STUDY OF POLYLAYER DIETARY FIBER BASED CAPSULES BEHAVIOR UNDER ENZYMATIC HYDROLYSIS IN VITRO

Abstract

The aim of this work was to study the behavior of single and multilayer capsules from sodium alginate and CMC during enzymatic hydrolysis in-vitro. Multilayer capsules based on the dietary fiber with encapsulated model biologically active protein were developed. Their behavior was studied under conditions of enzymatic hydrolysis in vitro using physical methods of analysis. It is shown that the capsules size was followed the reduction trends in the model "stomach." However, after being placed in an intestinal solution all capsules began to swell to varying degrees due to an increase in electrostatic repulsion forces. During the experiment, it was found that the hardness of the capsules in a model "stomach" was higher as compared to their mechanical properties in a model "intestine", followed by the destruction of capsules at the end of intestinal phase. It is noted that the multilayer capsule had the greatest ability to protect the encapsulated model biologically active protein in acidic gastric environment. The results of this analysis indicate that the changes in the mechanical properties and the swelling behavior of capsules based on dietary fiber provide a relationship to the control release of encapsulated bioactive components.