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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vuzbiochemi</journal-id><journal-title-group><journal-title xml:lang="ru">Известия вузов. Прикладная химия и биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of Universities. Applied Chemistry and Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-2925</issn><issn pub-type="epub">2500-1558</issn><publisher><publisher-name>ИРНИТУ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21285/2227-2925-2021-11-2-228-235</article-id><article-id custom-type="elpub" pub-id-type="custom">vuzbiochemi-599</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PHYSICOCHEMICAL BIOLOGY</subject></subj-group></article-categories><title-group><article-title>Влияние гумусовых кислот низинного торфа на ремедиационные свойства растений пшеницы при комплексном загрязнении тяжелыми металлами</article-title><trans-title-group xml:lang="en"><trans-title>Influence of humic acids in lowland peat on the remediation properties of wheat plants against heavy metal contamination</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кирдей</surname><given-names>Т. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kirdey</surname><given-names>T. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кирдей Татьяна Александровна – кандидат биологических наук, доцент кафедры агрономии и агробизнеса.</p><p>153012, Иваново, ул. Советская, 45.</p></bio><bio xml:lang="en"><p>Tatiana A. Kirdey - Cand. Sci. (Biology), Associate Professor, Agronomy and Agrobusiness Department, Ivanovo State Agricultural Academy by D.K. Belyaev.</p><p>45, Sovetskaya St., Ivanovo, 153012.</p></bio><email xlink:type="simple">t.a.kirdey@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Ивановская государственная сельскохозяйственная академия им. Д.К. Беляева</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Ivanovo State Agricultural Academy by D.K. Belyaev</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>03</day><month>07</month><year>2021</year></pub-date><volume>11</volume><issue>2</issue><fpage>228</fpage><lpage>235</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кирдей Т.А., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Кирдей Т.А.</copyright-holder><copyright-holder xml:lang="en">Kirdey T.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vuzbiochemi.elpub.ru/jour/article/view/599">https://vuzbiochemi.elpub.ru/jour/article/view/599</self-uri><abstract><p>Фиторемедиация является перспективной технологией очистки почвы и воды от тяжелых металлов. Несмотря на свидетельства повышения накопления тяжелых металлов культурными растениями под влиянием природных комплексообразователей - гумусовых кислот, их эффективность в фиторемедиации остается малоизученной. В связи с этим цель настоящей работы состояла в выяснении особенностей влияния препарата гумусовых кислот торфа на ремедиационный потенциал растений пшеницы (Triticum aestivum L.) при комплексном загрязнении тяжелыми металлами. Влияние полиметаллического загрязнения на ремедиационные свойства пшеницы изучали в модельных вегетационных экспериментах с использованием водной культуры. Растения выращивали на питательном растворе Хогланда. Комплексное действие тяжелых металлов создавали при использовании 10 мкмоль/л CdSO4, 25 и 50 мкмоль/л CuSO4, 500 и 1000 мкмоль/л Pb(NO3)2 в различных сочетаниях с добавлением препарата гумусовых кислот торфа (0,005%) или без него. Фиторемедиационную эффективность препарата гумусовых кислот определяли по выносу тяжелых металлов в фазу колошения пшеницы. В результате исследований установлено, что фиторемедиационная эффективность препарата гумусовых кислот определяется как усилением поглощения тяжелых металлов, так и снижением их токсического действия на растения. При комплексном загрязнении раствора тяжелыми металлами, которое характеризовалось высокой токсичностью, в вариантах с добавлением гумусовых кислот накопление растениями пшеницы меди и кадмия повысилось в 1,2-2,5 раза. Данные свидетельствуют о возможности использования препарата гумусовых кислот торфа в фиторемедиационных технологиях в качестве эффектора фитоэкстракции тяжелых металлов.</p></abstract><trans-abstract xml:lang="en"><p>Phytoremediation is a promising technology for removing heavy metals from soil and water. Despite the pronounced increase in heavy metal accumulation by cultivated plants under the influence of naturally occurring complexing agents, such as humic acids, their efficiency in phytoremediation has been poorly studied. In this regard, the aim of this work is to elucidate the effect of peat humic acid formulations on the remediation potential of wheat plants (Triticum aestivum L.) against heavy metal contamination. The influence of polymetallic pollution on the remediation properties of wheat was studied in model vegetation experiments using a culture solution. Plants were grown in a Hoagland nutrient solution. A complex exposure to heavy metals was simulated using 10 pmol/L CdSO4, 25 and 50 pmol/L CuSO4, 500 and 1000 pmol/L Pb(NO3)2 in various combinations with or without the addition of a peat humic acid formulation (0.005%).</p><p>The phytoremediation efficiency of the humic acid formulation was determined by the removal of heavy metals during the heading stage of wheat growth. The research results showed that the phytoremediation efficiency of the humic acid formulation is defined by both an increase in the absorption of heavy metals and a decrease in their toxic action on the plants. In the case of mixed contamination of the solution with highly toxic heavy metals, the samples with humic acids showed a 1.2-2.5-fold increase in the accumulation of copper and cadmium by wheat plants. The data demonstrates the possibility of using the formulation of peat humic acids in phytoremediation technologies as an effector of heavy metal phytoextraction.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>пшеница</kwd><kwd>гумусовые кислоты</kwd><kwd>тяжелые металлы</kwd><kwd>свинец</kwd><kwd>кадмий</kwd><kwd>медь</kwd><kwd>фиторемедиация</kwd></kwd-group><kwd-group xml:lang="en"><kwd>wheat</kwd><kwd>humic acids</kwd><kwd>heavy metals</kwd><kwd>lead</kwd><kwd>cadmium</kwd><kwd>copper</kwd><kwd>phytoremediation</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Lyanguzova I.V. Dynamic trends of heavy metal contents in plants and soil under different industrial air pollution regimes // Russian Journal of Ecology. 2017. Vol. 48. Issue 4. P. 311-320. https: //doi.org/10.1134/S1067413617040117</mixed-citation><mixed-citation xml:lang="en">Lyanguzova IV. Dynamic trends of heavy metal contents in plants and soil under different industrial air pollution regimes. Russian Journal of Ecology. 2017;48(4):311-320. https://doi.org/10.1134/S1067413617040117</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Гиниятуллин Р.Х., Бактыбаева З.Б. Особенности накопления Cd и Ni лиственницей Сукачева (Larix sukaczewii Dyl.) в условиях техногенеза // Вестник Томского государственного университета. Биология. 2020. N 51. C. 141-161. https://doi.org/10.17223/19988591/51/8</mixed-citation><mixed-citation xml:lang="en">Giniyatullin RH, Baktybaeva ZB. Features of Cd and Ni accumulation by Larix sukaczewii Dyl. under technogenesis. Vestnik Tomskogo Gosudarstvennogo Universiteta. Biologiya = Tomsk State University Journal of Biology. 2020;51:141-161. (In Russian) https://doi.org/10.17223/19988591/51/8</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Кабата-Пендиас А., Пендиас Х. Микроэлементы в почвах и растениях / пер. с англ. Д.В. Гричука, Е.П. Янина; под ред. Ю.Е. Саета. М.: Мир, 1989. 439 с.</mixed-citation><mixed-citation xml:lang="en">Kabata-Pendias A, Pendias Kh. Trace elements in soils and plants. Moscow: Mir; 1989, 439 p. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Prasad M.N.V. Phytoremediation of metal-polluted ecosystems: hype for commercialization // Russian Journal of Plant Physiology. 2003. Vol. 50. Issue 5. Р. 686-701. https://doi.org/10.1023/A:1025604627496</mixed-citation><mixed-citation xml:lang="en">Prasad MNV. Phytoremediation of metal-polluted ecosystems: hype for commercialization. Russian Journal of Plant Physiology. 2003;50(5): 686-701. https://doi.org/10.1023/A:1025604627496</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Baker A.J.M. Accumulators and excluders -strategies in the response of plants to heavy metals // Journal of Plant Nutrition. 1981. Vol. 3. Issue 1-4. P. 643-654. https://doi.org/10.1080/01904168109362867</mixed-citation><mixed-citation xml:lang="en">Baker AJM. Accumulators and excluders -strategies in the response of plants to heavy metals. Journal of Plant Nutrition. 1981;3(1-4):643-654. https://doi.org/10.1080/01904168109362867</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Pilon-Smits E. Phytoremediation // Annual Review of Plant Biology. 2005. Vol. 56. P. 15-39. https://doi.org/10.1146/annurev.arplant.56.032604.144214</mixed-citation><mixed-citation xml:lang="en">Pilon-Smits E. Phytoremediation. Annual Review of Plant Biology. 2005;56:15-39. https://doi.org/10.1146/annurev.arplant.56.032604.144214</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Prieto M.J., Acevedo SOA, Prieto G.F., Nallely T.G. Phytoremediation of soils contaminated with heavy metals // Biodiversity International Journal. 2018. Vol. 2. Issue 4. P. 362-376. https://doi.org/10.15406/bij.2018.02.00088</mixed-citation><mixed-citation xml:lang="en">Prieto MJ, Acevedo SOA, Prieto GF, Nallely TG. Phytoremediation of soils contaminated with heavy metals. Biodiversity International Journal. 2018;2(4):362-376. https://doi.org/10.15406/bij.201 8.02.00088</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Yan A., Wang Y., Tan S.N., Mohd Yusof M.L., Ghosh S., Chen Z. Phytoremediation: A promising approach for revegetation of heavy metal-polluted land // Frontiers in Plant Science. 2020. Vol. 11. Р. 359. https://doi.org/10.3389/fpls.2020.00359</mixed-citation><mixed-citation xml:lang="en">Yan A, Wang Y, Tan SN, Mohd Yusof ML, Ghosh S, Chen Z. Phytoremediation: A promising approach for revegetation of heavy metal-polluted land. Frontiers in Plant Science. 2020;11:359. https://doi.org/10.3389/fpls.2020.00359</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Evangelou M.W.H., Robinson B.H., Gunthardt-Goerg M.S., Schulin R. Metal uptake and allocation in trees grown on contaminated land: implications for biomass production // International Journal of Phytoremediation. 2012. Vol. 15. Issue 1. P. 77-90. https://doi.org/10.1080/15226514.2012.670317</mixed-citation><mixed-citation xml:lang="en">Evangelou MWH, Robinson BH, Gunthardt-Goerg MS, Schulin R. Metal uptake and allocation in trees grown on contaminated land: implications for biomass production. International Journal of Phytoremediation. 2012;15(1):77-90. https://doi.org/10.1080/15226514.2012.670317</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Salt D.E., Blaylock M., Kumar N.P., Dushenkov V., Ensley B.D., Chet I., Raskin I. Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants // Biotechnology. 1995. Vol. 13. Issue 5. P. 468-475. https://doi.org/10.1038/nbt0595-468</mixed-citation><mixed-citation xml:lang="en">Salt DE, Blaylock M, Kumar NP, Dushenkov V, Ensley BD, Chet I, Raskin I. Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants. Biotechnology. 1995; 13(5):468-475. https://doi.org/10.1038/nbt0595-468</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Vamerali T., Bandiera M., Mosca G. Field crops for phytoremediation of metal-contaminated land. A review // Environmental Chemistry Letters. 2010. Vol. 8. Issue 1. P. 1-17. https://doi.org/10.1007/s10311-009-0268-0</mixed-citation><mixed-citation xml:lang="en">Vamerali T, Bandiera M, Mosca G. Field crops for phytoremediation of metal-contaminated land. A review. Environmental Chemistry Letters. 2010;8(1):1-17. https://doi.org/10.1007/s10311-009-0268-0</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Lee M., Yang M. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater // Journal of Hazardous Materials. 2010. Vol. 173. Issue 1-3. P. 589-596. https://doi.org/10.1016/j.jhazmat.2009.08.127</mixed-citation><mixed-citation xml:lang="en">Lee M, Yang M. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater. Journal of Hazardous Materials. 2010;173(1-3):589-596. https://doi.org/10.1016/j.jhazmat.2009.08.127</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Jensen J.K., Holm P.E., Nejrup J., Borggaard O.K. A laboratory assessment of potentials and limitations of using EDTA, rhamnolipids, and compost-derived humic substances (HS) in enhanced phytoextraction of copper and zinc polluted calcareous soils // Soil and Sediment Contamination: an International Journal. 2011. Vol. 20. Issue 7. P. 777-789. https://doi.org/10.1080/15320383.2011.609198</mixed-citation><mixed-citation xml:lang="en">Jensen JK, Holm PE, Nejrup J, Borggaard OK. A laboratory assessment of potentials and limitations of using EDTA, rhamnolipids, and compost-derived humic substances (HS) in enhanced phytoextraction of copper and zinc polluted calcareous soils. Soil and Sediment Contamination: an International Journal. 2011;20(7):777-789. https://doi.org/10.1080/15320383.2011.609198</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Halim M., Conte P., Piccolo A. Potential availability of heavy metals to phytoextraction from contaminated soils induced by exogenous humic substances // Chemosphere. 2003. Vol. 52. Issue 1. P. 265-275. https://doi.org/10.1016/S0045-6535(03)00185-1</mixed-citation><mixed-citation xml:lang="en">Halim M, Conte P, Piccolo A. Potential availability of heavy metals to phytoextraction from contaminated soils induced by exogenous humic substances. Chemosphere. 2003;52(1):265-275. https://doi.org/10.1016/S0045-6535(03)00185-1</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Evangelou M.W.H., Daghan H., Schaeffer A. The influence of humic acids on the phytoextraction of cadmium from soil // Chemosphere. 2004. Vol. 57. Issue 3. P. 207-213. https://doi.org/10.1016/j.chemosphere.2004.06.017</mixed-citation><mixed-citation xml:lang="en">Evangelou MWH, Daghan H, Schaeffer A. The influence of humic acids on the phytoextraction of cadmium from soil. Chemosphere. 2004;57(3):207-213. https://doi.org/10.1016/j.chemosphere.2004.06.017</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Кирдей Т.А. Влияние гумата на фиторемедиационные свойства пшеницы при возрастающих концентрациях нитрата свинца // Известия вузов. Прикладная химия и биотехнология. 2017. Т. 7. N 4. С. 110-115. https://doi.org/10.21285/2227-2925-2017-7-4-110-115</mixed-citation><mixed-citation xml:lang="en">Kirdey TA. The influence of humate on the phytoremediation properties of wheat with increasing concentrations of lead nitrate. Izvestiya Vuzov. Prikladnaya Khimiya i Biotekhnologiya = Proceedings of Universities. Applied Chemistry and Biotechnology. 2017;7(4):110-115. (In Russian) https://doi.org/10.21285/2227-2925-2017-7-4-110-115</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Чураков А.А. Запасы торфа в России // Лесной вестник. 2003. N 3. С. 22-25.</mixed-citation><mixed-citation xml:lang="en">Churakov AA. Peat reserves in Russia. Lesnoi vestnik = Forestry bulletin. 2003;3:22-25. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Пат. № 2310633, Российская Федерация. Способ получения жидких торфяных гуматов / Ю.А. Калинников, И.Ю. Вашурина, Т.А. Кирдей; патентообладатель ООО «Научно-производственная фирма “Недра”»; заявл. 15.06.2006; опубл. 20.11.2007. Бюл. № 32. 4 с.</mixed-citation><mixed-citation xml:lang="en">Kalinnikov JA, Vashurina IJ, Kirdej TA. Method for production of liquid peat humates. Patene RF, no. 2310633; 2006. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Hoaglond DR, Arnon DE. The water-culture method for growing plants without soil. California Agriculture Experimental Station. 1950. Available from: https://ia800306.us.archive.org/6/items/watercultureme3450hoag/watercultureme3450hoag.pdf [Accessed 25th Novemder 2020].</mixed-citation><mixed-citation xml:lang="en">Hoaglond DR, Arnon DE. The water-culture method for growing plants without soil. California Agriculture Experimental Station. 1950. Available from: https://ia800306.us.archive.org/6/items/wa-tercultureme3450hoag/watercultureme3450hoag.pdf [Accessed 25th Novemder 2020].</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
