Diffuse-reflection electron spectroscopy in the study of muscle tissue of wild and domestic animals
https://doi.org/10.21285/2227-2925-2019-9-3-489-499
Abstract
The aim of the work was to study the application of diffuse reflection electron spectroscopy (DRES) method for obtaining the optical surface characteristics of slices and chopped muscle tissue, muscle fibre and sarcoplasmic protein extracts of wild (wild boar, elk, deer, freshwater and marine fish) and domestic (pork, beef, lamb) animal tissue depending on the following processing conditions: freezing, water extraction, lyophilisation and enzyme-microbial exposure. Electronic spectra of transverse sections of whole-piece material, ground muscle tissue, muscle fibre and aqueous extracts were recorded using a Specord M-200 spectrophotometer (AIZ Engineering GmbH, Germany) having a vertical pathway relative to the Spectralon standard in the wavelength range of 200-700 nm. During testing of biological tissues of animal origin in solid and liquid state, the high sensitivity, resolution and analytical features of the express DRES method was shown to allow the acquisition of factual information concerning changes in the spectral characteristics of their main components under experimental conditions. Regardless of the species and type of animal organism, proteins, mucopolysaccharides, lipids, pigment protein and various oxidised forms demonstrate absorption bands in four clearly differentiated regions of the electromagnetic spectrum: 200-300, 400-425, 300-380, 540-580 and 635 nm, respectively. A comparative study of the pork and beef muscle tissue demonstrated significant differences in the nature of changes in the spectral characteristics of the main components and components of muscle tissue and muscle fibre, depending on the degree of oxidation, mechanical destruction, strength of myofibrils and pigment protein myoglobin associated with them.
About the Authors
A. P. Nechiporenko
National research University of information technologies, mechanics and optics
Russian Federation
Russian Federation
Dr. Sci. (Chemistry), Professor,
Saint Petersburg
S. M. Orehova
National research University of information technologies, mechanics and optics
Russian Federation
Russian Federation
Ph.D. (Chemistry), Head of the Laboratory of Chemical and Biological Class,
Saint Petersburg
L. V. Plotnikova
St. Petersburg State University
Russian Federation
Russian Federation
Senior Researcher,
Saint Petersburg
P. P. Plotnikov
National research University of information technologies, mechanics and optics
Russian Federation
Russian Federation
Student,
Saint Petersburg
References
1. Tselykhova E.K. Russkaya okhotnich'ya kukhnya. Istoriya [Russian hunting cooking. History]. Moscow: Veche Publ., 2016, 272 p.
2. Kriger-Mettbakh B. Rare types of meat. Exotic menu. Novoe myasnoe delo. 2006, no. 2, pp. 16–18. (In Russian)
3. Gerasimova N.Yu. Unconventional types of meat raw materials for the production of functional products. Izvestiya vuzov. Pishchevaya tekhnologiya. 2012, no. 2–3 (326–327), pp 17–20. (In Russian)
4. Berlova G.A. The meat of wild animals. Special rules, special recipes. Vse o myase. 2008, no 6, pp. 58–59. (In Russian)
5. Samchenko O.N. Use of meat of wild animals in meat products technology. Nauka i sovremennost'. 2013, no. 24, pp. 220–225. (In Russian)
6. Rudintseva T.A., Safronova G.A., Nechaeva N.G. Meat products for medical treatment and prevention. Myasnaya promyshlennost'. 1994, no. 6, pp. 26–27. (In Russian)
7. Khlebnikov V.I., Dmitrienko S.Yu. Quality of meat products with functional properties. Izvestiya vuzov. Pishchevaya tekhnologiya. 2004, no. 1 (278), pp. 67–68. (In Russian)
8. Zaporozhskii A.A., Kas'yanov G.I., Linets A.A. Use of a new type of raw meat in functional food production. Vse o myase. 2007, no. 3, pp. 8–9. (In Russian)
9. Chirich E.G., Babina M.P. Study of chemical composition and nutritional value of wild meat. Uchenye zapiski uchrezhdeniya obrazovaniya “Vitebskaya ordena “Znak pocheta” gosudarstvennaya akademiya veterinarnoi meditsiny”. 2014, vol. 50, no. 1–1, pp. 202–204. (In Russian)
10. Lisitsyn A.B., Tatulov Yu.V., Chernukha I.M., Mittelshtein T.M. World practice of forming the quality of meat raw materials and the requirements for the processing industry. Myasnaya industriya. 2001, no. 9, pp. 8–12. (In Russian)
11. Taeva A.M. Microstructural studies of Kazakh two-humped camel meat. Myasnaya industriya. 2016, no. 8, pp. 46–49. (In Russian)
12. Dusaev N.S., Grishin A.S. Dangerous but delicious brown bear. Myasnye tekhnologii. 2010, no. 1 (85), pp. 46–47. (In Russian)
13. Egorov A.A., Khurshudyan S.A. Modern methods of analysis in the food industry. Pishchevaya promyshlennost'. 2002, no. 9, pp. 68–69. (In Russian)
14. Silverstein R.M., Webster F.X., Kiemle D.J. Spectrometric Identification of Organic Compounds. John Willey & Sons, 2005. (Russ. ed. Sil'verstein R., Vebster F., Kiml D. Spektrometricheskaya identifikatsiya organicheskikh soedinenii. Moscow: BINOM. Laboratoriya znanii Publ., 2011, 557 p.)
15. Muñoz Morales A.A., Vázquez y Montiel S. Retrieving the optical parameters of biological tissues using diffuse reflectance spectroscopy and Fourier series expansions. I. theory and application. Biomedical Optics Express. 2012, vol. 3, no. 10, pp. 2395–2404. DOI: 10.1364/BOE.3.002395
16. Sokolov A.A. Fiziko-khimicheskie i biokhimicheskie osnovy tekhnologii myasoproduktov [Physico-chemical and biochemical foundations of meat technology]. Moscow: Pishchevaya promyshlennost' Publ., 1965, 489 p.
17. Rogov I.A., Zabashta A.G., Kazyulin G.P. Obshchaya tekhnologiya myasa i myasoproduktov [General technology of meat and meat products]. Moscow: Kolos Publ., 2000, 367 p.
18. Cheong W.F., Prahl S.A., Welch A.J. A review of the optical properties of biological tissues. IEEE Journal of Quantum Electronics. 1990, vol. 26, pp. 2166–2185.
19. Orekhova S.M., Nechiporenko A.P. The effect of pork muscle tissue treatment with ethanol on the optical characteristics of muscle fiber. Izvestiya vuzov. Pishchevaya tekhnologiya.2013, no. 4, pp.16–18. (In Russian)
20. Giraev K.M., Ashurbekov N.A., Kobzev O.V. Optical studies of biological tissues: determination of absorption and scattering. Pis'ma v ZhTF. 2003, vol. 29, issue 21, pp. 48–54. (In Russian)
21. Orehova S., Nechiporenko U., Vasileva I., Nechiporenko A. Ethanol effect on the radiolysis of pork muscle tissue. In: Proc. 6th Baltic Conf. on Food Sci. and Technol. Foodbalt 2011. “Innovation for food science and production”. Latvia, Jelgava, 2011, pp. 177–181.
22. Nechiporenko U.Yu., Plotnikova L.V., Mel'nikova M.I. Lipidy, ikh kupazhi, ekstrakty i shroty rastitel'nogo syr'ya [Lipids, their blends, extracts and meal of plant materials]. Lambert Academic Publishing, 2019, 156 p.
23. Beloveshkin A. Astaksantin (korol' antioksidantov): sila rozovogo tsveta i sekret flamingo [Astaxanthin (king of antioxidant): the power of pink and the secret of Flamingo]. Available at: http://www.beloveshkin.com/2015/09/astaksantinkorol-antioksidantov-sila-rozovogo-sekret-flamingo.html(accessed 19.04. 2019).
24. Demchenko A.P. Ul'trafioletovaya spektrofotometriya i struktura belkov [Ultraviolet spectrophotometry and protein structure]. Kiev: Naukova dumka Publ., 1981, 208 p.
Review
For citations:
Nechiporenko A.P., Orehova S.M., Plotnikova L.V., Plotnikov P.P. Diffuse-reflection electron spectroscopy in the study of muscle tissue of wild and domestic animals. Proceedings of Universities. Applied Chemistry and Biotechnology. 2019;9(3):489-499. (In Russ.) https://doi.org/10.21285/2227-2925-2019-9-3-489-499
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