vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-1-90-98vuzbiochemi-537Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYОбразование первичных метаболитов и хлорофилла в растениях Cucumis sativus L. при воздействии конъюгата l-рамнозы с м-аминобензойной кислотойFormation of primary metabolites and chlorophyll in the plants of Cucumis sativus L. when exposed to l-ramnose conjugated with ma-aminobenzoic acidЧерепановИ. С.CherepanovI. S.

Черепанов Игорь Сергеевич, к.х.н., доцент

426034, г. Ижевск, ул. Университетская,1

Igor S. Cherepanov, Cand. Sci. (Chemistry), Associate Professor

1, Universitetskaya St., Izhevsk, 426034

cherchem@mail.ruЖуравлеваА. А.ZhuravlevaA. A.

Журавлева Алина Андреевна, студентка

426034, г. Ижевск, ул. Университетская,1

Alina A. Zhuravleva, Student

1, Universitetskaya St., Izhevsk, 426034

cherchem@mail.ruУдмуртский государственный университетРоссияUdmurt State UniversityRussian Federation2021050420211119098Copyright © Черепанов И.С., Журавлева А.А., 20212021Черепанов И.С., Журавлева А.А.Cherepanov I.S., Zhuravleva A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/537

Изучен характер влияния конъюгата L-рамнозы с м-аминобензойной кислотой на динамику образования первичных метаболитов и хлорофилла при проращивании семян огурца (Cucumis sativus L.). Синтетический аминоконъюгат (рамнозиламин) оказывает ингибирующее влияние на рост тест-растения, ослабевающее с уменьшением концентрации его раствора от 0,05 до 0,0005%. Анализ изменения профилей ИК-Фурье спектров образцов корней и гипокотилей пророщенных семян показывает наиболее существенные изменения в углеводном пуле и протеиновой составляющей биоматериала. С уменьшением содержания аминоконъюгата до 0,0005% происходит увеличение интенсивности полос 1060, 1100 и 1158 см-1, указывающее на накопление целлюлозных полисахаридов. Аналогичным образом меняется интенсивность амидных полос, при этом значения волновых чисел вторых производных спектральных сигналов в области 1600–1700 см-1 свидетельствуют о конформационных изменениях белков в процессе прорастания. Электронные спектры экстрактов и колебательные спектры образцов семядолей демонстрируют более интенсивное образование хлорофилла в системах с аминоконъюгатом, увеличивающееся с уменьшением содержания в среде последнего, при этом дополнительно свидетельствуют о различном состоянии фотосинтетических пигментов в контрольном и исследуемых образцах. По нашему предположению, влияние аминоконъюгата на параметры роста, образование первичных метаболитов и фотосинтетических пигментов обусловлено его присутствием в среде совместно с м-аминобензойной кислотой и L-рамнозой, образующимися в качестве продуктов гидролиза, протекание которого подтверждается поляриметрическими измерениями. Результаты исследований подтверждают перспективность поиска и испытаний соединений, содержащих в структуре активные компоненты разного спектра действия.

This article studies the effect of L-rhamnose conjugated with m-aminobenzoic acid on the formation of primary metabolites and chlorophyll during germination of cucumber (Cucumis sativus L.) seeds. This synthetic aminoconjugate (rhamnosylamine) has an inhibitory effect on the growth of the plant under study, which weakens with a decrease in the solution concentration from 0.05 to 0.0005%. An analysis of changes in the FTIR spectra of the root and hypocotyl samples of germinated seeds showed significant changes in the carbohydrate pool and protein structure of the biomaterial. As the aminoconjugate content decreases to 0.0005%, the intensity of the bands at 1060, 1100 and 1158 cm-1 increases, indicating the accumulation of cellulose polysaccharides. The intensity of the amide bands changes in a similar way, while the values of the wave numbers of the second derivatives of the spectral signals in the 1600-1700 cm-1 range indicate conformational changes in proteins during germination. The electronic spectra of extracts and the vibrational spectra of cotyledon samples demonstrated a more intense formation of chlorophyll in the systems comprising the aminoconjugate; the chlorophyll content increases with a decrease in the content of the ami- noconjugate in the medium. These spectra additionally indicate a different state of the photosynthetic pigments in the control and experimental samples. According to our assumption, the effect of the aminoconjugate on the growth parameters, the formation of primary metabolites and photosynthetic pigments can be explained by its presence in the medium simultaneously with m-aminobenzoic acid and L-rhamnose. The latter substances are formed as the products of hydrolysis, the course of which was confirmed by polarimetric measurements. The obtained results confirm the prospects of searching and testing compounds containing structural components of different action.

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The authors declare that there are no conflicts of interest present.