vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2017-7-2-43-53vuzbiochemi-31Research ArticleФИЗИКО-ХИМИЧЕСКАЯ И ОБЩАЯ БИОЛОГИЯPHYSICOCHEMICAL AND GENERAL BIOLOGYИДЕНТИФИКАЦИЯ И ХАРАКТЕРИСТИКА ОСНОВНЫХ ВТОРИЧНЫХ МЕТАБОЛИТОВ ГРИБОВ FUSARIUMTRICINCTUMIDENTIFICATION AND CHARACTERIZATION OF MAJOR SECONDARY METABOLITES FROM THE FUNGUS FUSARIUM TRICINCTUMМохамедХ. ..MohamedH. ..noemail@neicon.ruПетерсонА. М.PetersonA. M.noemail@neicon.ruЕбрагемВ. ..EbrahimcW. ..noemail@neicon.ruПрокшП. ..ProkschP. ..noemail@neicon.ruСаратовский государственный национальный исследовательский университетРоссияDepartment of Microbiology and Plant Physiology, Faculty of Biology, Saratov State UniversityRussian FederationУниверситет Генриха ГейнеРоссияHeinrich-Heine UniversityRussian Federation201723092019724453Copyright © Мохамед Х..., Петерсон А.М., Ебрагем В..., Прокш П..., 20192019Мохамед Х..., Петерсон А.М., Ебрагем В..., Прокш П...Mohamed H..., Peterson A.M., Ebrahimc W..., Proksch P...Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/31

Грибы рода Fusarium, паразитирующие на растениях, известны как продуценты разнообразных метаболитов с широкой биологической активностью. Метаболиты F. tricinctum ранее изучались на штаммах, изолированных преимущественно с пшеницы и кукурузы. Проведено исследование вторичных метаболитов F. tricinctum, изолированного из внутренних тканей побегов яблонь. Видовая принадлежность штамма подтверждена анализом последовательностей генов 18S рРНК. С помощью методов высокоэффективной жидкостной хроматографии (ВЭЖХ) обнаружено шесть вторичных метаболитов: энниатины (A, B, G, H), гибепирон B и ауреонитол. Идентификация данных соединений была проведена при помощи метода жидкостной хроматографии с масс-спектрометрическим детектированием (LC-MS). Выявлено, что неочищенный грибковый экстракт проявляет слабую антибактериальную активность в отношении Mycobacterium tuberculosis и Staphylococcus aureus.

Enniatins (ENs) are secondary fungal metabolites with hexadepsipeptidic chemical structure and they possess a number of potent biological activities and they are known to act as antifungal, anti yeast and antibacterial and to possess anti insecticidal and phytotoxic properties. ENs are produced by several Fusarium strains including Fusarium tricinctum, F. proliferatum and F. subglutinans. The endophyte F. tricinctum (F106) was isolated from the apple shoots in Volga region, Saratov, Russia. Molecular characterization performed by internal transcribed spacer (ITS1,4), rRNA gene sequence analysis and it was confirmed as F. tricinctum, accession number (KR184140). High-performance liquid chromatography (HPLC), investigation of the Ethyl acetate (EtOAc), crude extract of F. tricinctum which was grown on solid rice medium yielded six secondary metabolites, including Enniatins (A, B, G, H), in addition to other metabolites Gibepyrone B and Aureonitol. Fungal crude extract were found to potentiate activity against Mycobacterium tuberculosis and Staphyllococcus aureus. All these structures were determined on the basis of liquid chromatography-mass spectrometry (LC-MS).

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The authors declare that there are no conflicts of interest present.