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Effect of endophytic bacteria Bacillus subtilison metabolic processes in potato tubers during storage

https://doi.org/10.21285/2227-2925-2023-13-4-569-578

EDN: CLFIST

Abstract

The article examines the effect produced by the preplant inoculation of potato tubers with Bacillus subtilis on biochemical processes occurring in the tubers of a new crop during the postharvest period at different storage periods. The content of malondialdehyde in tubers treated with strains 10-4 and 26D decreased by 15.5 and 35.5% and by 14.9 and 13.6% relative to the control after one and six months, respectively. The proline content in tubers pretreated with strain 10-4 differed insignificantly from the control in both storage periods, while in the case of strain 26D, it was lower than the control by 14.7 and 26.3%. First, the activity of protective proteins in treated tubers decreased relative to the control; then, the activity of trypsin inhibitors in tubers was found to significantly exceed that in tubers stored for a short time. The protein content of pretreated tubers was initially higher than that of the control; then, it was either at the same level (strain 26D) or 15% lower than the control (strain 10-4). The content of ascorbic acid in tubers pretreated with strain 10-4 exceeded that of the control by 16.1 and 17.9% after one and six months of storage, respectively, whereas in the case of strain 26D, no difference from the control was noted. The amount of reducing sugars was 39.4% lower than the control after one and six months of storage (strain 10-4) and 35.6% after six months of storage (strain 26D). The pretreatment with strains 10-4 and 26D positively affected the main biochemical parameters of tubers during long-term storage, providing a protective effect and preserving the biological value of produce.

About the Authors

L. I. Pusenkova
Bashkir Research Institute of Agriculture of Ufa Federal Research Centre RAS
Russian Federation

Ludmila I. Pusenkova, Cand. Sci. (Agriculture), Associate Professor, Leading Researcher

9, Rikhard Zorge St., Ufa, 450059



S. R. Garipova
Bashkir Research Institute of Agriculture of Ufa Federal Research Centre RAS; Ufa University of Science and Technology
Russian Federation

Svetlana R. Garipova, Dr. Sci. (Biology), Associate Professor, Leading Researcher; Professor

9, Rikhard Zorge St., Ufa, 450059

32 Zaki Validi St., Ufa, 450076



O. V. Lastochkina
Institute of Biochemistry and Genetics of Ufa Federal Research Centre RAS
Russian Federation

Oksana V. Lastochkina, Cand. Sci. (Biology), Senior Researcher

71, Oktyabrya Ave., Ufa, 450054



I. A. Shpirnaya
Ufa University of Science and Technology
Russian Federation

Irina A. Shpirnaya, Cand. Sci. (Biology), Associate Professor

32 Zaki Validi St., Ufa, 450076



V. A. Valieva
Bashkir Research Institute of Agriculture of Ufa Federal Research Centre RAS
Russian Federation

Valeria A. Valieva, Acting Junior Researcher

9, Rikhard Zorge St., Ufa, 450059



References

1. Uskov A.I., Kravchenko D.V., Uskova L.B., Galushka P.A., Zamyatnin A.A., Skulachev M.V. Regulation of physiological aging of potato culture. Zemledelie. 2019;8:33-37. (In Russian). DOI: 10.24411/0044-3913-2019-10808.

2. Ávila-Valdés A., Quinet M., Lutts S., Martínez J. P., Lizana X.C. Tuber yield and qualityresponses of potato to moderate temperatureincrease during Tuber bulking under two wateravailability scenarios // Field Crops Research. 2020. Vol. 251. P. 107786. DOI: 10.1016/j.fcr.2020.107786.

3. Belov G.L., Zeyruk V.N., Maltsev S.V., Abashkin O.V., Abrosimov D.V. Protection of potato during storage with chemical and biological preparations. Agrokhimicheskiy vestnik = Agrochemical Herald. 2020;6:75-78. (In Russian). DOI: 10.24411/1029-2551-2020-10090. EDN: DRZOJK.

4. Draie R., Al-Absi M. Regulation and control of potato tuber dormancy and sprouting. International Journal of Recent Advances in Multidisciplinary Research. 2019;6(1):4573-4583.

5. Fedotova L.S., Timoshina N.A., Knyazeva E.V. Dynamics of potato biochemical indicators during storage. Kartofel’ i ovoshchi = Potato and Vegetables. 2017;1:31-34. (In Russian). EDN: XIROXT.

6. Elmore J.S., Briddon A., Dodson A.T., Muttucumaru N., Halford N.G., Mottram D.S. Acrylamide in potato crisps prepared from 20 UK-grown varieties: effects of variety and tuber storage time. Food Chemistry. 2015;182:1-8. DOI: 10.1016/j.foodchem.2015.02.103.

7. Uskov A.I., Uskova L.B., Kravchenko D.V., Galushka P.A., Zakabunina E.N. Regulation of Physiological Aging Processes during Long-Term Potato Reproduction. Zemledelie. 2018;5:40-44. (In Russian). DOI: 10.24411/0044-3913-2018-10511.

8. Chebotar V.K., Kiprushkina E.I. Application of microbial preparations in potato storage technologies. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of AIC. 2015;29(1):33-35. (In Russian). EDN: TMZFSZ.

9. Novikova I.I., Titova Yu.A., Boikova I.V., Zeiruk V.N., Krasnobayeva I.L. Biological efficiency of new biopreparations based on antagonistic microbes in control of potato pathogens during plant vegetation and tuber storage. Biotekhnologiya. 2017;33(6):68-76. (In Russian). DOI: 10.21519/0234-27582017-33-6-68-76. EDN: YMVEAK.

10. Lastochkina O., Aliniaeifard S., Seifikalhor M., Yuldashev R., Pusenkova L., Garipova S. Plant growthpromoting bacteria: biotic strategy to cope with abiotic stresses in wheat. In: Hasanuzzaman M., Nahar K., Hossain Md.A. (eds). Wheat production in changing environments. Singapore: Springer; 2019, p. 579-614. DOI: 10.1007/978-981-13-6883-7_23.

11. Maksimov I.V., Pusenkova L.I., Abizgildina R.R. Biopreparation with endophytic bacterium Bacillus subtilis 26D created posthavest protecting effect in potato tubers. Agrokhimiya. 2011;6:43-48. (In Russian). EDN: NURDSX.

12. Lastochkina O., Pusenkova L., Garshina D., Yuldashev R., Shpirnaya I., Kasnak C., et al. The effect of endophytic bacteria Bacillus subtilis and salicylic acid on some resistance and quality traits of stored Solanum tuberosum L. tubers infected with fusarium dry rot. Plants. 2020;9(6):738. DOI: 10.3390/plants9060738.

13. Heath R.L., Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 1968;125(1):189-198. DOI: 10.1016/0003-9861(68)90654-1.

14. Bates L.S., Waldern R.P., Teare I.D. Rapid determination of free proline for water-stress studies. Plant and Soil. 1973;39:205-207. DOI: 10.1007/BF00018060.

15. Gofman Yu.Ya., Vaisblai I.M. Determination of trypsin inhibitor in pea seeds. Prikladnaya biokhimiya i mikrobiologiya. 1975;5:777-783. (In Russian).

16. Erlanger B.F., Kokowski N., Cohen W. The preparation and properties of two new chromogenic substrates of trypsin. Archives of Biochemistry and Biophysics. 1961;95(2):271278. DOI: 10.1016/0003-9861(61)90145-X.

17. Vasanthan T., Bergthaller W., Driedger D., Yeung J., Sporus P. Starch from Alberta potatoes: wet Isolation and some physicochemical proprieties. Food Research International. 1999;32(5):355-365. DOI: 10.1016/S0963-9969(99)00096-4.

18. Shcherbakov A.V., Shcherbakova E.N., Mulina S.A., Rots P.Yu., Daryu R.F., Kiprushkina E.I., et al. Psychrophilic endophytic pseudomonas as potential agents in biocontrol of phytopathogenic and putrefactive microorganisms during potato storage. Sel’skokhozyaistvennaya biologiya = Agricultural Biology. 2017;52(1):116-128. (In Russian). DOI: 10.15389/agrobiology.2017.1.116rus. EDN: YFQFFJ.

19. Garipova S.R., Markova O.V., Fedorova K.A., Dedova M. A., Kamaletdinova A. A., Lastochkina O. V., et al. Malondialdehyde and proline content in bean cultivars following the inoculation with endophytic bacteria. Acta Physiologiae Plantarum. 2022;44:89. DOI: 10.1007/s11738-022-03427-1.

20. Yarullina L.G., Akhatova A.R., Kasimova R.I. Hydrolytic enzymes and their proteinaceous inhibitors in regulation of plant–pathogen interactions. Fiziologiya rastenii. 2016;63:2:205-217. (In Russian). DOI: 10.7868/S0015330316020159. EDN: VINZCF.

21. Sorokan A.V., Burkhanova G.F., Alekseev V. Yu., Gordeev A.A., Mardanshin I.S., Maksimov I.V. Simultaneous effect of Bacillus thuringiensis B-5351 and salicylic acid on the resistance of potato plants to Phytophthora infestans and yield in the field. Biomics. 2023;15(1):1-6. (In Russian). DOI: 10.31301/2221-6197. bmcs.2023-1. EDN: NSIMDV.

22. Yarullina L.G., Veselova S.V., Ibragimov R.I., Shpirnaya I.A., Kasimova R.I., Akhatova A.R., et al. Tsvetkov V., Maksimov I. Search for molecular markers of wheat resistance to fungal pathogens. Agricultural Sciences. 2014;5:722-729. DOI: 10.4236/as.2014.58076.

23. Hao Y., Wu H., Liu Y., Hu Q. Mitigative effect of Bacillus subtilis QM3 on root morphology and resistance enzyme activity of wheat root under lead stress. Advances in Microbiology. 2015;5(6):469-478. DOI: 10.4236/aim.2015.56048.

24. Lastochkina O., Aliniaeifard S., Garshina D., Garipova S., Pusenkova L., Allagulova Ch., et al. Seed priming with endophytic Bacillus subtilis strain-specifically improves growth of Phaseolus vulgaris plants under normal and salinity conditions and exerts anti-stress effect through induced lignin deposition in roots and decreased oxidative and osmotic damage. Journal of Plant Physiology. 2021;263:153462. DOI: 10.1016/j.jplph.2021.153462.

25. Lastochkina O., Pusenkova L., Garshina D., Kasnak C., Palamutoglu R., Shpirnaya I., et al. Improving the biocontrol potential of endophytic bacteria Bacillus subtilis with salicylic acid against Phytophthora infestanscaused postharvest potato tuber late blight and impact on stored tubers quality. Horticulturae. 2022;8(2):117. DOI: 10.3390/horticulturae8020117.


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For citations:


Pusenkova L.I., Garipova S.R., Lastochkina O.V., Shpirnaya I.A., Valieva V.A. Effect of endophytic bacteria Bacillus subtilison metabolic processes in potato tubers during storage. Proceedings of Universities. Applied Chemistry and Biotechnology. 2023;13(4):569-578. (In Russ.) https://doi.org/10.21285/2227-2925-2023-13-4-569-578. EDN: CLFIST

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