IDENTIFICATION OF A LIMITING FACTOR IN THE REDUCTION OF IODONITROTETRAOLIUM CHLORIDE BY A BACTERIAL CELL SUSPENSION IN A PHYSIOLOGICAL SOLUTION

In this work, we set out to investigate the role of diffusion in the microbial reduction of iodonitrotetraolium chloride (INT) using the methods of chemical kinetics. In environmental studies, re-searchers use the intensity of tetrazolium salt reduction to evaluate an overall viability of microorganisms and their communities. In particular, tetrazolium salts could be used as an indicator of the corrosive activity of bacteria. However, it is known that the reduction of tetrazolium salts can only register the response of those microbial community parts that are capable of actively reducing these substances. Therefore, research into the causes of a wide-range reduction ability of microorganisms towards tetrazolium salts can improve the objectivity of environmental and other studies, including the study of the corrosion activity of organotrophic bacteria. The kinetic parameters of INT reduction by Clostridium spp., Proteus vulgaris and Escherichia coli suspensions have been determined. On the basis of the values of the pre-exponential factor in the Arrhenius equation, an assumption is made that the reaction is characterized by a diffusion-controlled character.

iron, hydrogen peroxide, Fenton reaction, oxywater, oxene, pyridoxine

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