Naphthalene concentration dynamics in an aqueous medium in the presence of Bacillus megaterium
https://doi.org/10.21285/achb.950
EDN: XFPEVA
Abstract
The study examined the concentration dynamics of naphthalene, with its initial concentrations of 1, 2, and 3 g/L in synthetic wastewater. The initial number of Bacillus megaterium MK64-1 cells in the medium amounted to 1.7×107 CFU/mL. On day 14 of the experiment, the concentration of naphthalene decreased to hundredths of a gram, while the microbial count increased to 109 CFU/mL (at the initial naphthalene concentrations of 1 and 2 g/L) and 1011 CFU/mL (at the initial naphthalene concentration of 3 g/L). After 14 days, the medium pH decreased by an average of 0.7 units (from 8.56 to 7.86) in both test and control media, with the addition of a microbial suspension. During this time, the redox potential of the medium increased by an average of 70 mV in the test media. Quite a strong direct correlation (p < 0.05) was found between the initial concentration and the amount of pollutant degraded by bacteria. The determination of dehydrogenase activity in Bacillus megaterium by means of two methods (with 2,3,5-triphenyltetrazolium chloride and methylene blue), as well as microbial sensitivity to hydrocarbon concentrations of 1, 2, and 3 g/L via the disk-diffusion method, showed no toxic effect of the analyzed pollutant concentrations on bacteria under the experimental conditions. The obtained results indicate the ability of Bacillus megaterium strain MK64-1 to biodegrade naphthalene.
Keywords
Supporting agencies: The Department of Education and Science of Khanty-Mansi Autonomous Okrug – Yugra supported this work (Grant no. 2023-227-05).
About the Authors
S. A. Kovalenko
Baikal Museum SB RAS
Russian Federation
Russian Federation
Svetlana A. Kovalenko, Postgraduate Student
1, Academic St., Listvyanka, 664520
A. B. Kupchinsky
Baikal Museum SB RAS
Russian Federation
Russian Federation
Alexander B. Kupchinsky, Cand. Sci. (Biology), Director
1, Academic St., Listvyanka, 664520
D. I. Stom
Baikal Museum SB RAS ; Surgut State University ; Irkutsk State University ; Irkutsk National Research Technical University
Russian Federation
Russian Federation
Devard I. Stom, Dr. Sci. (Biology), Professor, Chief Researcher
1, Akademicheskaya St., Listvyanka, 664520
1, Lenin Ave., Surgut, 628412
1, Karl Marx St., Irkutsk, 664003
83, Lermontov St., Irkutsk, 664074
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Review
For citations:
Kovalenko S.A., Kupchinsky A.B., Stom D.I. Naphthalene concentration dynamics in an aqueous medium in the presence of Bacillus megaterium. Proceedings of Universities. Applied Chemistry and Biotechnology. 2024;14(4):548-555. (In Russ.) https://doi.org/10.21285/achb.950. EDN: XFPEVA
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