vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-1-80-89vuzbiochemi-536Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВлияние синего света при культивировании мицелия Inonotus rheades на биологические свойства водных экстрактовImpact of blue light on the biological properties of aqueous extracts during the cultivation of the Inonotus rheades myceliumБоровскийГ. Б.BorovskiiG. B.

Боровский Геннадий Борисович, д.б.н., профессор, главный научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Gennadii B. Borovskii, Dr. Sci. (Biology), Professor, Chief Researcher

132, Lermontov St., Irkutsk, 664033

borovskii@sifibr.irk.ruГорностайТ. Г.GornostaiT. G.

Горностай Татьяна Геннадьевна, к.фарм.н., научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Tatyana G. Gornostai, Cand. Sc. (Pharmacy), Researcher

132, Lermontov St., Irkutsk, 664033

t.g.gornostay@yandex.ruПоляковаМ. С.PolyakovaM. S.

Полякова Марина Станиславовна, ведущий инженер

664033, г. Иркутск, ул. Лермонтова, 132

Marina S. Polyakova, Leading Engineer

132, Lermontov St., Irkutsk, 664033

poljakova.m@gmail.comБоровскаяМ. К.BorovskajaM. K.

Боровская Марина Казимировна, ведущий инженер

664033, г. Иркутск, ул. Лермонтова, 132

Marina K. Borovskaja, Leading Engineer

132, Lermontov St., Irkutsk, 664033

marbor1964@yandex.ruХаснатиновМ. А.KhasnatinovM. A.

Хаснатинов Максим Анатольевич, д.б.н., ведущий научный сотрудник

664033, г. Иркутск, ул. Тимирязева, 16

Maxim A. Khasnatinov, Dr. Sci. (Biology), Leading Research

16, Timiryazeva St., Irkutsk, 664033

khasnatinov@yandex.ruСоловаровИ. С.SolovarovI. S.

Соловаров Иннокентий Сергеевич, младший научный сотрудник

664033, г. Иркутск, ул. Тимирязева, 16

Innokentiy S. Solovarov, Junior Research

16, Timiryazeva St., Irkutsk, 664033

keschass@mail.ruДанчиноваГ. А.DanchinovaG. A.

Данчинова Галина Анатольевна, д.б.н., заведующая лабораторией трансмиссивных инфекций

664033, г. Иркутск, ул. Тимирязева, 16

Galina A. Danchinova, Dr. Sci. (Biology), Head of the Lab. of Vector-borne Infections

16, Timiryazeva St., Irkutsk, 664033

dan-chin@yandex.ruСибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and Biochemistry SB RASRussian FederationФедеральное государственное бюджетное научное учреждение «Научный центр проблем здоровья семьи и репродукции человека»РоссияFederal State Public Scientific Institution “Scientific Centre for Family Health and Human Reproduction Problems”Russian Federation2021050420211118089Copyright © Боровский Г.Б., Горностай Т.Г., Полякова М.С., Боровская М.К., Хаснатинов М.А., Соловаров И.С., Данчинова Г.А., 20212021Боровский Г.Б., Горностай Т.Г., Полякова М.С., Боровская М.К., Хаснатинов М.А., Соловаров И.С., Данчинова Г.А.Borovskii G.B., Gornostai T.G., Polyakova M.S., Borovskaja M.K., Khasnatinov M.A., Solovarov I.S., Danchinova G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/536

Цель работы – определить, зависят ли антиоксидантные, цитотоксические и вирулицидные свойства водных экстрактов из мицелия Inonotus rheades от условий освещения при его культивировании. Было изучено влияние освещения синим светом на мицелий I. rheades, который культивировали на древесине березы при температуре 25±1 ºС в темноте или при постоянном освещении синими светодиодами мощностью 12,8 Вт/м2. В ходе работы были получены две фракции водорастворимых полисахаридов: ВР-5 – выделенные из мицелия, выращенного при освещении синим светом; ВР-6 – выделенные из мицелия, выращенного в темноте. Экстракт из мицелия, выросшего в условиях освещения синим светом, проявлял большую антиоксидантную активность, чем экстракт из мицелия, выращенного в темноте. Исследование действия экстрактов на тестовую культуру опухолевых клеток показало, что экстракты вызывают небольшую гибель клеток на 6-е сутки совместной инкубации. Цитостатическое действие экстрактов также проявлялось спустя 6 суток. Плотность культуры при максимальных концентрациях уменьшалась до 60% от контроля для экстракта ВР-6 и до 20% – для экстракта ВР-5. Результаты измерения противовирусной активности экстрактов показали, что экстракты ВР-5 и ВР-6 полностью уничтожают вирус клещевого энцефалита. Экспериментально показано, что после нормализации значений рН экстрактов в обоих экстрактах присутствуют компоненты, проявляющие существенное противовирусное действие. Индекс ингибирования для экстракта BP-5 составил 3, а для BP-6 – 2 lg БОЕ/мл. Это позволяет предположить, что концентрация вирулицидных компонентов в экстракте из мицелия, выращенного в условиях освещения синим светом, приблизительно в 10 раз выше, чем в экстракте из мицелия, выращенного в темноте. Таким образом, в экстрактах из мицелия I. rheades, выращенного на дисках березы, содержатся вещества, имеющие антиоксидантные, цитостатические вирулицидные свойства. Их накопление стимулируется синим светом.

The aim was to determine whether the antioxidant, cytotoxic and virucidal properties of aqueous extracts isolated from the Inonotus rheades basidiomycete depend on the illumination of the mycelium during cultivation. Effects of blue light illumination on the mycelium of I. rheades, which was cultivated on birch wood at 25±1 ºC in the dark and under a constant illumination of 12.8 W/m2 were studied. In the course of the work, two fractions of water-soluble polysaccharides were obtained: ВР-5 – isolated from the mycelium grown under blue light; BP-6 – isolated from mycelium grown in the dark. Two fractions of water-soluble pol- ysaccharides were obtained during the study: ВР-5 – water-soluble polysaccharides isolated from the mycelium grown under blue light; ВР-6 – water-soluble polysaccharides isolated from the mycelium grown in the dark. The extract from the mycelium grown under blue light showed a greater antioxidant activity than that from the mycelium grown in the dark. An analysis of the effect of the extracts under study on a test culture of tumour cells showed that the extracts cause the death of some amount of the cells on the 6th day of coincubation. The cytostatic effect of the extracts was also manifested following 6 days. In comparison with the control, the density of the culture at maximum concentrations decreased to 60% and 20% for BP-6 and BP- 5, respectively. The results of measuring the antiviral activity of the extracts showed that BP-5 and BP-6 completely destroy tick-borne encephalitis viruses. It was experimentally shown that, after normalisation of the pH values, both extracts contain components exhibiting a significant antiviral effect. The inhibition index for BP-5 and BP-6 comprised 3 and 2 lg PFU/ ml, respectively. This suggests that the concentration of virucidal components in the extract from mycelium grown under blue light is approximately 10 times higher than that in the extract from the mycelium grown in the dark. Thus, the extracts from the mycelium of I. rheades grown on birch discs contain substances exhibiting antioxidant, cytostatic and virucidal properties. The accumulation of these properties can be stimulated by blue light illumination.

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The authors declare that there are no conflicts of interest present.