vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2022-12-3-438-446vuzbiochemi-848Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYОценка биобезопасности наноструктурных минералов, разработанных для применения в качестве удобренийNanostructured minerals developed to be used as fertilizers: biosafety evaluationhttps://orcid.org/0000-0002-1575-8493ДегтяреваИ. А.DegtyarevaI. A.

Ирина Александровна Дегтярева, д.б.н., главный научный сотрудник

420059, г. Казань, ул. Оренбургский тракт, 20А

Irina A. Degtyareva, Dr. Sci. (Biology), Chief Researcher

20A, Orenburg tract St., 420059, Kazan

peace-1963@mail.ruhttps://orcid.org/0000-0003-2285-8879БабынинЭ. В.BabyninE. V.

Эдуард Викторович Бабынин, к.б.н., старший научный сотрудник

420059, г. Казань, ул. Оренбургский тракт, 20А

Eduard V. Babynin, Cand. Sci. (Biology), Senior Researcher

20A, Orenburg tract St., 420059, Kazan

edward.b67@mail.ruhttps://orcid.org/0000-0002-9068-3014ПрищепенкоЕ. А.PrishchepenkoE. A.

Елена Александровна Прищепенко, к.с.-х.н., руководитель

420059, г. Казань, ул. Оренбургский тракт, 20А

Elena A. Prishchepenko, Cand. Sci. (Agriculture), Director

20A, Orenburg tract St., 420059, Kazan

pea77@list.ruТатарский научно-исследовательский институт агрохимии и почвоведения – обособленное структурное подразделение Федерального государственного бюджетного учреждения науки «Федеральный исследовательский центр «Казанский научный центр Российской академии наук»РоссияScientific Institution Tatar Scientific Research Institute of Agricultural Chemistry and Soil Science – Subdivision of the Federal State Budgetary Institution of Science «Kazan Scientific Center of the Russian Academy of Sciences»Russian Federation202207102022123438446Copyright © Дегтярева И.А., Бабынин Э.В., Прищепенко Е.А., 20222022Дегтярева И.А., Бабынин Э.В., Прищепенко Е.А.Degtyareva I.A., Babynin E.V., Prishchepenko E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/848

Природные цеолиты эффективно используются в качестве удобрений, субстратов и носителей для пестицидов, а также сорбентов при восстановлении загрязненных почв. Поскольку наноструктурные минералы обладают уникальными физико-химическими свойствами, прежде чем использовать их на практике, они должны быть проверены на наличие токсичности и генотоксичности. Впервые нами проведена проверка мутагенных и антимутагенных свойств наноструктурной водно-цеолитной суспензии с использованием 2-х бактериальных тест-систем: теста Эймса и SOS-lux теста. Согласно полученным данным, наноструктурная водно-цеолитная суспензия не обладает мутагенной активностью в исследованном диапазоне концентраций (0,75–400 мкг/мл). Для оценки антимутагенной активности наноструктурной водно-цеолитной суспензии выбраны различные типы мутагенов: митомицин С, этилметансульфонат, 2,4-динитрофенилгидразин, а также ДНК-повреждающие агенты – офлоксацин и перекись водорода. Показан значительный антимутагенный эффект наноструктурной водно-цеолитной суспензии в концентрации 200 мкг/мл в отношении митомицина С в SOS-lux тесте (ингибирование мутагенной активности составило 50,0%) и 2,4-динитрофенилгидразина в тесте Эймса (ингибирование – 62,0%). Для остальных мутагенов отмечен слабый антимутагенный эффект (17,0% для этилметансульфоната), а в отношении офлоксацина и перекиси водорода антимутагенный эффект отсутствует. Различия в антимутагенном эффекте можно объяснить тем, что цеолиты отрицательно заряжены, поэтому могут захватывать только положительные (или нейтральные) молекулы. Следовательно, антимутагенный эффект наноструктурной водно-цеолитной суспензии будет зависеть от заряда молекулы мутагена. На основании полученных результатов можно считать наноструктурную водно-цеолитную суспензию безопасной для окружающей среды, что позволяет использовать ее по агропромышленному назначению в качестве удобрения при выращивании сельскохозяйственных культур.

Natural zeolites are effectively used as fertilizers, substrates, and pesticide carriers, as well as sorbents in the remediation of contaminated soils. Since nanostructured minerals exhibit unique physicochemical properties, they must be tested for toxicity and genotoxicity prior to their use in practice. The mutagenic and antimutagenic properties of a nanostructured water-zeolite suspension were first tested using two bacterial test systems: Ames test and SOS-lux test. According to the obtained data, the nanostructured water-zeolite suspension exhibits no mutagenic activity within the analyzed concentration range (0.75–400 µg/mL). In order to assess the antimutagenic activity of the nanostructured water-zeolite suspension, different types of mutagens were selected: mitomycin C, ethyl methanesulfonate, 2,4-dinitrophenylhydrazine, as well as DNA-damaging agents (ofloxacin and hydrogen peroxide). A significant antimutagenic effect of the nanostructured water-zeolite suspension at 200 μg/mL was shown against mitomycin C in the SOS-lux test (50.0% inhibition of mutagenic activity) and 2,4-dinitrophenylhydrazine in the Ames test (62.0% inhibition). For the other mutagens, a weak antimutagenic effect was observed (17.0% for ethyl methanesulfonate), while no antimutagenic effect was reported for ofloxacin and hydrogen peroxide. These differences can be attributed to the negative charge in zeolites, meaning that they can capture only positive (or neutral) molecules. Therefore, the antimutagenic effect of the nanostructured water-zeolite suspension depends on the charge of the mutagen molecule. According to the obtained results, the nanostructured water-zeolite suspension can be considered environmentally friendly, which allows it to be used for agro-industrial purposes as a fertilizer in the production of crops.

биобезопасностьминералынаноструктурная водно-цеолитная суспензиямутагенные и антимутагенные свойстваnanostructured water-zeolite suspensionbiosafetymineralsnanostructured water-zeolite suspensionmutagenic propertiesantimutagenic propertiesРабота выполнена в рамках государственного задания №FMEG-2021-0003, регистрационный номер 121021600147-1.The work was carried out within the framework of the state task no. FMEG-2021-0003, registration number 121021600147-1.ReferencesKhan M. N., Mobin M., Abbas Z. K., AlMutairi K. A., Siddiqui Z. H. Role of nanomaterials in plants under challenging environments // Plant Physiology and Biochemistry. 2017. Vol. 110. P. 194-209. https://doi.org/10.1016/j.plaphy.2016.05.038.Khan M. N., Mobin M., Abbas Z. K., AlMutairi K. A., Siddiqui Z. H. Role of nanomaterials in plants under challenging environments. Plant Physiology and Biochemistry. 2017;110:194-209. https://doi.org/10.1016/j.plaphy.2016.05.038.Usman M., Farooq M., Wakeel A., Nawaz A., Cheema S. A., Rehman H. U., et al. Nanotechnology in agriculture: current status, challenges and future opportunities // Science of the Total Environment. 2020. Vol. 721. P. 137778. https://doi.org/10.1016/j.scitotenv.2020.137778.Usman M., Farooq M., Wakeel A., Nawaz A., Cheema S. A., Rehman H. U., et al. Nanotechnology in agriculture: current status, challenges and future opportunities. Science of the Total Environment. 2020;721:137778. https://doi.org/10.1016/j.scitotenv.2020.137778.Sanzari I., Leone A., Ambrosone A. Nanotechnology in plant science: to make a long story short // Frontiers in Bioengineering and Biotechnology. 2019. Vol. 7. https://doi.org/10.3389/fbioe.2019.00120.Sanzari I., Leone A., Ambrosone A. Nanotechnology in plant science: to make a long story short. Frontiers in Bioengineering and Biotechnology. 2019;7. https://doi.org/10.3389/fbioe.2019.00120.van Dijk M., Meijerink G. W. A review of food security scenario studies: gaps and ways forward. In: The food puzzle: pathways to securing food for all. Wageningen UR, 2014. P. 30-32.van Dijk M., Meijerink G. W. A review of food security scenario studies: gaps and ways forward. In: The food puzzle: pathways to securing food for all. Wageningen UR; 2014, p. 30-32.Manjaiah K. M., Mukhopadhyay R., Paul R., Datta S. C., Kumararaja P., Sarkar B. Clay minerals and zeolites for environmentally sustainable agriculture. In: Modified clay and zeolite nanocomposite materials. The Netherlands, 2019. P. 309-329. https://doi.org/10.1016/B978-0-12-814617-0.00008-6.Manjaiah K. M., Mukhopadhyay R., Paul R., Datta S. C., Kumararaja P., Sarkar B. Clay minerals and zeolites for environmentally sustainable agriculture. In: Modified clay and zeolite nanocomposite materials. The Netherlands; 2019, p. 309-329. https://doi.org/10.1016/B978-0-12-814617-0.00008-6.Mumpton F. A. La roca magica: uses of natural zeolites in agriculture and industry // Proceedings of the National Academy of Sciences. 1999. Vol. 96, no. 7. P. 3463-3470. https://doi.org/10.1073/pnas.96.7.3463.Mumpton F. A. La roca magica: uses of natural zeolites in agriculture and industry. Proceedings of the National Academy of Sciences. 1999;96(7):3463-3470. https://doi.org/10.1073/pnas.96.7.3463.Oliveira C. R., Rubio J. Adsorption of ions onto treated natural zeolite // Materials Research. 2007. Vol. 10, no. 4. https://doi.org/10.1590/S1516-14392007000400014.Oliveira C. R., Rubio J. Adsorption of ions onto treated natural zeolite. Materials Research. 2007;10(4). https://doi.org/10.1590/S1516-14392007000400014.Chinnamuthu C. R., Boopathi P. M. Nanotechnology and agroecosystem // Madras Agricultural Journal. 2009. Vol. 96, no. 1-6. P. 17-31.Chinnamuthu C. R., Boopathi P. M. Nanotechnology and agroecosystem. Madras Agricultural Journal. 2009;96(1-6):17-31.Arora S., Rajwade J. M., Paknikar K. M. Nanotoxicology and in vitro studies: the need of the hour // Toxicology and Applied Pharmacology. 2012. Vol. 258, no. 2. P. 151-165. https://doi.org/10.1016/j.taap.2011.11.010.Arora S., Rajwade J. M., Paknikar K. M. Nanotoxicology and in vitro studies: the need of the hour. Toxicology and Applied Pharmacology. 2012;258(2):151-165. https://doi.org/10.1016/j.taap.2011.11.010.Lin D., Xing B. Root uptake and phytotoxicity of ZnO nanoparticles // Environmental Science and Technology. 2008. Vol. 42, no. 15. P. 5580-5585. https://doi.org/10.1021/es800422x.Lin D., Xing B. Root uptake and phytotoxicity of ZnO nanoparticles. Environmental Science and Technology. 2008;42(15):5580-5585. https://doi.org/10.1021/es800422x.Petushkov A., Ndiege N., Salem A. K., Larsen S. C. Toxicity of silica nanomaterials: zeolites, mesoporous silica, and amorphous silica nanoparticles // Advances in Molecular Toxicology. 2010. Vol. 4. P. 223-266. https://doi.org/10.1016/S1872-0854(10)04007-5.Petushkov A., Ndiege N., Salem A. K., Larsen S. C. Toxicity of silica nanomaterials: zeolites, mesoporous silica, and amorphous silica nanoparticles. Advances in Molecular Toxicology. 2010;4:223-266. https://doi.org/10.1016/S1872-0854(10)04007-5.Tarafdar J. C., Xiang Y., Wang W.-N., Dong Q., Biswas P. Standardization of size, shape and concentration of nanoparticle for plant application // Applied Biological Research. 2012. Vol. 14, no. 2. P. 138-144.Tarafdar J. C., Xiang Y., Wang W.-N., Dong Q., Biswas P. Standardization of size, shape and concentration of nanoparticle for plant application. Applied Biological Research. 2012;14(2):138-144.Дегтярева И. А., Бабынин Э. В., Мотина Т. Ю., Давлетшина А. Я., Яппаров И. А. Оценка мутагенных и антимутагенных свойств наноструктурного фосфорита - компонента комплексного удобрения // Агрохимический вестник. 2019. N 1. С. 41-45. https://doi.org/10.24411/0235-2516-2019-10010.Degtyareva I. A., Babynin E. V., Motina T. Yu., Davletshina A. Ya., Yapparov I. A. Estimation of mutagenic and antimutagenic properties of nanostructured phosphorus - component of complex fertilizer. Agrokhimicheskii vestnik = Agrochemical Herald. 2019;(1):41-45. (In Russian). https://doi.org/10.24411/0235-2516-2019-10010.Degtyareva I. A., Ezhkova A. M., Yapparov A. Kh., Yapparov I. A., Ezhkov V. O., Babynin E. V., et al. Production of nano-bentonite and the study of its effect on mutagenesis in bacteria Salmonella typhimurium // Nanotechnologies in Russia. 2016. Vol. 11, no. 9-10. P. 663-670. https://doi.org/10.1134/S1995078016050050.Degtyareva I. A., Ezhkova A. M., Yapparov A. Kh., Yapparov I. A., Ezhkov V. O., Babynin E. V., et al. Production of nano-bentonite and the study of its effect on mutagenesis in bacteria Salmonella typhimurium. Nanotechnologies in Russia. 2016;11(9-10):663-670. https://doi.org/10.1134/S1995078016050050.Дурнев А. Д., Суслова Т. Б., Черемисина З. П., Дубовская О. Ю., Нигарова Э. А., Коркина Л. Г. [и др.]. Исследование мутагенного действия пыли природных цеолитов и хризотил-асбеста // Экспериментальная онкология. 1990. Т. 12. N 2. С. 21-24.Durnev A. D., Suslova T. B., Cheremisina Z. P., Dubovskaya O. Yu., Nigarova E. A., Korkina L. G., et al. Study of the mutagenic effect of natural zeolite dust and chrysotile asbestos. Eksperimental’naya onkologiya. 1990;12(2):21-24. (In Russian).Ilgren E. B., Brena M. O., Larragoitia J. C., Navarrete G. L., Berna A. F., Krauss E., et al. A reconnaissance study of a potential emerging Mexican mesothelioma epidemic due to fibrous zeolite exposure // Indoor and Built Environment. 2008. Vol. 17, no. 6. P. 496-515. https://doi.org/10.1177/1420326X08096610.Ilgren E. B., Brena M. O., Larragoitia J. C., Navarrete G. L., Berna A. F., Krauss E., et al. A reconnaissance study of a potential emerging Mexican mesothelioma epidemic due to fibrous zeolite exposure. Indoor and Built Environment. 2008;17(6):496-515. https://doi.org/10.1177/1420326X08096610.Pavelic K., Hadzija M. Medical applications of zeolites. In: Handbook of zeolite science and technology. New York, 2003. P. 1143-1174.Pavelic K., Hadzija M. Medical applications of zeolites. In: Handbook of zeolite science and technology. New York; 2003, p. 1143-1174.Bunn W. B., Bender J. R., Hesterberg T. W., Chase G. R., Konzen J. L. Recent studies of manmade vitreous fibers. Chronic animal inhalation studies // Journal of Occupational Medicine. 1993. Vol. 35, no. 2. P. 101-113. https://doi.org/10.1097/00043764-199302000-00009.Bunn W. B., Bender J. R., Hesterberg T. W., Chase G. R., Konzen J. L. Recent studies of man-made vitreous fibers. Chronic animal inhalation studies. Journal of Occupational Medicine. 1993;35(2):101-113. https://doi.org/10.1097/00043764-199302000-00009.Maron D. M., Ames B. N. Revised methods for the Salmonella mutagenicity test // Mutation Research. 1983. Vol. 113, no. 3-4. P. 173-215. https://doi.org/10.1016/0165-1161(83)90010-9.Maron D. M., Ames B. N. Revised methods for the Salmonella mutagenicity test. Mutation Research. 1983;113(3-4):173-215. https://doi.org/10.1016/0165-1161(83)90010-9.Evandri M. G., Battinelli L., Daniele C., Mastrangelo S., Bolle P., Mazzanti G. The antimutagenic activity of Lavandula angustifolia (lavender) essential oil in the bacterial reverse mutation assay // Food and Chemical Toxicology. 2005. Vol. 43, no. 9. P. 1381-1387. https://doi.org/10.1016/j.fct.2005.03.013.Evandri M. G., Battinelli L., Daniele C., Mastrangelo S., Bolle P., Mazzanti G. The antimutagenic activity of Lavandula angustifolia (lavender) essential oil in the bacterial reverse mutation assay. Food and Chemical Toxicology. 2005;43(9):1381-1387. https://doi.org/10.1016/j.fct.2005.03.013.Cooper D. L., Lovett S. T. Toxicity and tolerance mechanisms for azidothymidine, a replication gap-promoting agent, in Escherichia coli // DNA Repair (Amst). 2011. Vol. 10, no. 3. P. 260-270.Cooper D. L., Lovett S. T. Toxicity and tolerance mechanisms for azidothymidine, a replication gappromoting agent, in Escherichia coli. DNA Repair (Amst). 2011;10(3):260-270.Caldini G., Trotta F., Villarini M., Moretti M., Pasquini R., Scassellati-Sforzolini G., et al. Screening of potential lactobacilli antigenotoxicity by microbial and mammalian cell-based tests // International Journal of Food Microbiology. 2005. Vol. 102, no. 1. P. 37-47. https://doi.org/10.1016/j.ijfoodmi-cro.2004.11.015.Caldini G., Trotta F., Villarini M., Moretti M., Pasquini R., Scassellati-Sforzolini G., et al. Screening of potential lactobacilli antigenotoxicity by microbial and mammalian cell-based tests. International Journal of Food Microbiology. 2005;102(1):37-47. https://doi.org/10.1016/j.ijfoodmicro.2004.11.015.Tagu D., Le Trionnaire G., Tanguy S., Gauthier J.-P., Huynh J.-R. EMS mutagenesis in the pea aphid Acyrthosiphon pisum // G3 Genes, Genomes, Genetics. 2014. Vol. 4, no. 4. P. 657-667. https://doi.org/10.1534/g3.113.009639.Tagu D., Le Trionnaire G., Tanguy S., Gauthier J.-P., Huynh J.-R. EMS mutagenesis in the pea aphid Acyrthosiphon pisum. G3 Genes, Genomes, Genetics. 2014;4(4):657-667. https://doi.org/10.1534/g3.113.009639.Abraham L. M., Selva D., Casson R., Leibovitch I. Mitomycin: clinical applications in ophthalmic practice // Drugs. 2006. Vol. 66, no. 3. P. 321-340. https://doi.org/10.2165/00003495-200666030-00005.Abraham L. M., Selva D., Casson R., Leibovitch I. Mitomycin: clinical applications in ophthalmic practice. Drugs. 2006;66(3):321-340. https://doi.org/10.2165/00003495-200666030-00005.Valverde M., Lozano-Salgado J., Fortini P., Rodriguez-Sastre M. A., Rojas E., Dogliotti E. Hydrogen peroxide-induced DNA damage and repair through the differentiation of human adipose-derived mesenchymal stem cells // Stem Cells International. 2018. P. 1615497. https://doi.org/10.1155/2018/1615497.Valverde M., Lozano-Salgado J., Fortini P., Rodriguez-Sastre M. A., Rojas E., Dogliotti E. Hydrogen peroxide-induced DNA damage and repair through the differentiation of human adipose-derived mesenchymal stem cells. Stem Cells International. 2018:1615497. https://doi.org/10.1155/2018/1615497.Parodi S., De Flora S., Cavanna M., Pino A., Robbiano L., Bennicelli C., et al. DNA-damaging activity in vivo and bacterial mutagenicity of sixteen hydrazine derivatives as related quantitatively to their carcinogenicity // Cancer Research. 1981. Vol. 41, no. 4. P. 1469-1482.Parodi S., De Flora S., Cavanna M., Pino A., Robbiano L., Bennicelli C., et al. DNA-damaging activity in vivo and bacterial mutagenicity of sixteen hydrazine derivatives as related quantitatively to their carcinogenicity. Cancer Research. 1981;41(4):1469-1482.Amarh V., Arthur P. K. DNA double-strand break formation and repair as targets for novel antibiotic combination chemotherapy // Future Science OA. 2019. Vol. 5, no. 8. P. FSO411. https://doi.org/10.2144/fsoa-2019-0034.Amarh V., Arthur P. K. DNA double-strand break formation and repair as targets for novel antibiotic combination chemotherapy. Future Science OA. 2019;5(8):FSO411. https://doi.org/10.2144/fsoa-2019-0034.

The authors declare that there are no conflicts of interest present.