vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2021-11-3-403-412vuzbiochemi-648Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYЭкзогенный кальций модулирует активность аденилатциклаз растений картофеля при биотическом стрессеExogenous calcium modulates the activity of adenylate cyclases in potato plants under biotic stressФилиноваН. В.FilinovaN. V.

Филинова Надежда Владимировна, к.б.н., научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132, а/я 317

Nadegda V. Filinova, Cand. Sci. (Biology), Researcher

132, Lermontov St., Irkutsk, 664033

filinova@sifibr.irk.ruЛомоватскаяЛ. А.LomovatskayaL. A.

Ломоватская Лидия Арнольдовна, д.б.н., профессор, ведущий научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132, а/я 317

Lidiya A. Lomovatskaya Dr. Sci. (Biology), Professor, Leading Researcher

132, Lermontov St., Irkutsk, 664033

LidaL@sifibr.irk.ruРоманенкоА. С.RomanenkoA. S.

Романенко Анатолий Сидорович, д.б.н., профессор, главный научный сотрудник

664033, г. Иркутск, ул. Лермонтова, 132, а/я 317

Anatoliy S. Romanenko Dr. Sci. (Chemistry), Professor, Chief Researcher

132, Lermontov St., Irkutsk, 664033

rom@sifibr.irk.ruСибирский институт физиологии и биохимии растений СО РАНРоссияSiberian Institute of Plant Physiology and Biochemistry SB RASRussian Federation202106102021113403412Copyright © Филинова Н.В., Ломоватская Л.А., Романенко А.С., 20212021Филинова Н.В., Ломоватская Л.А., Романенко А.С.Filinova N.V., Lomovatskaya L.A., Romanenko A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/648

Резюме: Целью данного исследования являлось изучение влияния различных концентраций ионов кальция на активность трансмембранной (тмАЦ) и растворимой форм аденилатциклаз (рАЦ) из клеток корней и стеблей растений двух сортов картофеля, контрастных по устойчивости к возбудителю кольцевой гнили Clavibacter michiganensis ssp. sepedonicus (Cms) на фоне воздействия его экзополисахаридов. Результаты экспериментов показали, что реакция тмАЦ из корней и стеблей на экзогенный Cа2+ оказалась почти противоположной у растений обоих сортов. В клетках корня растений устойчивого сорта 1 и 10 мМ Cа2+ весьма интенсивно активировал тмАЦ. В то же время в стебле средние концентрации Cа2+ ингибировали активность тмАЦ, а самые высокие – 1 и 10 мМ, на нее не влияли. Напротив, активность тмАЦ из клеток корня растений восприимчивого сорта незначительно активировалась повышенными концентрациями Cа2+, но в стеблях все концентрации Cа2+, начиная с 1 мкМ, существенно повышали активность тмАЦ. Таким образом, неодинаковая реакция аденилатциклаз растений картофеля обоих сортов на различные концентрации экзогенного кальция свидетельствует, скорее всего, о наличии нескольких изоформ этого фермента, отличающихся по чувствительности к ионам кальция. При этом, возможно, растения обоих сортов также отличаются по спектру таких изоформ. Исходя из того, что под воздействием экзополисахаридов Cms чувствительность к ионам кальция обеих форм аденилатциклаз существенно менялась в клетках растений обоих сортов, можно предположить, что эта особенность является одним из механизмов различной устойчивости растений данных сортов к патогену.

Abstract: This article aims to study the influence of different concentrations of calcium ions on the activity of transmembrane (tmAC) and soluble forms of adenylyl cyclase (sAC) in the cells of roots and stems of the plants of two types of potatoes. It compares and contrasts their stability to the agent of the annular rot Clavibacter michiganensis ssp. Sepedonicus (Cms) when exposed to its exopolysaccharides. The experimental results have shown that the reaction of tmAC from the roots and stems to exogenous Ca2+ was almost opposite in the plants of both types. In the root cells of the plants of the resistant types, 1 and 10 mM of Ca2+ have activated tmAC in a very intensive way. In the stem, the average concentrations of Ca2+ inhibited the tmAC activity, while the highest, 1 and 10 mM, did not affect it. the activity of tmAC taken from the root cells of the receptive type of plants was not activated significantly by the increased concentrations of Ca2+, whereas, in the stems, all the concentrations of Ca2+, tmAC activity increased substantially starting with 1 μM. Thus, the unequal reaction of adenylate cyclases of the potato plants of both types to different concentrations of exogenous calcium, testifies, most likely, the presence of several isoform of this ferment that differ in the sensitivity to calcium ions. At the same time, it is possible that the plants of both types may also differ in the spectrum of such isoforms. Since the influence of Cms exopolysaccharides significantly changes the sensitivity to the calcium ions of both forms of adenylate cyclases in the cells of plants of both types, it can be assumed that this feature is one of the mechanisms of these plants’ resistance to the pathogen.

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The authors declare that there are no conflicts of interest present.