Generation of hydrogen peroxide in potato plants and potato cell cultures under their infection with Pectobacterium carotovorum ssp. сarotovorum
https://doi.org/.org/10.21285/2227-2925-2019-9-1-67-74
Abstract
In this research, we investigated changes in the concentration of hydrogen peroxide in the potato plants and their cell cultures (Solanum tuberosum L.) of two varieties - Lugovskoy and Lukyanovsky - under the action of the necrotrophic bacterial phytopathogen Pectobacterium carotovorum ssp. сarotovorum (Pcc) and its exometabolites (filtrate devoid of bacterial cells and thermally inactivated bacterial suspension). A biphasic accumulation of hydrogen peroxide is shown to occur in the cell cultures of the potato varieties under investigation infected with Pcc It is established that a thermally inactivated Pcc culture affects both the cell cultures and plant roots of the plants under study by causing a significant change in the generation of hydrogen peroxide in comparison with a filtrate devoid of bacterial cells. When infected with pathogens and their exometabolites, the intensity and dynamics of changes in hydrogen peroxide in the potato plants is significantly higher compared to a suspension cell culture. The intensity and dynamics of changes in the generation of hydrogen peroxide indicate the development of a systemic induced resistance in the studied potato varieties to Pcc bacteria and their exometabolites.
About the Authors
S. V. Boyarkina
Siberian Institute of Plant Physiology and Biochemistry, Russian Academy of Sciences, Siberian Branch
Russian Federation
Russian Federation
Y. V. Omelichkina
Siberian Institute of Plant Physiology and Biochemistry, Russian Academy of Sciences, Siberian Branch
Russian Federation
Russian Federation
T. N. Shafikova
Siberian Institute of Plant Physiology and Biochemistry, Russian Academy of Sciences, Siberian Branch
Russian Federation
Russian Federation
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For citations:
Boyarkina S.V., Omelichkina Y.V., Shafikova T.N. Generation of hydrogen peroxide in potato plants and potato cell cultures under their infection with Pectobacterium carotovorum ssp. сarotovorum. Proceedings of Universities. Applied Chemistry and Biotechnology. 2019;9(1):67-74. (In Russ.) https://doi.org/.org/10.21285/2227-2925-2019-9-1-67-74
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