vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-2-260-271vuzbiochemi-603Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYИсследование показателей безопасности экстрактов каллусных культур Pulmonaria officinalis и их фитохимического состава на наличие биологически активных веществ с потенциальными геропротекторными свойствамиStudy on safety profile in extracts of Pulmonaria officinalis callus cultures and their phytochemical composition for the presence bioactive substances with the potential geroprotective propertiesДышлюкЛ. С.DushlyukL. S.

Дышлюк Любовь Сергеевна – кандидат биологических наук, доцент кафедры бионанотехнологии.

650000, Кемерово, ул. Красная, 6.

Lubov S. Dushlyuk - Cand. Sci. (Biology), Associate Professor, Department of Bionanotechnology, Kemerovo State University.

6, Krasnaya St., Kemerovo, 650000.

soldatovals1984@mail.ruДроздоваМ. Ю.DrozdovaM. Yu.

Дроздова Маргарита Юрьевна - магистрант, лаборант-исследователь лаборатории биотестирования природных нутрицевтиков.

650000, Кемерово, ул. Красная, 6.

Margarita Yu. Drozdova - Master Student, Researcher, Natural Nutraceutical Bioassay Laboratory, Kemerovo State University.

6, Krasnaya St., Kemerovo, 650000.

drozdowa.margarita.00@yandex.ruЛосеваА. И.LosevaA. I.

Лосева Анна Ивановна – кандидат технических наук, начальник Центра научной периодики.

650000, Кемерово, ул. Красная, 6.

Anna I. Loseva - Cand. Sci. (Engineering), Head of the Department of Scientific and Publishing Activities, Kemerovo State University.

6, Krasnaya St., Kemerovo, 650000.

unid.kemsu@mail.ruКемеровский государственный университетРоссияKemerovo State UniversityRussian Federation202104072021112260271Copyright © Дышлюк Л.С., Дроздова М.Ю., Лосева А.И., 20212021Дышлюк Л.С., Дроздова М.Ю., Лосева А.И.Dushlyuk L.S., Drozdova M.Y., Loseva A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/603

Старение является естественным и неизбежным процессом, сопровождающимся различными заболеваниями. Растение медуница лекарственная (Pulmonaria officinalis) является источником биологически активных веществ, способных замедлять процессы старения и улучшать качество жизни людей. Каллусная культура данного растения может быть перспективным сырьем для создания нутрицевтиков. Однако состав медуницы лекарственной по наличию индивидуальных биологически активных веществ недостаточно изучен, особенно в отношении фенольных соединений с потенциальными геропротекторными свойствами. Проведено исследование фитохимического состава экстракта каллусных культур медуницы лекарственной на наличие биологически активных веществ с потенциальными геропротекторными свойствами и определены показатели его качества. Двукратную экстракцию каллусных культур Pulmonaria officinalis проводили на водяной бане с 70%-м этиловым спиртом. Определение показателей безопасности экстрактов проводили согласно требованиям фармакопейной статьи. К важным показателям качества экстрактов относили органолептические, физико-химические и микробиологические свойства. Продемонстрировано, что содержание тяжелых металлов, радионуклидов, сухой остаток, остаточное содержание спирта, а также органолептические показатели и микробиологическая чистота соответствуют нормативным документам. Фитохимический состав экстракта каллусных культур определяли методами высокоэффективной жидкостной и трехслойной хроматографии. Идентифицированы флавоноиды (рутин, изорамнетин, кверцетин), тритерпеновые сапонины и фенольные кислоты (п-кумаровая, феруловая, галловая, кофейная, розмариновая и хлорогеновая). Наличие тритерпенового сапонина, п-кумаровой, феруловой и галловой кислот обнаружено в экстракте каллусной культуры медуницы впервые. Количественный анализ биологически активных веществ показал, что содержание кофейной, розмариновой и хлорогеновой кислот в экстракте каллусной культуры является более высоким, чем содержание этих же веществ в экстрактах из надземных частей растения.

Ageing is a natural and inevitable process accompanied by different diseases. Medicinal lungwort (Pulmonaria officinalis) is a source of bioactive substances that can slow down ageing and improve the quality of human life. Callus culture of this plant can be a promising raw material to produce nutraceuticals. However, a composition of individual bioactive substances in medicinal lungwort has been studied insufficiently, particularly when it comes to phenolic compounds with potential geroprotective properties. We performed a study on the phytochemical composition of the callus cultures, extracted from the medicinal lungwort plant, for the presence of bioactive substances with the potential geroprotective properties and determined their quality profile. Twofold extraction of Pulmonaria officinalis callus cultures was carried out on a water bath with 70% ethyl alcohol. Determination of the safety profile of extracts was performed according to the requirements of the Pharmaceutical Norms and Regulations. Organoleptic, physicochemical and microbiological properties were considered important indicators of the quality of the extracts. It was demonstrated that the content of heavy metals, radionuclides, dry residue, residual alcohol content, as well as organoleptic characteristics and microbiological purity, comply with the regulatory documents. The phytochemical composition of the callus culture extract was determined by high-performance liquid chromatography and threelayer chromatography. The following flavonoids (rutin, isorhamnetin, quercetin), triterpenoid saponins and phenolic acids (p-coumaric, ferulaic, gallic, caffeic, rosmarinic and chlorogenic) were identified. The presence of triterpenoid saponin, p- coumaric, ferulaic and gallic acids is discovered in the extract of the lungwort callus culture for the first time. Quantitative analysis of bioactive substances showed that the content of caffeic, rosmarinic and chlorogenic acids in the callus culture extract is higher than that in the extracts from the aerial parts of the plant.

медуница лекарственнаякаллусные культурыбиологически активные веществагеропротекторыфенольные кислотыфитохимический составпоказатели безопасностиmedicinal lungwortcallus culturesbioactive substancesgeroprotectorsphenolic acidsphytochemical compositionsafety profileРабота выполнена при финансовой поддержке Министерства науки и высшего образования РФ (проект FZSR-2020-0006 ««Скрининг биологически активных веществ растительного происхождения, обладающих геропротекторными свойствами, и разработка технологии получения нутрицевтиков, замедляющих старение».This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project FZSR-2020-0006 "Screening of biologically active substances of plant origin with geroprotective properties, and development of technology for obtaining nutraceuticals that slow down aging").ReferencesPrasanth M.I., Sivamaruthi B.S., Kesika P., Rosmol P.S., Tencomnao T. 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The authors declare that there are no conflicts of interest present.