vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-1-107-115vuzbiochemi-539Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYОбразование фталатов при деградации N-фенил-2-нафтиламина почвенными бактериямиFormation of phthalates during the degradation of N-phenyl-2-naphthylamine by soil bacteriaМакароваЛ. Е.MakarovaL. E.

Макарова Людмила Евгеньевна, д.б.н., главный научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132

Lyudmila E. Makarova, Dr. Sci. (of Biology), Chief Researcher

132, Lermontov St., Irkutsk, 664033

makarova@sifibr.irk.ruМорицА. С.MoritsA. S.

Мориц Анна Сергеевна, ведущий инженер,

664033, г. Иркутск, ул. Лермонтова, 132

Anna S. Morits, Lead Engineer

132, Lermontov St., Irkutsk, 664033

СоколоваН. А.SokolovaN. A.

Соколова Наталья Александровна, ведущий технолог,

664033, г. Иркутск, ул. Лермонтова, 132

Natalia A. Sokolova, Lead Technologist

132, Lermontov St., Irkutsk, 664033, Russian Federation

Сибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and Biochemistry SB RASRussian Federation202105042021111107115Copyright © Макарова Л.Е., Мориц А.С., Соколова Н.А., 20212021Макарова Л.Е., Мориц А.С., Соколова Н.А.Makarova L.E., Morits A.S., Sokolova N.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/539

N-фенил-2-нафтиламин (N-ФНА) и фталаты относят к веществам антибиотического действия. Появление и накопление этих веществ в биосфере обусловлено их техногенным и биогенным происхождением (метаболиты растений и бактерий). Цель работы – сравнить деградирующую активность в отношении N-ФНА у почвенных бактерий Rhizobium leguminosarum bv. viceae, Bradyrhizobium japonicum, Pseudomonas syringae pv. pisi, Clavibacter michiganensis sps. sepedonicus, Azotobacter chroococcum, различающихся по типу взаимоотношений с растениями гороха (Pisum sativum L.), синтезирующего вышеназванное соединение. Продукты деградации исследовали методом газовой хромато-масс-спектрометрии в этилацетатных экстрактах из культуральных жидких сред, куда вместе с бактериями вносили N-ФНА до концентрации 10 мкМ. С применением методов высокоэффективной жидкостной хроматографии в полученных при помощи этилацетата экстрактах из культуральных сред, куда вносили N-ФНА до концентрации 100 мкМ, через двое суток роста бактерий в этих средах прослеживали степень уменьшения его концентрации. Показано, что все исследованные виды бактерий способны деградировать N-ФНА с образованием фталатов. Наиболее высокую деградирующую активность обнаружили у бактерий Rhizobium, эндосимбионтов растений гороха, синтезирующего N-ФНА, и у свободноживущих азотфиксирующих бактерий рода Azotobacter. N-ФНА снижал жизнеспособность всех видов бактерий, но в разной степени. В наибольшей мере негативное действие N-ФНА сказалось на жизнеспособности бактерий рода Azotobacter, показавшего при этом высокую деградирующую активность в отношении этого соединения. Зависимость эффекта негативного влияния на жизнеспособность от концентрации N-ФНА оказалась слабо выраженной у бактерий родов Rhizobium и Pseudomonas, а у бактерий родов Bradyrhizobium и Clavibacter она оказалась существенной.

N-phenyl-2-naphthylamine (N-PNA) and phthalates are classified as antibiotic substances. The appearance and accumulation of these substances in the biosphere is associated with their technogenic and biogenic origin (metabolites of plants and bacteria). In this article, we compare the degrading action of such soil bacteria as Rhizobium leguminosarum bv. viceae, Bradyrhizobium japonicum, Pseudomonas syringae pv. pisi, Clavibacter michiganensis sps. Sepedonicus and Azotobacter chroococcum against N-PNA. These bacteria differ in their interaction with pea plants (Pisum sativum L.) synthesising N-PNA. The degradation products were studied using gas chromatography-mass spectrometry in ethyl acetate extracts obtained from culture liquid media, in which N-PNA at a concentration of 10 μM and the bacteria under study were introduced. The decrease in the N-PNA concentration in the extracts obtained using ethyl acetate from culture media, in which N-PNA had been added to a concentration of 100 μM, was monitored following two days of bacterial growth using the methods of high-performance liquid chromatography. It was shown that all the studied bacterial species are capable of degrading N-PNA with the formation of phthalates. The Rhizobium bacteria, endosymbionts of pea plants synthesising N-PNA, and free-living nitrogen-fixing bacteria of the Azotobacter genus showed the highest degrading activity. It was found that N-PNA reduced the viability of all types of bacteria, although to a varying degree. N-PNA had the most negative effect on the viability of the Azotobacter genus, although these bacteria showed a high degrading action against N-PNA. The dependence between the negative effect of NPNA on bacterial viability and the N-PNA concentration was mildly pronounced for Rhizobium and Pseudomonas, although being significant for Bradyrhizobium and Clavibacter.

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The authors declare that there are no conflicts of interest present.