L. Effects of consuming mycoprotein, tofu or chicken upon subsequent eating behaviour, hunger and safety // Appetite. 2006. Vol. 46. Issue 1. P. 41–48. DOI: 10.1016/j.appet.2005.10.007</mixed-citation><mixed-citation xml:lang="en">Wiilamson D.A., Geiselman P.J., Lovejoy J., Greenway F., Volaufova J., Martin C.K., Arnett C., Ortego L. Effects of consuming mycoprotein, tofu or chicken upon subsequent eating behaviour, hunger and safety. Appetite. 2006, vol. 46, issue 1, pp. 41–48. DOI: 10.1016/j.appet.2005.10.007</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Радиационные технологии в сельском хозяйстве и пищевой промышленности / под общ. ред. Г.В. Козьмина, С.А. Гераськина и Н.И. Санжаровой. Обнинск: Ид-во ВНИИРАЭ, 2015. 400 с.</mixed-citation><mixed-citation xml:lang="en">Radiatsionnye tekhnologii v sel'skom khozyaistve i pishchevoi promyshlennosti [Radiation technologies in agriculture and food industry]. Ed. by G.V. Koz’min, S.A. Geras’kin, N.I. Sanzharova. Obninsk: Russian Institute of Radiology and Agroecology Publ., 2015, 400 p.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Fernandes A., Barreira J.C.M., Antonio A.L., Martins A., Ferreira I.C.F.R. Triacylglycerols profiling as a chemical tool to identify mushrooms submitted to gamma or electron beam irradiation // Food Chemistry. 2014. Vol. 159. P. 399–406. DOI: 10.1016/j.foodchem.2014.03.035</mixed-citation><mixed-citation xml:lang="en">Fernandes A., Barreira J.C.M., Antonio A.L., Martins A., Ferreira I.C.F.R. Triacylglycerols profiling as a chemical tool to identify mushrooms submitted to gamma or electron beam irradiation. Food Chemistry. 2014, vol. 159, pp. 399–406. DOI: 10.1016/j.foodchem.2014.03.035</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Mami Y., Peyvast G., Ziaie F., Ghasemnezad M., Salmanpour V. Improvement of shelf life and postharvest quality of white mushroom by electron beam irradiation // Journal of Food Processing and Preservation. 2014. Vol. 38. Issue 4. P. 1673–1681. DOI: 10.1111/jfpp.12129</mixed-citation><mixed-citation xml:lang="en">Mami Y., Peyvast G., Ziaie F., Ghasemnezad M., Salmanpour V. Improvement of shelf life and postharvest quality of white mushroom by electron beam irradiation. Journal of Food Processing and Preservation. 2014, vol. 38, issue 4, pp. 1673–1681. DOI: 10.1111/jfpp.12129</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang K.X., Pu Y.Y., Sun D.W. Recent advances in quality preservation of postharvest mushrooms (Agaricus bisporus): A review // Trends in Food Science &amp; Technology. 2018. Vol. 78. P. 72–82. DOI: 10.1016/j.tifs.2018.05.012</mixed-citation><mixed-citation xml:lang="en">Zhang K.X., Pu Y.Y., Sun D.W. Recent advances in quality preservation of postharvest mushrooms (Agaricus bisporus): A review. Trends in Food Science &amp; Technology. 2018, vol. 78, pp. 72–82. DOI: 10.1016/j.tifs.2018.05.012</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Abdeldaiem M.H.M., Ali H.G.M., Hassanien M.F.R. Extending the shelf life of refrigerated beef balls using methanol extracts of gamma-irradiated mushrooms // Fleischwirtschaft. 2016. Vol. 96. Issue 11. P. 107–114.</mixed-citation><mixed-citation xml:lang="en">Abdeldaiem M.H.M., Ali H.G.M., Hassanien M.F.R. Extending the shelf life of refrigerated beef balls using methanol extracts of gamma-irradiated mushrooms. Fleischwirtschaft. 2016, vol. 96, issue 11, pp. 107–114.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Cardoso R.V.C., Fernandes Â., Barreira J.C.M., Verde S.C., Antonio A.L., Gonzaléz-Paramás A.M., Barros L., Ferreira I.C.F.R. Effectiveness of gamma and electron beam irradiation as preserving technologies of fresh Agaricus bisporus Portobello: A comparative study // Food Chemistry. 2019. Vol. 278. P. 760–766. DOI: 10.1016/j.foodchem.2018.11.116</mixed-citation><mixed-citation xml:lang="en">Cardoso R.V.C., Fernandes Â., Barreira J.C.M., Verde S.C., Antonio A.L., Gonzaléz-Paramás A.M., Barros L., Ferreira I.C.F.R. Effectiveness of gamma and electron beam irradiation as preserving technologies of fresh Agaricus bisporus Portobello: A comparative study. Food Chemistry. 2019, vol. 278, pp. 760–766. DOI: 10.1016/j.foodchem.2018.11.116</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Kortei N.K., Odamtten G.T., Obodai M., WiafeKwangyan M., Addo E.A. Influence of low dose of gamma radiation and storage on some vitamins and mineral elements of dried oyster mushrooms (Pleurotus ostreatus) // Food Science &amp; Nutrition. 2017. Vol. 5. Issue 3. P. 570–578. DOI: 10.1002/fsn3.432</mixed-citation><mixed-citation xml:lang="en">Kortei N.K., Odamtten G.T., Obodai M., WiafeKwangyan M., Addo E.A. Influence of low dose of gamma radiation and storage on some vitamins and mineral elements of dried oyster mushrooms (Pleurotus ostreatus). Food Science &amp; Nutrition. 2017, vol. 5, issue 3, pp. 570–578. DOI: 10.1002/fsn3.432</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Tejedor-Calvo E., Morales D., Marco P., Venturini M.E., Blanco D., Soler-Rivas C. Effects of combining electron-beam or gamma irradiation treatments with further storage under modified atmospheres on the bioactive compounds of Tuber melanosporum truffles // Postharvest Biology and Technology. 2019. Vol. 155.P.149–155.DOI:10.1016/j.postharvbio.2019.05.022</mixed-citation><mixed-citation xml:lang="en">Tejedor-Calvo E., Morales D., Marco P., Venturini M.E., Blanco D., Soler-Rivas C. Effects of combining electron-beam or gamma irradiation treatments with further storage under modified atmospheres on the bioactive compounds of Tuber melanosporum truffles. Postharvest Biology and Technology. 2019, vol. 155, pp. 149–155. DOI: 10.1016/j.postharvbio.2019.05.022</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">ТепляковаТ.В., Косогова Т.А., Ананько Г.Г., Бардашева А.В., Ильичева Т.Н. Противовирусная активность базидиальных грибов. Обзор литературы // Проблемы медицинской микологии. 2014. Т. 16. N 2. С. 15–25.</mixed-citation><mixed-citation xml:lang="en">Teplyakova T.V., Kosogova T.A., Anan'ko G.G., Bardasheva A.V., Il'icheva T.N. Antiviral activity of basidiomycetes. Review of literature. Problemy meditsinskoi mikologii.2014, vol. 16, no. 2, pp. 15–25.(In Russian)</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
