Optimisation of conditions for extracting bioactive compounds exhibiting antioxidant properties from hawthorn fruit (Crataegus)
https://doi.org/10.21285/2227-2925-2019-9-2-239-249
Abstract
Hawthorn berries are traditionally used as a medicinal raw material in folk medicine. This plant contains important organic acids and flavonoids, such as carotene, pectin, ascorbic acid, saponins, starch and group B vitamins, which are believed to facilitate the restoration of the cardiovascular system. Medicinal raw materials are primarily used in the form of extracts. In this work, we use three extraction technologies: one conventional technique, i.e. infusion (37 °С, 2 hours), and innovative ones involving the use of microwave (800 W, 1 min) and ultrasonic irradiation (0.5 W, 2 hours). In order to determine the most optimal method for obtaining a complex of substances exhibiting antioxidant properties from hawthorn fruit extracts, we studied the content of dry substances, phenols and flavonoids. In addition, the antioxidant activity was determined by trapping free radicals and using the FRAP method (restoring force). On the basis of the obtained experimental results, the hawthorn fruit extraction conducted using ultrasonic irradiation is found to be the most effective of all the considered technologies, since it yields the highest values for all the studied parameters as compared to other extraction methods. Thus, the following levels were achieved: phenols – 723 mg of gallic acid per 100 g; flavonoids – 194 mg of catechin per 100g; dry substances – 1.68 %; anti-radical activity – 14.5 mg/cm3 ; restoring force – 14.5 mmol of Fe2+ per 1 kg. Our results show that the extraction using microwave irradiation cannot be recommended, since the values obtained using this method are lower than those obtained by ultrasonic irradiation and infusion.
About the Authors
A. R. Valeeva
Samara State Technical University
Russian Federation
Russian Federation
Student,
Samara
N. V. Makarova
Samara State Technical University
Russian Federation
Russian Federation
Dr. Sci. (Chemistry), Head of Department of Technology and Organization of Public Catering,
Samara
D. F. Valiulina
Samara State Technical University
Russian Federation
Russian Federation
Ph.D. (Engineering), Associate Professor, Department of Technology and Organization of Public Catering,
Samara
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Review
For citations:
Valeeva A.R., Makarova N.V., Valiulina D.F. Optimisation of conditions for extracting bioactive compounds exhibiting antioxidant properties from hawthorn fruit (Crataegus). Proceedings of Universities. Applied Chemistry and Biotechnology. 2019;9(2):239-249. https://doi.org/10.21285/2227-2925-2019-9-2-239-249
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