THE USE OF NANOFILAMENTS BASED ON POLYVINYLPYRROLIDONE FOR THE IMMOBILIZATION OF LACTOBACILLUS ACIDOPHILUS CELLS
https://doi.org/10.21285/2227-2925-2018-8-2-69-76
Abstract
This study investigates the possibility of using polyvinylpyrrolidone (PVP) nanofilaments for the immobilization of Lactobacillus acidophilus strain Ep 317/402 n. v. Narine AAA. Two types of nanofilaments - PVPI and PVPII - with a diameter of 200-300 nm obtained by the electrospinning method were experimentally tested. The former type consisted in pure high-molecular polyvinylpyrrolidone nanofilaments, whereas the latter additionally included zinc oxide. The electrospinning unit consisted of a NE-300 syringe pump and a high-voltage INVER-30/5 source for producing an electric field of 1.8 kV/cm. The effectiveness of the immobilization using polyvinylpyrrolidone was evaluated according to the condition and morphology of Acidophilic Lactobacilli cells, their growth rate and the culture medium pH. The PVP modification with zinc oxide is shown to stimulate the acid resistance of probiotic bacteria and to cause the formation of a stable biofilm from exogenous metabolites. Scanning electron microscopy was used to establish the heterogeneous character of the exometabolic matrix, which predominantly consists of extracellular fibres temporarily held on the bacterial cell surface. The obtained results confirm the prospects of using PVP nanofilaments and their modifications for the optimization of probiotic immobilization technologies.
About the Authors
O. Ya. Berezina
Petrozavodsk State University
Russian Federation
Russian Federation
N. P. Markova
Petrozavodsk State University
Russian Federation
Russian Federation
A. V. Semenov
Petrozavodsk State University
Russian Federation
Russian Federation
N. A. Sidorova
Petrozavodsk State University
Russian Federation
Russian Federation
References
1. Blecher L., Lorenz D.H., Lowd H.L., Wood A.S., Wyman D.P. Polyvinylpyrrolidone // Davidson RL (ed) Handbook of water-soluble gums and resins. McGraw-Hill. 1980. New York. Р. 211-217.
2. Hong Y., Legge R.L., Zhang S., Chen P. Effect of amino acid sequence and pH on nanofiber formation of self-assembling peptides EAK16-II and EAK16-IV // Biomacromolecules.2003. V.4. N 5. Р. 1433-1442.
3. Ma P.X., Zhang R. Synthetic nano-scale fibrous extracellular matrix // J. of Biomedical Materials Research. 1999. V.46, N.1, pp 60-72.
4. Ellison C.J., Phatak A., Giles D.W. et. al. Melt blown nanofibers: Fiber diameter distributions and onset of fiber breakup // Polymer. 2007. Vol. 48. N.20. P. 6180-6187.
5. Doshi J., Reneker D.H. Electrospinning process and applications of electrospun fibers // J. of Electrostatics.1995. V.35. N.23. P. 151-160.
6. Li D., Wang Y., Xia, Y. Electrospinning nanofibers as uniaxially aligned arrays and layer-by-layer stacked films //Advanced Materials. 2004. V.16. N.4. P. 361-366.
7. Xue Y., Wang H., Yu D., Feng L., Dai L., Wang X., Lin T. Superhydrophobic electrospun POSS-PMMA copolymer fibers with highly ordered nanofibrillar and surface structures // Chemical Communications. 2009. N.42. P. 6418-6420.
8. Fang J., Wang H., Niu H., Lin T. et al. Evolution of fiber morphology during electrospinning //J. of Applied Polymer Science. 2010. V.118, N.5. P. 2553-2561.
9. Milosavljevic V., Jelinkova P., Jimenez A.- M., Moulick A. et. al. Alternative Synthesis Route of Biocompatible Polyvinylpyrrolidone Nanoparticles and Their Effect on Pathogenic Microorganisms // Mol. Pharmaceutics. 2017. N 14 (1). P. 221-233.
10. Zhao Y., Hu X., Li Z., Wang F., Xia Y. et al. Use of polyvinylpyrrolidone-iodine solution for sterilisation and preservation improves mechanical properties and osteogenesis of allografts // Scientiphic Reports. 2016. N 6. P. 107-113. DOI:10.1038/srep38669.
11. Sriwilaijaroen N., Wilairat P. et. аl. Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities // Virol. J. 2009. V.6. P. 132-144. DOI: 10.1186/1743-422X-6-124.
12. Pyar H., Peh K. K. Enteric coating of granules containing the probiotic Lactobacillus acidophilus // Acta Pharm. 2014. Jun; 64(2). P. 247-256.
13. Stummera S., Salar-Behzadia S., Ungera Frank M., Oelzantb S., Penningcelmut M., Viernsteina Н. Application of shellac for the development of probiotic formulations // Food Research International. 2009. N 43 (2010). P. 1312-1320.
Review
For citations:
Berezina O.Ya., Markova N.P., Semenov A.V., Sidorova N.A. THE USE OF NANOFILAMENTS BASED ON POLYVINYLPYRROLIDONE FOR THE IMMOBILIZATION OF LACTOBACILLUS ACIDOPHILUS CELLS. Proceedings of Universities. Applied Chemistry and Biotechnology. 2018;8(2):69-76. (In Russ.) https://doi.org/10.21285/2227-2925-2018-8-2-69-76
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