Comparative assessment of two calculation methods for change dynamics in the major fatty acid content of Triticum aestivum L. wheat calli under the action of low-intensity laser radiation
https://doi.org/10.21285/2227-2925-2019-9-3-439-446
Abstract
The aim of the present work involves a comparative analysis of the changes in the absolute content of the main fatty acid (FA) component of the total lipid amount in wheat calli (Triticum aestivum L.) under the action of low-intensity laser radiation (LILR), as well as an assessment of the influence of the FA proportion calculation method on the resultant picture of the FA change dynamics. The content of major FAs in callus tissue and its changes when exposed to LILR were determined using gas chromatography-mass spectrometry. A comparative analysis of the calculation results of the absolute (μg/g dry weight) and relative (in %wt. of the sum of the areas of chromatographic peaks) content of the target components (palmitic, stearic, linoleic and linolenic acids) was carried out. When comparing the two calculation methods, no qualitative differences in the change dynamics were observed. At the same time, significant differences were found between the quantitative indicators of the FA content in absolute and relative units. For example, the absolute amount of monounsaturated oleic acid five minutes increased by 23% following the removal of laser radiation, while the relative amount increased by only 14%. For polyunsaturated linoleic and linolenic acids, this difference was even more significant. In general, the trends in changes caused by irradiation in the main fatty acid content compared with the total callus lipids appear to be similar to the stress response of plant tissue to adverse environmental conditions. The scope of two methods for calculating the content of FAs is also discussed.
About the Authors
L. V. Dudareva
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation
Russian Federation
Ph.D. (Biology), Leading Researcher, Head of Laboratory, Physical and Chemical Research Methods Laboratory,
Irkutsk
V. N. Shmakov
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation
Russian Federation
Ph.D. (Biology), Senior Researcher, Plant Genetic Engineering Laboratory,
Irkutsk
E. G. Rudikovskaya
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation
Russian Federation
Ph.D. (Biology), Senior Researcher, Laboratory of Physiological and Biochemical Adaptation of Plants,
Irkutsk
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Review
For citations:
Dudareva L.V., Shmakov V.N., Rudikovskaya E.G. Comparative assessment of two calculation methods for change dynamics in the major fatty acid content of Triticum aestivum L. wheat calli under the action of low-intensity laser radiation. Proceedings of Universities. Applied Chemistry and Biotechnology. 2019;9(3):439-446. https://doi.org/10.21285/2227-2925-2019-9-3-439-446
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