ASSESSMENT OF PROTEIN ENCAPSULATION AND ISOLATION FROM MICROPARTICLES CONTAINING FUCOIDAN UNDER THE ACTION OF SURFACTANTS
https://doi.org/.org/10.21285/2227-2925-2018-8-3-92-98
Abstract
In this work, we investigate the process of microparticle formation using highly-sulphated polysaccharides isolated from brown algae (Fucoidan) and protein (bovine serum albumin) under the action of acrylate emulsion and non-ionic surfactants. Polysaccharide/protein conjugates were formed as a result of electrostatic interaction between the biopolymers at their different ratios in an acidic medium (pH 4,5). Subsequently, the non-ionic silicone emulsifier PEG-12 Dimethicone was added. A gel based on acrylate emulsion was used as the carrier of such particles. The microparticles obtained are shown to exhibit high levels of stability, load efficiency and encapsulation. A dependence between these parameters and the quantitative ratio of the biopolymers used is established. It is shown that the particle size does not exceed 330 nm. The lowest particle size values were achieved under the action of a low-concentrated Fucoidan solution. It is determined that particles with a 1:1,5 ratio of protein/polysaccharide and an average size of 285 nm show the greatest encapsulating ability.
About the Authors
V. E. Suprunchuk
North Caucasian Federal University
Russian Federation
Russian Federation
E. V. Denisova
North Caucasian Federal University
Russian Federation
Russian Federation
S. F. Andrusenko
North Caucasian Federal University
Russian Federation
Russian Federation
T. P. Bondar
North Caucasian Federal University
Russian Federation
Russian Federation
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Review
For citations:
Suprunchuk V.E., Denisova E.V., Andrusenko S.F., Bondar T.P. ASSESSMENT OF PROTEIN ENCAPSULATION AND ISOLATION FROM MICROPARTICLES CONTAINING FUCOIDAN UNDER THE ACTION OF SURFACTANTS. Proceedings of Universities. Applied Chemistry and Biotechnology. 2018;8(3):92-98. (In Russ.) https://doi.org/.org/10.21285/2227-2925-2018-8-3-92-98
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