vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-1-125-135vuzbiochemi-541Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВлияние наноселена как компонента питательной среды на основные параметры культивирования и антагонистическую активность бактерий рода LactobacillusEffects of nanoselenium as a nutrient medium component on the main cultivation parameters and antagonistic activity of Lactobacillus strainsОмельченкоА. В.OmelchenkoA. V.

Омельченко Александр Владимирович, к.б.н., доцент кафедры ботаники и физиологии растений и биотехнологий

295007, г. Симферополь, пр-т Академика Вернадского, 4

Aleksandr V. Omelchenko, Cand. Sci. (Biology), Associate Professor, Department of Botany and Physiology of Plants and Biotechnology

4, Academician Vernadsky Ave., Simferopol, 295007

omelchenko_tnu@mail.ruРжевскаяВ. С.RzhevskayaV. S.

Ржевская Виктория Степановна, соискатель кафедры фармации

295051, г. Симферополь, б-р Ленина, 5/7

Victoria S. Rzhevskaya, candidate of the Department of Pharmacy

5/7, Lenin B/v, Simferopol, 295051

viktoriyar45@mail.ruКрыжкоА. В.KryzhkoA. V.

Крыжко Анастасия Владимировна, к.с-х.н., ведущий научный сотрудник лаборатории молекулярной генетики, протеомики и биоинформатики в сельском хозяйстве

295453, г. Симферополь, ул. Киевская, 150

Anastasiia V. Kryzhko, Cand. Sci. (Agriculture), Leading Researcher, Laboratory of Molecular Genetics, Proteomics and Bioinformatics in Agriculture Research

150, Kievskaya St., Simferopol, 295453

solanum@ukr.netПановД. А.PanovD. A.

Панов Денис Александрович, к.х.н., доцент кафедры общей и физической химии

295007, г. Симферополь, пр-т Академика Вернадского, 4

Denis A. Panov, Cand. Sci. (Chemistry), Associate Professor, Department of General and Physical Chemistry

4, Academician Vernadsky Ave., Simferopol, 295007

panovda@cfuv.ruБугараИ. А.BugaraI. A.

Бугара Игорь Александрович, к.б.н., доцент кафедры ботаники и физиологии растений и биотехнологий

295007, г. Симферополь, пр-т Академика Вернадского, 4

Igor A. Bugara, Cand Sci. (Biology), Associate Professor, Department of Botany and Physiology of Plants and Biotechnology

4, Academician Vernadsky Ave., Simferopol, 295007

bia.05@mail.ruКрымский федеральный университет им. В.И. ВернадскогоРоссияV.I. Vernadsky Crimean Federal UniversityRussian FederationНаучно-исследовательский институт сельского хозяйства КрымаРоссияInstitute of Agriculture of CrimeaRussian Federation202105042021111125135Copyright © Омельченко А.В., Ржевская В.С., Крыжко А.В., Панов Д.А., Бугара И.А., 20212021Омельченко А.В., Ржевская В.С., Крыжко А.В., Панов Д.А., Бугара И.А.Omelchenko A.V., Rzhevskaya V.S., Kryzhko A.V., Panov D.A., Bugara I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/541

Целью исследования являлось установление влияния наноселена на динамику роста и антагонистическую активность штаммов Lactobacillus casei IMB B-7343 и Lactobacillus plantarum IMB B-7344 относительно фитопатогенной бактерии Xanthomonas campestris В-4102. Наночастицы селена получали путем восстановления раствора селенита натрия L-цистеином в присутствии альгината натрия. Культивирование лактобактерий проводили на питательной среде MRS с добавлением коллоидного раствора наноселена в концентрации: 0,05; 0,1; 0,15; 0,2 и 0,25 мг/л (по селену). Бактерии культивировали в 96-луночном планшете в фотометре Multiskan FC при 36 ºС в режиме постоянного встряхивания. Антагонистическую активность лактобактерий изучали методом агаровых блоков. Установлено, что добавление в питательную среду наноселена в концентрации от 0,2 до 0,25 мг/л способствовало сокращению длительности экспоненциальной фазы роста в среднем на 3 ч и уменьшению накопления биомассы штамма L. casei IMB B-7343 на 15,0%. Показано увеличение оптической плотности культуры этого штамма на 15,9% в стационарной фазе роста в варианте с добавлением к питательной среде раствора наноселена в концентрации 0,15 мг/л. Отмечено, что при культивировании штамма L. plantarum IMB B-7344 в питательной среде MRS с добавлением коллоидного раствора наноселена в концентрации 0,05–0,25 мг/л в фазу экспоненциального развития наблюдалось ускорение роста культуры в среднем на 5 ч относительно контроля. Отмечено, что при добавлении в питательную среду наноселена наблюдалось увеличение антагонистической активности штаммов лактобактерий по отношению к фитопатогенной бактерии X. campestris В-4102, особенно у штамма L. plantarum IMB B-7344 (зона подавления роста фитопатогена составила 14,5–15 мм).

The study aims to establish the effect of nanoselenium on the growth and antagonistic activity of the Lactobacillus casei IMB B-7343 and Lactobacillus plantarum IMB B-7344 strains against the phytopathogenic bacterium Xanthomonas campestris B-4102. Selenium nanoparticles were obtained by reducing a solution of sodium selenite with L-cysteine in the presence of sodium alginate. The cultivation of Lactobacilliwas carried out in a MRS nutrient medium with the addition of a colloidal solution of nanoselenium at the following concentrations: 0.05; 0.1; 0.15; 0.2 and 0.25 mg/l (for selenium). Bacteria were cultured in a 96-well plate in a Multiskan FC photometer at 36 ºС under constant shaking. The antagonistic activity of the strains was studied by the method of agar blocks. It was found that the addition of nanoselenium to the nutrient medium at a concentration of 0.2 to 0.25 mg/l contributed to a decrease in the duration of the exponential growth phase by an average of 3 hours, as well as to a decrease in the biomass accumulation of the L. casei IMB B-7343 strain by 15.0%. The optical density of this strain culture was observed to increase by 15.9% in the stationary growth phase under the addition of a nanoselenium solution to the nutrient medium at a concentration of 0.15 mg/l. During the cultivation of the L. plantarum IMB B-7344 strain in an MRS nutrient medium with the addition of a colloidal solution of nanoselenium at a concentration of 0.05–0.25 mg/l in the exponential development phase, an acceleration of the culture growth on average by 5 hours relative to the control was observed. It was noted that, upon the addition of nanoselenium to the nutrient medium, an increase in the antagonistic activity of lactobacillus strains against the phytopathogenic bacterium X. campestris B-4102 was observed, particularly in the L. plantarum IMB B-7344 strain (the zone of inhibition of the phytopathogen growth was 14.5–15 mm).

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The authors declare that there are no conflicts of interest present.