vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2020-10-4-647-656vuzbiochemi-479Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYОсобенности влияния штамма Bacillus thuringiensis 0271 на отдельные показатели неспецифической резистентности Origanum vulgare L. к стрессовым условиямEffects of Bacillus thuringiensis 0271 on individual indicators of nonspecific resistance of Origanum vulgare L. to stressful conditionsКрыжкоА. В.KryzhkoA. V.

Крыжко Анастасия Владимировна - кандидат сельскохозяйственных наук, ведущий научный сотрудник лаборатории молекулярной генетики, протеомики и биоинформатики в сельском хозяйстве.

295453, Симферополь, ул. Киевская, 150

Anastasiia V. Kryzhko - Cand. Sci. (Agriculture), Leading Researcher, Laboratory of Molecular Genetics, Proteomics and Bioinformatics in Agriculture.

solanum@ukr.netБуджуроваУ. М.BudzhurovaU. M.

Буджурова Урьяне Меметовна - лаборант лаборатории молекулярной и клеточной биологи.

295453, Симферополь, ул. Киевская, 150

Uryane M. Budzhurova - Laboratory Assistant, Laboratory of Molecular and Cellular Biology.

150, Kievskaya St., Simferopol, 295453

ubudzhurova@mail.ruНаучно-исследовательский институт сельского хозяйства КрымаРоссияResearch Institute of Agriculture of CrimeaRussian Federation202007012021104647656Copyright © Крыжко А.В., Буджурова У.М., 20212021Крыжко А.В., Буджурова У.М.Kryzhko A.V., Budzhurova U.M.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/479

Цель - изучить влияние штамма Bacillus thuriengiensis var. darmstadiensis 0271 на биохимические показатели, определяющие неспецифическую устойчивость Origanum vulgare L. к неблагоприятным условиям внешней среды. Материалом исследований служила жидкая споровая культура штамма B. thuringiensis 0271, сортообразцы душицы обыкновенной: 100.1 с содержанием 75,5% карвакрола, г-4 с содержанием 52,0% карвакрола, № 2 с содержанием 59,85% а-терпинеола, № 1 с преимущественным содержанием гермакрена D (21,5%) и в-кариофиллена (19,4%). Динамику сохранности спор штамма на поверхности листьев O. vulgare L. определяли по методике А.Г. Кольчевского, содержание пролина - по методике В.А. Храмова и Е.М. Агеевой. Содержание пигментов измеряли спектрофотометрически. Определение суммарного содержания фенольных соединений проводили по М.Н. Запрометову, аскорбиновой кислоты - по М.М. Окунцову, растворимых углеводов - фенольным методом. Установлено, что в листьях душицы сортообразцов 100.1 и № 1 через десять дней после обработки споровой культурой штамма B. thuringiensis 0271 количество хлорофиллов снижалось на 27,1 и 15,2% к контролю соответственно, а в листьях сортооб-разцов г-4и № 2 отмечали повышение их содержания на 91,4 и 72,7% соответственно. В листьях образца г-4на десятые сутки после обработки B. thuringiensis 0271 уменьшалось количество пролина и фенольных соединений в 4 раза. На десятые сутки после обработки штаммом 0271 сорто-образца 100.1 наблюдалось уменьшение количества растворимых углеводов в 1,76 раза к контролю и фенольных соединений в 2,0 раза. Обработка культурой штамма B. thuringiensis 0271 растений O. vulgare L. способствовала накоплению антиоксидантов в листьях образца г-4на десятые сутки на 14,5% к контролю.

In this paper, we aim to study the effect of the Bacillus thuriengiensis strain var. darmstadiensis 0271 on the biochemical parameters of Origanum vulgare L., which determine its nonspecific resistance to unfavourable environmental conditions. The research materials were a liquid spore culture of B. Thuringiensis 0271 and the following oregano samples: 100.1 with 75.5% of carvacrol, g-4with 52.0% of carvacrol, No. 2 with 59.85% of а-terpineol, No. 1 with 21.5% of germacrene D and 19.4% of в-caryophyllene. The preservation of the strain spores on the leaf surface of Origanum vulgare was determined using A.G. Kolchevskys method. The proline content was determined by V.A. Khramov’s and E.M. Ageeva’s method, while the pigment content was measured spectrophotometrically. The total content of phenolic compounds, ascorbic acid and water-soluble carbohydrates was established according to the methods by M.N. Zaprometov, M.M. Okuntsov and M. Dubois, respectively. Ten days after treating the leaves of the 100.1 and No. 1 oregano varieties with the spore culture of B. Thuringiensis 0271, the amount of chlorophylls decreased by 27.1 and 15.2% compared to the control, respectively. At the same time, the amount of chlorophylls increased by 91.4 and 72.7% in the leaves of the g-4and No. 2 varieties, respectively. On the 10th day of the experiment, the amount of proline and phenolic compounds decreased by 4 times in the leaves of the g-4 sample. Compared to the control, the 100.1 variety demonstrated a decrease in the amount of soluble carbohydrates and phenolic compounds by 1.76 and 2.0 times, respectively. On the 10th day of the experiment, the treatment of O. vulgare plants with B. Thuringiensis 0271 promoted the accumulation of antioxidants in the leaves of the g-4sample by 14.5% compared to the control.

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The authors declare that there are no conflicts of interest present.