Resistance of bacterial biofilms comprising activated sludge microbial communities to physicochemical external factors
https://doi.org/10.21285/achb.1010
EDN: AFVEBZ
Abstract
The study was aimed at evaluating the resistance of Alcaligenes faecalis 2, Achromobacter pulmonis, Paenibacillus odorifer, and Bacillus subtilis biofilms to negative physicochemical external factors during batch culture. The main analyzed parameters included temperature (10 and 50 °С) and pH level (5.0 and 10.0), as well as surfactant (sodium dodecyl sulfate) concentrations of 5, 10, and 50 mg/dm3. At low temperatures (10 °С), the size of Alcaligenes faecalis 2, Achromobacter pulmonis PNOS, and Bacillus subtilis biofilms was found to increase. The Alcaligenes faecalis 2 biofilm was noted to exhibit resistance and metabolic activity under acidic conditions. A pH rise to 10.0 resulted in a higher amount of Bacillus subtilis biofilm. When exposed to sodium dodecyl sulfate solution (5 to 10 mg/dm3), Alcaligenes faecalis 2, Bacillus subtilis, Achromobacter pulmonis PNOS, and Paenibacillus odorifer biofilms were observed to form. In response to the exposure to high sodium dodecyl sulfate concentrations (10 and 50 mg/dm3), the biomass and size of Achromobacter pulmonis PNOS biofilm remained unchanged. Thus, the considered cultures were shown to be stress-resistant to negative external factors, which may contribute to the resistance of microbial cultures to various types of pollutants in treatment technologies.
About the Authors
A. A. Khasanova
Kazan National Research Technological University
Russian Federation
Russian Federation
Aigul A. Khasanova, Postgraduate Student
68, Karl Marx St., Kazan, 420015
A. S. Sirotkin
Kazan National Research Technological University
Russian Federation
Russian Federation
Aleksandr S. Sirotkin, Dr. Sci. (Engineering), Professor, Head of the Department
68, Karl Marx St., Kazan, 420015
E. V. Perushkina
Kazan National Research Technological University
Russian Federation
Russian Federation
Elena V. Perushkina, Cand. Sci. (Engineering), Associate Professor
68, Karl Marx St., Kazan, 420015
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Khasanova A.A., Sirotkin A.S., Perushkina E.V. Resistance of bacterial biofilms comprising activated sludge microbial communities to physicochemical external factors. Proceedings of Universities. Applied Chemistry and Biotechnology. (In Russ.) https://doi.org/10.21285/achb.1010. EDN: AFVEBZ
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