vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2020-10-4-613-626vuzbiochemi-476Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYИсследование потенциальных антиканцерогенных и антиоксидантных эффектов экстрактов из растительного сырьяStudy of potential anti-carcinogenic and antioxidant effects of plant extractsЕремееваН. Б.EremeevaN. B.

Еремеева Наталья Борисовна - кандидат технических наук, доцент кафедры технологии и организации общественного питания.

443100, Самара, ул. Молодогвардейская, 244

Natalya B. Eremeeva - Cand. Sci. (Engineering), Associate Professor, Department of Technology and Organization of Catering.

244, Molodogvardeiskaya St., Samara, 443100

eremeeva.nb.samgtu@gmail.comМакароваН. В.MakarovaN. V.

Макарова Надежда Викторовна - доктор химических наук, профессор, заведующая кафедрой технологии и организации общественного питания.

443100,  Самара, ул. Молодогвардейская, 244

Nadezhda V. Makarova - Dr. Sci. (Chemistry), Professor, Head of Department of Technology and Organization of Catering.

244, Molodogvardeiskaya St., Samara, 443100

makarovanv1969@yandex.ruИгнатоваД. Ф.IgnatovaD. F.

Игнатова Динара Фанисовна - кандидат технических наук, доцент кафедры технологии и организации общественного питания, Самарский государственный технический университет,

443100, Самара, ул. Молодогвардейская, 244

Dinara F. Ignatova - Cand. Sci. (Engineering), Associate Professor, Department of Technology and Organization of Catering.

244, Molodogvardeiskaya St., Samara, 443100

dinara-bakieva@mail.ruБахаревВ. В.BakharevV. V.

Бахарев Владимир Валентинович - доктор химических наук, профессор, декан факультета пищевых производств.

443100, Самара, ул. Молодогвардейская, 244

Vladimir V. Bakharev - Dr. Sci. (Chemistry), Professor, Dean of Faculty of Food Production.

244, Molodogvardeiskaya St., Samara, 443100

fpp@samgtu.ruСамарский государственный технический университетРоссияSamara State Technical UniversityRussian Federation202007012021104613626Copyright © Еремеева Н.Б., Макарова Н.В., Игнатова Д.Ф., Бахарев В.В., 20212021Еремеева Н.Б., Макарова Н.В., Игнатова Д.Ф., Бахарев В.В.Eremeeva N.B., Makarova N.V., Ignatova D.F., Bakharev V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/476

Одним из основных этиологических факторов возникновения некоторых видов злокачественных опухолей является наличие в организме хронических воспалительных процессов. В ряде медицинских исследований для противовоспалительных лекарственных средств показан антиканцерогенный эффект относительно рака толстого кишечника. Кроме того, имеются данные о взаимосвязи антиканцерогенной и антиоксидантной активностей некоторых биологически активных веществ, присутствующих в лекарственных растениях. В настоящей работе исследованы потенциальные антиканцерогенные эффекты экстрактов, полученных из растительного сырья: брусники (Vaccmium vftis-idaea), малины (Rubus idaeus), черноплодной рябины (Aronia melanocarpa), семян винограда (Vitis L.), выжимок винограда^№ L.), зеленого краснодарского чая (Camellia sinensis), женьшеня (Panax L.), иван-чая (Chamaenerion angustifolium) и кофе (Coffea arabica). Оценено влияние данных экстрактов на рост и жизнеспособность клеток рака толстого кишечника линии НСТ-116 in vitro с помощью МТТ-теста, а также влияние нетоксических доз экстрактов на базальный и индуцированный уровень экспрессии провоспалительных генов: циклооксигеназы (COX2); индуцируемой NO-синтазы (iNOS); интерлейкина 8 (IL8), методом количественной полимеразной цепной реакции. Определены рабочие концентрации исследуемых экстрактов для клеток НСТ-116. Продемонстрировано, что все исследуемые экстракты способны снижать экспрессию провоспалительных генов COX-2, iNOS и IL-8. Наиболее выраженным суммарным ингибирующим действием на экспрессию данных генов обладают экстракты черноплодной рябины и иван-чая. Данные экстракты рекомендуется использовать в дальнейших исследованиях влияния на индуцированную экспрессию генов COX-2, iNOS и IL-8, а также в исследованиях антиканцерогенной активности in vivo. Антиокисдантная активность определена по методу DPPH и FRAP. Наибольшие значения по данным методикам показали экстракты черноплодной рябины, малины и иван-чая. Выявлена зависимость антиканцерогенной и антиоксидантной активностей.

One of the main etiological factors in the development of some malignant tumours is the presence of chronic inflammatory processes. A number of biomedical studies have confirmed the anticarcinogenic effect of anti-inflammatory drugs with respect to colon cancers. In addition, some biologically active substances present in medicinal plants have been found to exhibit both anti-carcinogenic and antioxidant activity. In this paper, we investigate potential anticarcinogenic effects of extracts obtained from plant raw materials, including lingonberry (Vaccinium vttis-idaea), raspberry (Rubus idaeus), black chokeberry (Aronia melanocarpa), grape seeds (Vitis L.), grape pomace (Vitis L.), Krasnodar green tea (Camellia sinensis), ginseng (Panax L.), fireweed (Chamaenerion angustifolium) and coffee (Coffea arabica). The effect of these extracts on the growth and viability of colon HCT-116 cancer cells was assessed in vitro using the method of MTT assay. In addition, the effect of non-toxic doses of the extracts under study on the basal and induced levels of pro-inflammatory gene expression - cyclooxygenase (COX2), inducible NO synthase (iNOS) and interleukin 8 (IL8) - was assessed by quantitative PCR. The working concentrations of the extracts under study for HCT-116 cells were determined. It is demonstrated that all investigated extracts are capable of suppressing the expression of COX-2, iNOS and IL-8. Black chokeberry and fireweed extracts exhibit the most pronounced total inhibitory effect on the expression of these inflammatory genes. Future research should investigate effects of black chokeberry and fireweed extracts on the induced expression of COX-2, iNOS and IL-8 and their anti-carcinogenic activity in vivo. The antioxidant activity determined using DPPH and FRAP was the highest for black chokeberry, raspberry and fireweed. A relationship between the anticar-cinogenic and antioxidant effects of the extracts under study was revealed.

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The authors declare that there are no conflicts of interest present.