vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2022-12-3-417-423vuzbiochemi-846Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYРост и взаимодействие штаммов Trichoderma spp. при различных температурахTrichoderma spp. strains: growth and interaction at different temperatureshttps://orcid.org/0000-0002-2219-5801ПисаревскаяВ. А.PisarevskaiaV. A.

Виолетта Алексеевна Писаревская, студентка

125480, г. Москва, ул. Героев Панфиловцев, 20

Violetta A. Pisarevkaia, Student

20, Geroev Panfilovtsev St., 125480, Moscow

pisarevskayaviola@mail.ruhttps://orcid.org/0000-0002-4969-0765ЖуравлеваА. С.ZhuravliovaA. S.

Александра Сергеевна Журавлева, магистрант

125480, г. Москва, ул. Героев Панфиловцев, 20

Aleksandra S. Zhuravliova, Master Student

20, Geroev Panfilovtsev St., 125480, Moscow

Sashka.zhuravliova@yandex.ruhttps://orcid.org/0000-0002-8791-0271МиничМ. В.MinichM. V.

Мария Владимировна Минич, аспирант

125480, г. Москва, ул. Героев Панфиловцев, 20

Maria V. Minich, Postgraduate Student

20, Geroev Panfilovtsev St., 125480, Moscow

Mminich8@gmail.comhttps://orcid.org/0000-0002-0082-331XБехбудзадаН. Б.BehbudzadaN. B.

Нурлан Бешир-оглы Бехбудзада, аспирант

125480, г. Москва, ул. Героев Панфиловцев, 20

Nurlan B. Behbudzada, Postgraduate Student

20, Geroev Panfilovtsev St., 125480, Moscow

nnurlik@gmail.comhttps://orcid.org/0000-0002-8478-1160ШагаевА. А.ShagaevA. A.

Антон Александрович Шагаев, ассистент

125480, г. Москва, ул. Героев Панфиловцев, 20

Anton A. Shagaev, Assistant

20, Geroev Panfilovtsev St., 125480, Moscow,

shagaev.a.a@muctr.ruhttps://orcid.org/0000-0002-1595-2210МарквичевН. С.MarkvichevN. S.

Николай Семенович Марквичев, к.т.н., доцент

125480, г. Москва, ул. Героев Панфиловцев, 20

Nikolai S. Markvichev, Cand. Sci. (Engineering), Associate Professor

20, Geroev Panfilovtsev St., 125480, Moscow

markvichev.n.s@muctr.ruРоссийский химико-технологический университет им. Д. И. МенделееваРоссияMendeleev University of Chemical Technology of RussiaRussian Federation202207102022123417423Copyright © Писаревская В.А., Журавлева А.С., Минич М.В., Бехбудзада Н.Б., Шагаев А.А., Марквичев Н.С., 20222022Писаревская В.А., Журавлева А.С., Минич М.В., Бехбудзада Н.Б., Шагаев А.А., Марквичев Н.С.Pisarevskaia V.A., Zhuravliova A.S., Minich M.V., Behbudzada N.B., Shagaev A.A., Markvichev N.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/846

Данная работа посвящена изучению роста и взаимодействия штаммов Trichoderma spp. при различных температурах. Trichoderma spp. – это род микроскопических аскомицетов, грибы рода Trichoderma являются членами различных экосистем и обнаруживаются в почве, ризосфере. Цель исследования – изучить и сравнить характеристики роста и взаимодействия различных представителей грибов рода Trichoderma между собой. Для экспериментов использовали следующие среды: среду Чапека и среду, моделирующую экссудаты корневой системы растения (огурца обыкновенного). Была определена скорость роста штаммов Trichoderma spp. при различных температурах. Скорость роста на среде, содержащей модели экссудатов растения, отличалась от скорости роста на богатой питательной среде, также обнаружились различия в макроморфологии колоний. При температуре 9 °С на среде Чапека наблюдался рост штаммов Trichoderma longibrachiatum F2124, Trichoderma viride F2001; на среде, моделирующей экссудаты, – только штамма Trichoderma longibrachiatum F2124. При 40 °С отмечалось значительное торможение роста для всех штаммов, кроме представителей вида Trichoderma longibrachiatum. При температуре 50 °С роста колоний не наблюдалось. Все штаммы росли в температурном диапазоне от 12 до 28 °С. Было выдвинуто предположение о возможных различиях в характере взаимодействия представителей рода Trichoderma между собой при росте на различных средах. Исследовали взаимодействие между собой 3-х штаммов Trichoderma spp. при поверхностном культивировании в различных температурных условиях. По результатам исследования не обнаружилось значительных различий в характере взаимодействия представителей рода Trichoderma между собой.

The paper examines the growth of Trichoderma spp. strains and their interaction at different temperatures. Trichoderma spp. is a genus of microscopic ascomycetes; these fungi belong to different ecosystems and are found in soil (rhizosphere). The study aims to analyze and compare the characteristics pertaining to the growth of Trichoderma species and the interaction between them. The experiments used the following media: a Czapek medium and a medium simulating plant root exudates (Cucumis sativus). The growth rate of Trichoderma spp. strains was determined at different temperatures. The use of a medium containing plant exudate models provided a different growth rate as compared to that obtained when using a rich growth medium; some variations in the macromorphology of colonies were also observed. The growth of Trichoderma longibrachiatum F2124 and Trichoderma viride F2001 strains was detected at 9 °С on the Czapek medium, while only the Trichoderma longibrachiatum F2124 strain was observed to grow on the medium simulating exudates. A significant growth inhibition was noted at 40 °С for all strains, except for Trichoderma longibrachiatum species. No colony growth was observed at 50 °С. All the strains grew within the temperature range of 12 to 28 °С. It was assumed that Trichoderma species growing in various media may interact differently with each other. Interaction between the three strains of Trichoderma spp. was examined via the surface culture method under different temperature conditions. The study found no significant differences in the interaction between the Trichoderma species.

Trichodermaэкссудатытемператураскорость роставзаимодействиеTrichodermaexudatestemperaturegrowth rateinteractionReferencesKredics L., Hatvani L., Naeimi S., Körmöczi P., Manczinger L., Vágvölgyi C., et al. Biodiversity of the genus Hypocrea/Trichoderma in different habitats. In: Biotechnology and biology of Trichoderma. Elsevier; 2014, p. 3-24. https://doi.org/10.1016/B978-0-444-59576-8.00001-1.Kredics L., Hatvani L., Naeimi S., Körmöczi P., Manczinger L., Vágvölgyi C., et al. Biodiversity of the genus Hypocrea/Trichoderma in different habitats. In: Biotechnology and biology of Trichoderma. Elsevier; 2014, p. 3-24. https://doi.org/10.1016/B978-0-444-59576-8.00001-1.Kullnig C., Szakacs G., Kubicek C. P. Molecular identification of Trichoderma species from Russia, Siberia and the Himalaya. Mycological Research. 2000;104(9):1117-1125. https://doi.org/10.1017/S0953756200002604.Kullnig C., Szakacs G., Kubicek C. P. Molecular identification of Trichoderma species from Russia, Siberia and the Himalaya. Mycological Research. 2000;104(9):1117-1125. https://doi.org/10.1017/S0953756200002604.Poveda J., Eugui D., Abril-Urias P. Could Trichoderma be a plant pathogen? Successful root colonization. In: Trichoderma. Rhizosphere biology. Sharma A., Sharma P. (eds.). Springer, Singapore; 2020, p. 35-59. http://dx.doi.org/10.1007/978-981-15-3321-1_3.Poveda J., Eugui D., Abril-Urias P. Could Trichoderma be a plant pathogen? Successful root colonization. In: Trichoderma. Rhizosphere biology. Sharma A., Sharma P. (eds.). Springer, Singapore; 2020, p. 35-59. http://dx.doi.org/10.1007/978-981-15-3321-1_3.Rubini M. R., Silva-Ribeiro R. T., Pomella A. W. V., Maki C. S., Araújo W. L., dos Santos D. R., et al. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches’ Broom Disease. International Journal of Biological Sciences. 2005;1(1):24-33. https://dx.doi.org/10.7150%2Fijbs.1.24.Rubini M. R., Silva-Ribeiro R. T., Pomella A. W. V., Maki C. S., Araújo W. L., dos Santos D. R., et al. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches’ Broom Disease. International Journal of Biological Sciences. 2005;1(1):24-33. https://dx.doi.org/10.7150%2Fijbs.1.24.Kredics L., Jimenez G. L., Naeimi S., Czifra D., Urbán P., Manczinger L., et al. A challenge to mushroom growers: the green mould disease of cultivated champignons. In: Current research, technology and education topics in applied microbiology and microbial biotechnology. Méndez-Vilas A. (ed.). FORMATEX; 2010, p. 295-305.Kredics L., Jimenez G. L., Naeimi S., Czifra D., Urbán P., Manczinger L., et al. A challenge to mushroom growers: the green mould disease of cultivated champignons. In: Current research, technology and education topics in applied microbiology and microbial biotechnology. Méndez-Vilas A. (ed.). FORMATEX; 2010, p. 295-305.Höller U., Wright A. D., Matthee G. F., Konig G. M., Draeger S., Aust H.-J., et al. Fungi from marine sponges: diversity, biological activity and secondary metabolites. Mycological Research. 2000;104(11):1354-1365. http://dx.doi.org/10.1017/S0953756200003117.Höller U., Wright A. D., Matthee G. F., Konig G. M., Draeger S., Aust H.-J., et al. Fungi from marine sponges: diversity, biological activity and secondary metabolites. Mycological Research. 2000;104(11):1354-1365. http://dx.doi.org/10.1017/S0953756200003117.Zin N. A., Badaluddin N. A. Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences. 2020;65(2):168-178. https://doi.org/10.1016/j.aoas.2020.09.003.Zin N. A., Badaluddin N. A. Biological functions of Trichoderma spp. for agriculture applications. Annals of Agricultural Sciences. 2020;65(2):168-178. https://doi.org/10.1016/j.aoas.2020.09.003.Monte E. Understanding Trichoderma: between biotechnology and microbial ecology. International Journal of Microbiology. 2001;4(1):1-4. https://doi.org/10.1007/s101230100001.Monte E. Understanding Trichoderma: between biotechnology and microbial ecology. International Journal of Microbiology. 2001;4(1):1-4. https://doi.org/10.1007/s101230100001.Sani M. N. H., Hasan M., Uddain J., Subramaniam S. Impact of application of Trichoderma and biochar on growth, productivity and nutritional quality of tomato under reduced N-P-K fertilization. Annals of Agricultural Sciences. 2020;65(1):107-115. https://doi.org/10.1016/j.aoas.2020.06.003.Sani M. N. H., Hasan M., Uddain J., Subramaniam S. Impact of application of Trichoderma and biochar on growth, productivity and nutritional quality of tomato under reduced N-P-K fertilization. Annals of Agricultural Sciences. 2020;65(1):107-115. https://doi.org/10.1016/j.aoas.2020.06.003.Lucini L., Colla G., Moreno M. B. M., Bernardo L., Cardarelli M., Terzi V., et al. Inoculation of Rhizoglomus irregulare or Trichoderma atroviride differentially modulates metabolite profiling of wheat root exudates. Phytochemistry. 2019;157:158-167. https://doi.org/10.1016/j.phytochem.2018.10.033.Lucini L., Colla G., Moreno M. B. M., Bernardo L., Cardarelli M., Terzi V., et al. Inoculation of Rhizoglomus irregulare or Trichoderma atroviride differentially modulates metabolite profiling of wheat root exudates. Phytochemistry. 2019;157:158-167. https://doi.org/10.1016/j.phytochem.2018.10.033.Caporale A. G., Sommella A., Lorito M., Lombardi N., Azam S. M. G. G., Pigna M., et al. Trichoderma spp. alleviate phytotoxicity in lettuce plants (Lactuca sativa L.) irrigated with arsenic-contaminated water. Journal of Plant Physiology. 2014;171(15):1378-1384. https://doi.org/10.1016/j.jplph.2014.05.011.Caporale A. G., Sommella A., Lorito M., Lombardi N., Azam S. M. G. G., Pigna M., et al. Trichoderma spp. alleviate phytotoxicity in lettuce plants (Lactuca sativa L.) irrigated with arsenic-contaminated water. Journal of Plant Physiology. 2014;171(15):1378-1384. https://doi.org/10.1016/j.jplph.2014.05.011.Vargas J. T., Rodríguez-Monroy M., Meyer M. L., Montes-Belmont R., Sepúlveda-Jiménez G. Trichoderma asperellum ameliorates phytotoxic effects of copper in onion (Allium cepa L.). Environmental and Experimental Botany. 2017;136:85-93. https://doi.org/10.1016/j.envexpbot.2017.01.009.Vargas J. T., Rodríguez-Monroy M., Meyer M. L., Montes-Belmont R., Sepúlveda-Jiménez G. Trichoderma asperellum ameliorates phytotoxic effects of copper in onion (Allium cepa L.). Environmental and Experimental Botany. 2017;136:85-93. https://doi.org/10.1016/j.envexpbot.2017.01.009.López-Mondéjar R., Ros M., Pascual J. A. Mycoparasitism-related genes expression of Trichoderma harzianum isolates to evaluate their efficacy as biological control agent. Biological Control. 2011;56(1):59-66. https://doi.org/10.1016/j.biocontrol.2010.10.003.López-Mondéjar R., Ros M., Pascual J. A. Mycoparasitism-related genes expression of Trichoderma harzianum isolates to evaluate their efficacy as biological control agent. Biological Control. 2011;56(1):59-66. https://doi.org/10.1016/j.biocontrol.2010.10.003.Rajani P., Rajasekaran C., Vasanthakumari M. M., Olsson S. B., Ravikanth G., Shaanker R. U. Inhibition of plant pathogenic fungi by endophytic Trichoderma spp. through mycoparasitism and volatile organic compounds. Microbiological Research. 2021;242:126595. https://doi.org/10.1016/j.micres.2020.126595.Rajani P., Rajasekaran C., Vasanthakumari M. M., Olsson S. B., Ravikanth G., Shaanker R. U. Inhibition of plant pathogenic fungi by endophytic Trichoderma spp. through mycoparasitism and volatile organic compounds. Microbiological Research. 2021;242:126595. https://doi.org/10.1016/j.micres.2020.126595.Metz N., Hausladen H. Trichoderma spp. as potential biological control agent against Alternaria solani in potato. Biological Control. 2022;166:104820. https://doi.org/10.1016/j.biocontrol.2021.104820.Metz N., Hausladen H. Trichoderma spp. as potential biological control agent against Alternaria solani in potato. Biological Control. 2022;166:104820. https://doi.org/10.1016/j.biocontrol.2021.104820.Jambhulkar P. P., Raja M., Singh B., Katoch S., Kumar S., Sharma P. Potential native Trichoderma strains against Fusarium verticillioides causing post flowering stalk rot in winter maize. Crop Protection. 2021;152:105838. https://doi.org/10.1016/j.cropro.2021.105838.Jambhulkar P. P., Raja M., Singh B., Katoch S., Kumar S., Sharma P. Potential native Trichoderma strains against Fusarium verticillioides causing post flowering stalk rot in winter maize. Crop Protection. 2021;152:105838. https://doi.org/10.1016/j.cropro.2021.105838.Carvalho D. D. C., Junior M. L., Martins I., Inglis P. W., Mello S. C. M. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology. 2014;39(5):384-391. https://doi.org/10.1590/S1982-56762014000500005.Carvalho D. D. C., Junior M. L., Martins I., Inglis P. W., Mello S. C. M. Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology. 2014;39(5):384-391. https://doi.org/10.1590/S1982-56762014000500005.Cherif M., Benhamou N. Cytochemical aspects of chitin breakdown during the parasitic action of a Trichoderma sp. on Fusarium oxysporum f. sp. radicis-lycopersici. Phytopathology. 1990;80(12):1406-1414. https://doi.org/10.1094/PHYTO-80-1406.Cherif M., Benhamou N. Cytochemical aspects of chitin breakdown during the parasitic action of a Trichoderma sp. on Fusarium oxysporum f. sp. radicis-lycopersici. Phytopathology. 1990;80(12):1406-1414. https://doi.org/10.1094/PHYTO-80-1406.Shakeri J., Foster H. A. Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects. Enzyme and Microbial Technology. 2007;40(4):961-968. http://dx.doi.org/10.1016/j.enzmictec.2006.07.041.Shakeri J., Foster H. A. Proteolytic activity and antibiotic production by Trichoderma harzianum in relation to pathogenicity to insects. Enzyme and Microbial Technology. 2007;40(4):961-968. http://dx.doi.org/10.1016/j.enzmictec.2006.07.041.Juan Z., Ting L., Wei-Cheng L., Dian-Peng Z., Dan D., Hui-Ling W., et al. Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants. Journal of Integrative Agriculture. 2021;20(5):1266-1276. https://doi.org/10.1016/S2095-3119(20)63415-3.Juan Z., Ting L., Wei-Cheng L., Dian-Peng Z., Dan D., Hui-Ling W., et al. Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants. Journal of Integrative Agriculture. 2021;20(5):1266-1276. https://doi.org/10.1016/S2095-3119(20)63415-3.da Silva L. R., Valadares-Inglis M. C., Peixoto G. H. S., de Luccas B. E. G., Costa Muniz P. H. P., Magalhães D. M., et al. Volatile organic compounds emitted by Trichoderma azevedoi promote the growth of lettuce plants and delay the symptoms of white mold. Biological Control. 2021;152:104447. https://doi.org/10.1016/j.biocontrol.2020.104447.da Silva L. R., Valadares-Inglis M. C., Peixoto G. H. S., de Luccas B. E. G., Costa Muniz P. H. P., Magalhães D. M., et al. Volatile organic compounds emitted by Trichoderma azevedoi promote the growth of lettuce plants and delay the symptoms of white mold. Biological Control. 2021;152:104447. https://doi.org/10.1016/j.biocontrol.2020.104447.Rosyida V. T., Indrianingsih A. W., Maryana R., Wahono S. K. Effect of temperature and fermentation time of crude cellulase production by Trichoderma Reesei on straw substrate. Energy Procedia. 2015;65:368-371. https://doi.org/10.1016/j.egypro.2015.01.065.Rosyida V. T., Indrianingsih A. W., Maryana R., Wahono S. K. Effect of temperature and fermentation time of crude cellulase production by Trichoderma Reesei on straw substrate. Energy Procedia. 2015;65:368-371. https://doi.org/10.1016/j.egypro.2015.01.065.

The authors declare that there are no conflicts of interest present.