vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2020-10-3-459-469vuzbiochemi-419Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВлияние условий культивирования на продуцирование ксиланазы и рост бактерий Paenibacillus mucilaginosusImpact of cultivation conditions on xylanase production and growth in Paenibacillus mucilaginosusХаТ. З.HaD. T.

Ха Тхи Зунг, аспирант кафедры пищевой биотехнологии

420015, г. Казань, ул. Толстова, 8

Dung T. Ha, Postgraduate Student, Department of Food Biotechnology

8, Tolstov St., Kazan, 420015

coldwind.91@mail.ruКанарскийА. В.KanarskiyA. V.

Канарский Альберт Владимирович, д.т.н., профессор кафедры пищевой биотехнологии

420015, г. Казань, ул. Толстова, 8

Albert V. Kanarsky, Dr. Sci. (Engineering), Professor, Department of Food Biotechnology

8, Tolstov St., Kazan, 420015

alb46@mail.ruКанарскаяЗ. А.KanarskayaZ. A.

Канарская Зося Альбертовна, к.т.н., доцент кафедры пищевой биотехнологии

420015, г. Казань, ул. Толстова, 8

Zosia A. Kanarskaya, Cand. Sci. (Engineering), Associate Professor Department of Food Biotechnology

8, Tolstov St., Kazan, 420015

zosya_kanarskaya@mail.ruЩербаковА. В.ScherbakovA. V.

Щербаков Андрей Владимирович, к.б.н., научный сотрудник лаборатории технологии микробных препаратов

196608, С.-Петербург, шоссе Подбельского, 3

Andrei V. Shcherbakov, Cand. Sci. (Biology), Researcher, Laboratory of Microbial Technology

3, Podbelsky Highway, St. Petersburg, 196608

microbe-club@inbox.ruЩербаковаЕ. Н.ScherbakovaE. N.

Щербакова Елена Николаевна, к.с-х.н., младший научный сотрудник лаборатории технологии микробных препаратов

196608, С.-Петербург, шоссе Подбельского, 3

Elena N. Shcherbakova, Cand. Sci. (Agriculture), Researcher, Laboratory of Microbial Technology

3, Podbelsky Highway, St. Petersburg, 196608

alonagonchar@mail.ruПрановичА. В.PranovichA. V.

Пранович Андрей Викторович, к.х.н., доцент кафедры химии древесины и бумаги

Туомиокирконтори 3, Турку/Або, FI-20500, Финляндия

Andrey V. Pranovich, Cand. Sci. (Chemistry), Associate Professor, Department of Wood Chemistry, Laboratory of Wood and Paper Chemistry

3, Tuomiokirkontori, Turku/Abo, FI-20500, Finland

apranovi@abo.fiКазанский национальный исследовательский технологический университет (КНИТУ)РоссияKazan National Research Technological UniversityRussian FederationВНИИ сельскохозяйственной микробиологии (ВНИИСХМ)РоссияAll-Russia Research Institute for Agricultural MicrobiologyRussian FederationАбо АкадемияФинляндияAbo AcademyFinland202008102020103459469Copyright © Ха Т.З., Канарский А.В., Канарская З.А., Щербаков А.В., Щербакова Е.Н., Пранович А.В., 20202020Ха Т.З., Канарский А.В., Канарская З.А., Щербаков А.В., Щербакова Е.Н., Пранович А.В.Ha D.T., Kanarskiy A.V., Kanarskaya Z.A., Scherbakov A.V., Scherbakova E.N., Pranovich A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/419

Ксиланаза – фермент, гидролизующий β-1,4-связи в ксиланах. Этот фермент используется для получения из отходов агропромышленного комплекса гидролизата ксилоолигосахаридов с целью улучшения энергетической ценности и повышения усвояемости кормов для животных, обработки пищевых продуктов, утилизации и ускорения разложения поживных остатков в почве, а также в технологии отбелки целлюлозы и других областях. В настоящее время представляет интерес использование возобновляемых ресурсов, в частности, отходов сельскохозяйственного производства, в качестве субстрата вместо дорогого очищенного ксилана для скрининга продуцентов и выделения ксиланаз. Целью настоящей работы являлось определение влияния условий культивирования бактерий Paenibacillus mucilaginosus на продуцирование ксиланаз. В качестве источника углерода использовали ферментолизат рисовой шелухи, ксилан, выделенный из березы и бука. Изучено влияние температуры, рН среды, факторов корректировки рН среды, продолжительности инкубации инокулята, источ-ников углерода и азота, и также их концентраций на биосинтез ксиланаз и рост штамма 560 P. mucilaginosus. Установлено, что для биосинтеза кисланазы культивирование штамма 560 P. mucilaginosus перспективно и экономически целесообразно проводить на питательной среде, приготовленной на основе ферментолизата рисовой шелухи. Присутствующие в составе ферментолизата рисовой шелухи индукторы улучшают биосинтез ксиланаз. Показано положительное влияние ионов кальция на биосинтез ксиланаз у рассматриваемого штамма. Рекомендуемые условия культивирования: концентрация источника углерода в питательной среде по общему количеству РВ – 0,5%; в качестве источника азота целесообразно использовать 0,2% карбамид; при корректировке рН среды до 6,0±0,2 необходим гидроксид кальция; температура культивирования бактерий – 30±1 °С. В указанных условиях культивирования P. mucilaginosus не требуется предварительного приготовления посевного материала, а максимальная активность синтезируемой ксиланазы в стационарной фазе роста бактерий достигает значения 20 ед./мл.

Xylanase is an enzyme that hydrolyses β-1,4 bonds in plant xylan. This enzyme is applied in the bioconversion of agro-industrial waste for xylooligosaccharide hydrolysate production to improve digestibility and nutrition value of animal feed, food processing, the utilisation and faster decomposition of crop debris in soil, as well as in cellulose bleaching and other industries. The current trend focuses on using renewable resources, such as agricultural waste, as substitutes for expensive purified xylan in producer screening and xylanase synthesis. This work aimed to determine the impact of Paenibacillus mucilaginosus cultivation conditions on the xylanase production yield. Rice bran ferment lysate along with birch and beech timber xylans were used as a carbon source. Temperature, medium pH, pH correction factors, inoculant incubation time, carbon and nitrogen sources and concentrations were the studied criteria of xylanase biosynthesis and growth in bacteria P. ucilaginosus strain 560. We show that the xylanase biosynthesis and cultivation in P. mucilaginosus strain 560 are more practical and cost-effective with the use of a rice bran ferment lysate-based nutrient medium. Inductors contained in the rice bran ferment lysate improve the xylanase biosynthesis. Calcium ions also facilitate biosynthesis in the studied strain. Cultivation recommendations are: carbon source concentration in medium 0.5% of total reducing substances content; 0.2% carbamide as optimal nitrogen source; calcium hydroxide as an agent for medium pH correction to 6.0±0.2; cultivation temperature 30±1 °С. Under the specified conditions, cultivation of P. mucilaginosus does not require inoculate preprocessing, and a maximal xylanase activity in stationary culture reaches 20 U/mL.

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The authors declare that there are no conflicts of interest present.