vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2022-12-4-557-565vuzbiochemi-898Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYВлияние штаммов Bacillus thuringiensis на ростовые и метаболические процессы в проростках Pisum sativum L.Effect of Bacillus thuringiensis strains on growth and metabolic processes in Pisum sativum L. sproutshttps://orcid.org/0000-0001-5401-0579КрыжкоА. В.KryzhkoA. V.

Анастасия Владимировна Крыжко - кандидат сельскохозяйственных наук, ведущий научный сотрудник лаборатории молекулярной генетики, протеомики и биоинформатики в сельском хозяйстве.

295453, Симферополь, ул. Киевская, 150

Anastasiia V. Kryzhko - Cand. Sci. (Agriculture), Leading Researcher, Laboratory of Molecular Genetics, Proteomics and Bioinformatics in Agriculture.

150, Kievskaya St., Simferopol, 295453

kryzhko_a@niishk.ruhttps://orcid.org/0000-0002-5931-0412СмаглийН. Н.SmagliyN. N.

Наталья Николаевна Смаглий - лаборант лаборатории молекулярной и клеточной биологии.

295453, Симферополь, ул. Киевская, 150

Natalia N. Smagliy - Assistant, Laboratory of Molecular and Cellular Biology/

150, Kievskaya St., Simferopol, 295453

scarletsun7991@mail.ruНаучно-исследовательский институт сельского хозяйства КрымаРоссияInstitute of Agriculture of CrimeaRussian Federation202201012023124557565Copyright © Крыжко А.В., Смаглий Н.Н., 20232023Крыжко А.В., Смаглий Н.Н.Kryzhko A.V., Smagliy N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/898

Энтомопатогенные бактерии Bacillus thuringiensis Berliner являются широко известным биоагентом препаратов для регуляции численности листогрызущих насекомых-вредителей сельскохозяйственных и декоративных культур. Помимо энтомопатогенных свойств, перспективно изучение и других агрономически полезных свойств данного микроорганизма. Целью наших исследований было рассмотрение ростстимулирующего эффекта энтомопатогенных штаммов B. thuringiensis на растения гороха сорта Девиз. Материалом для исследований послужили энтомопатогенные штаммы B. thuringiensis 685, 926 и 109-С, полученные из Крымской коллекции микроорганизмов Научноисследовательского института сельского хозяйства Крыма, зарегистрированной на сайте (http://www.ckp-rf.ru) под номером 507484. Морфометрические параметры проростков гороха оценивали согласно стандартным методикам. Биохимические показатели проростков гороха определяли в 10-суточных проростках. Активность амилазы проростков гороха определяли фотокалориметрически, общую кислотность – титрованием с 0,1 н раствора NaOH, суммарное содержание водорастворимых фенольных соединений – титриметрическим методом Левенталя. Установлено, что жидкая споровая культура штаммов B. thuringiensis 685, 926 и 109-С оказывала стимулирующее воздействие на длину корня и стебля проростка, на вес 10-суточных проростков гороха сорта Девиз. Обработка споровой суспензией всех исследованных штаммов B. thuringiensis способствовала увеличению содержания органических кислот в проростках в среднем на 12,4% в сравнении с контролем. Максимальное стимулирующее действие на амилолитическую активность и синтез фенольных соединений в проростках гороха сорта Девиз оказывала обработка споровой суспензией штамма B. thuringiensis 926. Активность амилаз увеличивалась в среднем на 41,5% к контролю, а суммарное содержание фенольных соединений в данном варианте было в 2,3 раза выше, чем в контроле. Таким образом, принимая во внимание энтомопатогенные свойства и полученные данные о ростстимулирующей активности штаммов B. thuringiensis можно заключить, что данные бактерии имеют дополнительный потенциал для их использования в сельском хозяйстве в качестве агента биопрепарата для защиты растений комплексного действия.

Although the entomopathogenic bacteria Bacillus thuringiensis Berliner are well-known bio-agents for regulating the population of leaf-eating pests of agricultural and ornamental crops, other agricultural properties of this microorganism are promising. In this work, the growth-stimulating effect of entomopathogenic strains of B. thuringiensis on Deviz pea plants was studied. The entomopathogenic strains B. thuringiensis 685, 926 and 109-C obtained from the Crimean Collection of Microorganisms of the Crimean Agricultural Research Institute, registered online (http://www.ckp-rf.ru) with number 507484, were used as research material. The morphometric parameters of pea sprouts were evaluated following standard methods. Biochemical parameters of pea sprouts were determined for 10-day-old sprouts. The amylase activity of pea sprouts was determined by photocalorimetry; total acidity was measured by titration using 0.1 n NaOH solution; total water-soluble phenolic compounds were determined by Leventhal titrimetric method. It was found that liquid spore culture of strains B. thuringiensis 685, 926 and 109-C had a stimulating effect on the length of root and stem and the weight of 10-day-old sprouts of Deviz pea variety. Treatment with a spore suspension of all tested strains B. thuringiensis led to an increase in the content of organic acids in the sprouts by an average of 12.4% compared with that of the control. Maximum stimulating effect on amylolytic activity and synthesis of phenolic compounds in Deviz pea sprouts was achieved by treating with a spore suspension of the strain B. thuringiensis 926. The amylase activity increased on average by 41.5% when compared to that of the control, while the total content of phenolic compounds in this experiment was 2.3 times higher than that of the control. Therefore, in light of the entomopathogenic properties and the obtained data on the growth-stimulating activity of the strains of B. thuringiensis, it can be concluded that these bacteria have additional potential for their use in agriculture as a bio-agent for plant protection having a complex action.

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The authors declare that there are no conflicts of interest present.