vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2020-10-4-639-646vuzbiochemi-478Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВыявление соединения, антигеноподобного трийодтиронину, в клетках высших растенийIdentification of a compound similar to triiodothyronine in the cells of higher plantsГариповаМ. И.GaripovaM. I.

Гарипова Маргарита Ивановна - доктор биологических наук, доцент, профессор кафедры биохимии и биотехнологии.

450076, Уфа, ул. Заки Валиди, 32

Margarita I. Garipova - Dr. Sci. (Biology), Associate Professor, Professor, Biochemistry and Biotechnology Department.

32, Zaki Validy St., Ufa, 450076

margaritag@list.ruФедяевВ. В.FedyaevV. V.

Федяев Вадим Валентинович - кандидат биологических наук, доцент кафедры биохимии и биотехнологии.

450076, Уфа, ул. Заки Валиди, 32

Vadim V. Fedyaev - Cand. Sci. (Biology), Associate Professor, Biochemistry and Biotechnology Department.

32, Zaki Validy St., Ufa, 450076

vadim.fedyaev@gmail.comФархутдиновР. Г.FarkhutdinovR. G.

Фархутдинов Рашит Габдулхаевич - доктор биологических наук, доцент, профессор кафедры биохимии и биотехнологии.

450076, Уфа, ул. Заки Валиди, 32

Rashit G. Farkhutdinov - Dr. Sci. (Biology), Associate Professor, Professor, Biochemistry and Biotechnology Department.

32,          Zaki Validy St., Ufa, 450076

frg2@mail.ruСотниковаЮ. М.SotnikovaJ. M.

Сотникова Юлия Михайловна - старший преподаватель кафедры биохимии и биотехнологии.

450076, Уфа, ул. Заки Валиди, 32

Julia M. Sotnikova - Senior Lecturer, Biochemistry and Biotechnology Department.

32, Zaki Validy St., Ufa, 450076

sotnikova-bashedu@mail.ruБашкирский государственный университетРоссияBashkir State UniversityRussian Federation202007012021104639646Copyright © Гарипова М.И., Федяев В.В., Фархутдинов Р.Г., Сотникова Ю.М., 20212021Гарипова М.И., Федяев В.В., Фархутдинов Р.Г., Сотникова Ю.М.Garipova M.I., Fedyaev V.V., Farkhutdinov R.G., Sotnikova J.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/478

Общеизвестно метаболическое значение йодтиронинов в организме человека и животных. Для растений подобные соединения не описаны, однако, известны эффекты лекарственных растений на функцию щитовидной железы, что предполагает возможность присутствия соединений, аналогичных тиреоидным гормонам в клетках растений. Цель работы - выявление в клетках высших растений биологически наиболее активного представителя йодтиронинов - 3,5,3’-трийодтиронина. Методом иммуноферментного анализа показано, что в тканях корня и стебля проростков фасоли присутствует соединение, взаимодействующее с поликлональной сывороткой к 3,5,3’-трийодтиронину. Установлено, что в клетке выявленное соединение распределено неравномерно: в ядрах клеток его концентрация в 7-10 раз выше, чем в цитоплазме. Наряду с растениями фасоли исследованы 8 случайно выбранных растений, принадлежащих как к двудольным, так и однодольным растениям. Во всех исследованных растениях методом иммуноферментного анализа определено присутствие аналога 3,5,3’-трийодтиронина. Добавление экзогенного йодтиронина - тироксина (3,5,3’,5’-тетрайодтирони-на) при предпосевной обработке семян пшеницы достоверно повысило их всхожесть и привело к значимому увеличению соотношения массы побега к массе корня пятисуточных растений пшеницы при времени замачивания 6 и 12 ч. Вследствие обработки семян пшеницы тироксином в ядрах клеток стебля и корня пятидневных растений пшеницы произошло достоверное снижение концентрации выявленного соединения. Накопление в ядрах клеток и выявленные физиологические эффекты экзогенного 3,5,3’,5’-тетрайодтиронина позволяют предположить, что в клетках высших растений данное соединение принимает участие в регуляции активности генов подобно тому, как это имеет место в клетках животных.

Iodothyronines play an important role in human and animal metabolism; however, their action in plant species is yet to be described. The known beneficial effects of medicinal plants on the function of the thyroid gland suggest the presence of compounds similar to thyroid hormones in plant cells. In this work, we aim to reveal the presence of 3,5,3'-triiodothyronine, the most active representative of iodothyronines, in the cells of higher plants. Using the method of immunoenzyme analysis, it was determined that the roots and stems of bean seedlings contain a compound interacting with a polyclonal serum to 3,5,3'-triiodothyronine. The identified compound is distributed unevenly within the cell: its concentration in the cell nuclei is 7-10 times higher than that in the cytoplasm. Along with bean plants, 8 randomly selected plants belonging to both dicotyledonous and monocotyledonous families were studied. In all the studied species, the presence of an analogue of 3,5,3'-triiodothyronine was determined using the method of immunoenzyme analysis. The use of exogenous iodothyronine - thyroxine (3,5,3',5'-tetraiodothyronine) - for the pre-sowing treatment of wheat seeds reliably improved their germination and led to a significant increase in the root-shoot mass ratio of five-day-old wheat seedlings under the soaking time of 6 and 12 hours. The treatment of wheat seeds with thyroxine led to a significant decrease in the concentration of the identified compound in the stem and root cell nuclei of five-day-old wheat seedlings. The revealed physiological and accumulation effects of exogeus 3,5,3',5' tetraiodothyronine suggest its involvement in gene regulation in the cells of higher plants, similarly to that in animal cells.

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The authors declare that there are no conflicts of interest present.