vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2017-7-2-72-79vuzbiochemi-34Research ArticleФИЗИКО ХИМИЧЕСКАЯ И ОБЩАЯ БИОЛОГИЯPHYSICO-CHEMICAL AND GENERAL BIOLOGYСТРУКТУРА И ФИЗИКО-ХИМИЧЕСКИЕ СВОЙСТВА ГЛИКОЛИПИДНЫХ БИОСУРФАКТАНТОВ, ПРОДУЦИРУЕМЫХ БАКТЕРИЯМИ-НЕФТЕДЕСТРУКТОРАМИ RHODOCOCCUSSP. X5STRUCTURE AND PHYSICOCHEMICAL PROPERTIES OF GLYCOLIPID BIOSURFACTANT, PRODUCED BY OIL-DEGRADING BACTERIA RHODOCOCCUSSP. X5ЛыонгТ. М.Postgraduate StudentT. M.luongmo@mail.ruНечаеваИ. А.NechaevaI. A.nechaeva1902@gmail.comПетриковК. В.PetrikovK. V.bioscience.kp@gmail.comФилоновА. Е.FilonovA. E.filonov.andrey@rambler.ruПонамореваО. Н.PonamorevaO. N.olgaponamoreva@mail.ruТульский государственный университетРоссияTula State UniversityRussian FederationИнститут физиологии и биохимии микроорганизмов им. Г.К. Скрябина РАНРоссияG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of SciencesRussian Federation201723092019727279Copyright © Лыонг Т.М., Нечаева И.А., Петриков К.В., Филонов А.Е., Понаморева О.Н., 20192019Лыонг Т.М., Нечаева И.А., Петриков К.В., Филонов А.Е., Понаморева О.Н.Postgraduate Student T.M., Nechaeva I.A., Petrikov K.V., Filonov A.E., Ponamoreva O.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/34

Показано, что основными биосурфактантами, продуцируемыми Rhodococcus sp. X5 при росте на н-гексадекане, являются сукциноилтетраэфирами трегалозы. Структурная характеристика трегалолипидов была проведена методами тандемной масс-спетримерии и ГС-МС. Сукциноилтрегалолипиды, образуемые Rhodococcus sp. X5, обладают высокой поверхностной (27 мН/м) и эмульгирующейся активностью (E24= 55%). Биосурфактанты охарактеризованы по следующим физико-химичестким показателям - минимальное значение поверхностного натяжения (27 мН/м), критическая концентрация мицеллообразования (4,1.10-5 моль/л),гидрофильно-липофильный баланс (7-8), максимальное количесство молекул, адсорбируемых на единице площади раздела фаз (1,27.10-5 моль/м2), минимальная площадь, занимаемой одной молекулой биосурфактанта (13 нм2) и свободная энергия Гиббса (-35 кДжоль/моль). Установлено, что изучаемые трегалолипиды способны увеличивать смачиваемую водой поверхность гидрофобных веществ и образовывать эмульсии в виде «масло в воде».

N this study it was reported, that significant biosurfactants produced by Rhodococcus sp. X5 grown on n-hexadecane, are succinoyl trehalose tetraesters. The structural characterization of trehalolipids was determined based on MS/MS and GC/MS analysis. Succinoyl trehalolipids from Rhodococcus sp. X5 exhibit high surface activity (27 mN/m) and emulsifying activity (E24 - 55%). Biosurfactants were characterized by the next physico-chemical indications - minimum surface tension (27 mN/m), critical micelle concentration (4,1·10-5 mol/l),hydrophilic - lipophilic balance (7-8), the maximum number of molecule adsorbed per unit area (1,27·10-5mol/m2), the minimum area per molecule biosurfactant (13 nm2), Gibbs free energy of adsorption (-35 kJ/mol). The results demonstrate that studied trehalolids are capable to increase wetting the surface of hydophobic compounds by water and to form type of emulsion “oil/water”.

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The authors declare that there are no conflicts of interest present.