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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vuzbiochemi</journal-id><journal-title-group><journal-title xml:lang="ru">Известия вузов. Прикладная химия и биотехнология</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of Universities. Applied Chemistry and Biotechnology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2227-2925</issn><issn pub-type="epub">2500-1558</issn><publisher><publisher-name>ИРНИТУ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21285/2227-2925-2021-11-3-413-420</article-id><article-id custom-type="elpub" pub-id-type="custom">vuzbiochemi-649</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PHYSICOCHEMICAL BIOLOGY</subject></subj-group></article-categories><title-group><article-title>Раствор азотной кислоты после обработки мискантуса как регулятор роста гороха посевного (Pisum sativum L.)</article-title><trans-title-group xml:lang="en"><trans-title>Nitric acid solution after treating miscanthus as a growth regulator of seed peas (Pisum sativum L.)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Скиба</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Skiba</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Скиба Екатерина Анатольевна, к.т.н., доцент, старший научный сотрудник</p><p>659322, г. Бийск, ул. Социалистическая</p></bio><bio xml:lang="en"><p>Ekaterina A. Skiba, Cand. Sci. (Engineering), Associate Professor, Senior Scientist</p><p>1, Socialisticheskaya St., Biysk, 659322</p></bio><email xlink:type="simple">eas08988@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Скиба</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Skiba</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Скиба Мария Алексеевна</p><p>659302, г. Бийск, ул. Михаила Кутузова, 9/3</p></bio><bio xml:lang="en"><p>Maria A. Skiba</p><p>9/3, Mikhail Kutuzov St., Biysk, 659302</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Пятунина</surname><given-names>О. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Pyatunina</surname><given-names>O. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Пятунина Ольга Ивановна, к.б.н., доцент, учитель биологии</p><p>659302, г. Бийск, ул. Михаила Кутузова, 9/3,</p></bio><bio xml:lang="en"><p>Olga I. Pyatunina, Cand. Sci. (Biology.), Associate Professor, Teacher</p><p>9/3, Mikhail Kutuzov St., Biysk, 659302</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт проблем химико-энергетических технологий СО РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Institute for Problems of Chemical and Energetic Technologies SB RAS</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Краевое государственное бюджетное общеобразовательное учреждение «Бийский лицей-интернат Алтайского края»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Biysk Lyceum School of Altai Region</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>06</day><month>10</month><year>2021</year></pub-date><volume>11</volume><issue>3</issue><fpage>413</fpage><lpage>420</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Скиба Е.А., Скиба М.А., Пятунина О.И., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Скиба Е.А., Скиба М.А., Пятунина О.И.</copyright-holder><copyright-holder xml:lang="en">Skiba E.A., Skiba M.A., Pyatunina O.I.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vuzbiochemi.elpub.ru/jour/article/view/649">https://vuzbiochemi.elpub.ru/jour/article/view/649</self-uri><abstract><p>Резюме: Мискантус во всем мире позиционируется как чрезвычайно перспективное быстровозобновляемое целлюлозосодержащее сырье для производства большого числа веществ химического и биотехнологического синтеза. В Институте проблем химико-энергетических технологий СО РАН разрабатываются авторские способы обработки мискантуса разбавленными растворами азотной кислоты, при этом количество отработанного раствора (жидкой фазы) в 20 раз больше, чем целевого продукта – твердой фазы, предназначенной для ферментативного гидролиза и дальнейшего микробиологического синтеза биоэтанола, бактериальной целлюлозы и других ценных продуктов. Была выдвинута гипотеза, что раствор азотной кислоты после обработки мискантуса, нейтрализованный гидратом аммония (далее препарат), представляет собой комбинированное лигногуминовое удобрение. Для проверки этой гипотезы исследована рострегулирующая активность препарата на примере семян гороха посевного. Установлено, что в зависимости от степени разведения и времени выдержки препарат действует двойственно: то как стимулятор, то как ингибитор роста. Так, при степени разведения 1:10 препарат действует как ингибитор, а при степени разведения 1:1 000 000 действие препарата перестает проявляться. Рабочим диапазоном является степень разведения от 1:100 до 1:10 000, когда наблюдается повышение энергии прорастания и всхожести на 2–6% по сравнению с контролем и стимулируется рост корней на 21–29%, то есть, проявляется ауксиноподобное ростстимулирующее действие. При длительной выдержке в течение 4-х суток препарат показывает ростингибирующее действие: снижаются энергия прорастания, всхожесть, длина стеблей и корней гороха посевного. Поскольку новый препарат в определенных условиях показал ростстимулирующую активность, можно считать подтвержденным, что он является комбинированным лигногуминовым удобрением.</p></abstract><trans-abstract xml:lang="en"><p>Abstract: All over the world, miscanthus is positioned as an extremely promising and rapidly renewable cellulose- containing raw material for the production of a large number of substances of chemical and biotechnological synthesis. The Institute for Problems of Chemical and Energetic Technologies of the Siberian Branch оf the Russian Academy of Sciences has been developing its own methods of treating miscanthus using diluted solutions of nitric acid. While the amount of a waste solution (liquid phase) is 20 times greater than the target product — a solid phase -- intended for enzymatic hydrolysis and further microbiological synthesis of bioethanol, bacterial cellulose and other valuable products. The hypothesis states that a nitric acid solution after treatment with miscanthus, which was neutralized with ammonium hydrate (hereinafter referred to as the preparation), is a combined lignohumic fertilizer. Testing this hypothesis has required studying the growth-regulating activity of the preparation using the example of sowing pea seeds. The results show that, depending on the degree of dilution and the exposure time, the preparation acts in two ways: either as a stimulant or as a growth inhibitor. Thus, at a dilution rate of 1:10, the preparation acts as an inhibitor, and at a dilution rate of 1:1,000,000, its effect ceases. The working range includes the dilution rate between 1:100 and 1:10,000, when an increase in germination energy and rate is observed by 2–6% compared to the control and root growth is stimulated by 21–29%, i.e. an auxin-like growth-stimulating effect is observed. With prolonged endurance during the 4th day, the preparation showed a growth-inhibiting effect, indicated by the decrease in the germination energy and rate, the length of the stems and roots of the sowing pea. The new preparation showing growth-stimulating activity under certain conditions, supposedly confirms the hypothesis that it is a combined lignohumic fertilizer.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>мискантус</kwd><kwd>азотная кислота</kwd><kwd>горох посевной</kwd><kwd>рострегулирование</kwd></kwd-group><kwd-group xml:lang="en"><kwd>miscanthus</kwd><kwd>nitric acid</kwd><kwd>seed peas</kwd><kwd>growth regulation</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена в рамках госзадания 121061500030-3.</funding-statement><funding-statement xml:lang="en">This work was performed under Governmental contract no. 121061500030-3.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Geissdoerfer M., Savaget P., Bocken N.M.P., Hultink E.J. 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