vuzbiochemiИзвестия вузов. Прикладная химия и биотехнологияProceedings of Universities. Applied Chemistry and Biotechnology2227-29252500-1558ИРНИТУ10.21285/2227-2925-2022-12-4-589-598vuzbiochemi-902Research ArticleФИЗИКО-ХИМИЧЕСКАЯ БИОЛОГИЯPHYSICOCHEMICAL BIOLOGYРаспространение азоттрансформирующих бактерий в искусственном водоеме с высшими водными растениямиDistribution of nitrogen-transforming bacteria in an artificial reservoir populated with aquatic higher plantshttps://orcid.org/0000-0003-0091-5369НамН.В.Т.NamN.V.T.

Нгуен Ван Тхань Нам - магистр, научный сотрудник.

740300, Хошимин, ул. Третьего Февраля, район 10

Nguyen V. T. Nam - Master Sci., Researcher.

3, 3 Thang 2 St., 740300, Ho Chi Minh City, Distr. 10

xungcavn@gmail.comhttps://orcid.org/0000-0002-6035-5933ЛыонгТ.М.LuongM.T.

Тхи Мо Лыонг - кандидат химических наук, заведующий лабораторией.

740300, Хошимин, ул. Третьего Февраля, район 10

Mo T. Luong - Cand. Sci. (Chemistry), Head of the Laboratory.

3, 3 Thang 2 St., 740300, Ho Chi Minh City, Distr. 10

luongmo@mail.ruhttps://orcid.org/0000-0002-4054-9642ЮдинаН. Ю.YudinaN.Yu.

Наталья Юрьевна Юдина - кандидат химических наук, доцент, старший научный сотрудник.

300012, Тула, пр-т Ленина, 92

Natalia Yu. Yudina - Cand. Sci. (Chemistry), Researcher.

Tula State University, 92, Lenin Ave., Tula, 300012

TSUnewIT@gmail.comhttps://orcid.org/0000-0003-3481-2552ПонамореваО. Н.PonamorevaO. N.

Ольга Николаевна Понаморева - доктор химических наук, заведующий кафедрой биотехнологии.

300012, Тула, пр-т Ленина, 92

Olga N. Ponamoreva - Dr. Sci. (Chemistry), Head of the Department of Biotechnology.

92, Lenin Ave., Tula, 300012

olgaponamoreva@mail.ruhttps://orcid.org/0000-0002-5217-7815АлферовС. В.AlferovS. V.

Сергей Валерьевич Алферов - кандидат химических наук, заведующий лабораторией.

300012, Тула, пр-т Ленина, 92

Sergey V. Alferov - Cand. Sci. (Chemistry), Head of the Laboratory.

92, Lenin Ave., Tula, 300012

s.v.alferov@gmail.comhttps://orcid.org/0000-0002-1641-9418ЧиЧ.Н.Л.ChiT.N.L.

Чан Нгок Линь Чи - инженер, научный сотрудник.

740300, г. Хошимин, ул. Третьего Февраля, район 10

Tran N. L. Chi - Engineer, Researcher.

3, 3 Thang 2 St., 740300, Ho Chi Minh City, Distr. 10

tryphena6896@gmail.comhttps://orcid.org/0000-0001-5567-9725ЗунгН.Т.DungN.T.

Нгуен Тхи Зунг - студент, лаборант.

740300, Хошимин, ул. Третьего Февраля, район 10

Nguyen T. Dung - Student, Laboratory Assistant.

3, 3 Thang 2 St., 740300, Ho Chi Minh City, Distr. 10

dungnguyen14791@gmail.comhttps://orcid.org/0000-0002-7469-4004ЗуенФ.Т.М.DuyenPh.T.M.

Фунг Тхи Ми Зуен - студент.

720400, Хошимин, ул. П. Линь Трунг, квартал 6

Phung T. M. Duyen - Student, Nong Lam University,

16126039@st.hcmuaf.edu.vnhttps://orcid.org/0000-0003-2740-9853НгиаВ.Т.NghiaV.T.

Во Тхань Нгиа - студент.

720400, Хошимин, ул. П. Линь Трунг, квартал 6

Vo T. Nghia, Student - Nong Lam University.

6, P. Linh Trung St., 720400, Ho Chi Minh City

16126110@st.hcmuaf.edu.vnСовместный Российско-Вьетнамский Тропический научно-исследовательский и технологическтй центр, Южное отделениеВьетнамSouthern Branch of Joint Vietnam-Russia Tropical Science and Technology Research CenterViet NamТульский государственный университетРоссияTula State UniversityRussian FederationАграрно-лесной университетРоссияNong Lam UniversityRussian Federation202202012023124589598Copyright © Нам Н., Лыонг Т., Юдина Н.Ю., Понаморева О.Н., Алферов С.В., Чи Ч., Зунг Н., Зуен Ф., Нгиа В., 20232023Нам Н., Лыонг Т., Юдина Н.Ю., Понаморева О.Н., Алферов С.В., Чи Ч., Зунг Н., Зуен Ф., Нгиа В.Nam N., Luong M., Yudina N., Ponamoreva O.N., Alferov S.V., Chi T., Dung N., Duyen P., Nghia V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vuzbiochemi.elpub.ru/jour/article/view/902

Данная статья посвящена исследованию распространения азоттрансформирующих бактерий в искусственном водоеме с высшими водными растениями. Сконструированы искусственные водоемы (пруды) с высшими водными растениями тростника обыкновенного (Phragmites australis) и рогоза (Typha) для очистки свиноводческих сточных вод. В пруду с Phragmites australis выделено 7 штаммов аммонийокисляющих и 14 денитрифицирующих бактерий, а в пруду с Typha – 6 штаммов аммонийокисляющих и 19 денитрифицирующих бактерий. В результате сравнительного анализа численности бактерий в разных точках отбора образцов выявлено их снижение вдоль искусственного водоема. Больше всего аммонийокисляющих штаммов бактерий было идентифицировано в образцах, отобранных в поверхностном слое пруда, в то время как денитрифицирующие бактерии доминировали в донном слое. Выделенные микроорганизмы установлены методом определения последовательности нуклеотидов 16 SrRNA, они относятся к родам Pantoea, Enterobacter, Bacillus. Показано, что искусственный водоем с высшими водными растениями обладает разнообразной микробиотой, состав которой сильно зависит от источника сточных вод и не зависит от видов посаженных растений. Проведено определение способности трансформации аммиака и денитрификации выделенными штаммами бактерий. Наибольшая эффективность превращения аммиака (до 56%) показана для бактерий Enterobacter cloacae, выделенных из поверхностного слоя пруда. Денитрифицирующие бактерии, отобранные из донного слоя, способны снижать содержание нитрата с 20 до 10 мг/л за 72 ч. Полученные результаты подтверждают роль бактерий в очистке сточных вод от азотсодержащих загрязняющих веществ, а средняя эффективность удаления неорганических соединений азота составляет 50%.

This article examines the distribution of nitrogen-transforming bacteria in an artificial reservoir (pond) populated with aquatic higher plants of common reed (Phragmites australis) and cattail (Typha) for treating swine wastewater. In the pond occupied by Phragmites australis, 7 strains of ammonium oxidising and 14 denitrifying bacteria were identified, while, in the pond occupied by Typha, 6 strains of ammonium oxidising and 19 denitrifying bacteria were distinguished. A comparative analysis of bacterial count at various sampling points revealed their decrease along the artificial pond. Most of the bacteria strains oxidising ammonium were identified in the samples collected from the surface layer of the pond, while denitrifying bacteria dominated the bottom layer. The isolated microorganisms identified by 16S rRNA sequencing belonged to the genus Pantoea, Enterobacter and Bacillus. An artificial pond having aquatic higher plants is characterised by a diverse microbiota, whose composition strongly depends on the wastewater source rather than on the cultivated plant species. The ammonia transformation and denitrification capacity of isolated bacterial strains was determined. The highest conversion efficiency of ammonia (up to 56%) was observed for Enterobacter cloacae bacteria isolated from the surface layer of the pond. Denitrifying bacteria sampled from the bottom layer allowed for the reduction in nitrate content from 20 to 10 mg/L in 72 h. The obtained results confirm the role of bacteria in the treatment of wastewater against nitrogen-containing pollutants, with the average efficiency of removal of inorganic nitrogen compounds being 50%.

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The authors declare that there are no conflicts of interest present.