vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-2-281-289vuzbiochemi-605Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYХарактеристика полисахаридов из корней Eremurus hissaricus методом ИК-Фурье спектроскопииCharacterization of polysaccharides from Eremurus hissaricus roots by FTIR spectroscopyАшуровА. И.AshurovA. I.

Ашуров Ашурбой Ильхомович – докторант.

734063, Душанбе, ул. Айни, 299/2.

Ashurboy I. Ashurov - Doctoral Student, V.I. Nikitin Institute of Chemistry, National Academy of Sciences of Tajikistan.

299/2, Aini St., Dushanbe, 734063.

ashurboy_1593@mail.ruДжонмуродовА. С.DzhonmurodovA. S.

Джонмуродов Абдували Саломович – кандидат химических наук, ведущий научный сотрудник лаборатории химии высокомолекулярных соединений.

734063, Душанбе, ул. Айни, 299/2.

Abduvali S. Dzhonmurodov - Cand. Sci (Chemistry), Leading Researcher Laboratory of Biopolymers, V.I. Nikitin Institute of Chemistry, National Academy of Sciences of Tajikistan.

299/2, Aini St., Dushanbe, 734063.

abduvalid@mail.ruУсмановаС. Р.UsmanovaS. R.

Усманова Сураё Рахматжановна - кандидат химических наук, ведущий научный сотрудник лаборатории химии высокомолекулярных соединений,

734063, Душанбе, ул. Айни, 299/2.

Surayo R. Usmanova - Cand. Sci (Chemistry), Leading Researcher, Laboratory of Biopolymers, V.I. Nikitin Institute of Chemistry, National Academy of Sciences of Tajikistan.

299/2, Aini St., Dushanbe, 734063.

surayo.usmanova@gmail.comХоловШ. Е.KholovSh. E.

Холов Шавкат Ерович - кандидат технических наук, старший научный сотрудник лаборатории химии высокомолекулярных соединений,

734063, Душанбе, ул. Айни, 299/2.

Shavkat E. Kholov - Cand. Sci. (Engineering), Senior Scientist, Laboratory of Biopolymers, V.I. Nikitin Institute of Chemistry, National Academy of Sciences of Tajikistan.

299/2, Aini St., Dushanbe, 734063.

shavkat.kholov@yandex.ruМухидиновЗ. К.MuhidinovZ. M.

Мухидинов Зайниддин Камарович - доктор химических наук, профессор, главный научный сотрудник лаборатории химии высокомолекулярных соединений.

734063, Душанбе, ул. Айни, 299/2.

Zainiddin K. Muhidinov - Dr. Sci. Chemistry), Professor, Chief Researcher, Laboratory of Biopolymers, V.I. Nikitin Institute of Chemistry, National Academy of Sciences of Tajikistan.

299/2, Aini St., Dushanbe, 734063.

zainy@mail.ruИнститут химии им В.И. Никитина НАН ТаджикистанаТаджикистанV.I. Nikitin Institute of Chemistry, National Academy of Sciences of TajikistanTajikistan202104072021112281289Copyright © Ашуров А.И., Джонмуродов А.С., Усманова С.Р., Холов Ш.Е., Мухидинов З.К., 20212021Ашуров А.И., Джонмуродов А.С., Усманова С.Р., Холов Ш.Е., Мухидинов З.К.Ashurov A.I., Dzhonmurodov A.S., Usmanova S.R., Kholov S.E., Muhidinov Z.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/605

Структура и характеристика водорастворимых глюко- и галактоманнанов (GlcMan and GalMan), выделенных из корней нескольких родственных видов растения Eremurus, были изучены ранее. В этой работе дана характеристика полисахаридов и других продуктов экстракции из корнеклубней растения эремуруса гиссарского (Eremurus hissaricus) методом инфракрасной Фурье (ИК-Фурье) спектроскопии с помощью приставки нарушенного полного внутреннего отражения. Образцы полисахаридов были очищены от белковых веществ методом Севаджа, обесцвечены через полиамидную колонку. В ИК-Фурье спектрах основные пики, отнесенные к асимметричным и симметричным валентным колебаниям CH2 пиранозного кольца (Для GluMan - 2886, 1373, 1244 см-1; Для GalGluMan - 2923, 1370, 1238 см-1), усиливаются при очистке после удаления связанных белковых примесей. При анализе спектров образца очищенного GalGluMan в изученных полисахаридах был использован пакет прикладных программ KnownitAll и IRPal 2. Обнаружено, что интенсивность максимума поглощения уменьшалась при 1732 см-1, в то же время при 1552,92 см-1 сформировалась новая полоса, относящаяся к валентным колебаниям карбоксильных (CO) или нитрозо- (NO) групп в очищенном полисахариде, она образовалась в результате реакции Майяра между белком и восстанавливающим концом полисахаридного звена. Интенсивность полос в области 1238-1244 см-1 также уменьшилась в очищенных образцах, что, возможно, связано с отщеплением небольшой фракции белка. Показано, что применение ИК-Фурье спектроскопии помогло быстро и качественно охарактеризовать процесс очистки водорастворимого глюкоманнана (GluMan) и растворимого в кислоте полисахарида глюкогалактоманнана (GalGluMan) от примесей акриламида и позволило сравнительно оценить состояние функциональных групп у данных полисахаридов корнеклубней растения эремуруса гиссарского (E. hissaricus).

The structure of water'soluble gluco' and galactomannans (GlcMan and GalMan) isolated from the roots of several Eremurus'related plant species has been studied previously. This article characterizes polysaccharides and other extraction products from the root tubers of Eremurus hissaricus by Fourier' transform infrared (FTIR) spectroscopy using an attenuated total reflectance (ATR) accessory. Polysaccharide samples were purified from protein substances with the Sevage method and discoloured through a polyamide column. In the FTIR spectra, the main peaks attributed to asymmetric and symmetric stretching vibrations of CH2 of the pyranose ring (for GluMan - 2886, 1373, 1244 cm-1; for GalGluMan - 2923, 1370, 1238 cm-1) were enhanced by purification after removing bound protein impurities. The KnownitAll and IR-Pal 2 software applications were used to examine the spectra of a sample of purified GalGluMan in the studied polysaccharides. The results show that the intensity of the absorption maximum decreased at 1732 cm-1. At the same time, at 1552.92 cm-1, a new band appeared that refers to the valence vibrations of carboxyl (CO) or nitroso (NO) groups in the purified polysaccharide. This band appeared as a result of the Maillard reaction between the protein and the reducing end of the polysaccharide unit. The intensity of the bands in the 1238-1244 cm-1 region also decreased in the purified samples, which may be due to the elimination of a small fraction of protein. The use of FTIR spectroscopy allows the process of purifying watersoluble GluMan and acid-soluble polysaccharide of GalGluMan from acrylamide impurities to be characterized timely and accurately. Additionally, this method allows a comparative estimation of functional groups in polysaccharides of the root tubers of E. hissaricus.

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The authors declare that there are no conflicts of interest present.