Biosynthetic activity study of Lactobacillus acidophilus lactic acid bacteria in the lactose fermentation of whey

According to numerous studies, lactic acid bacteria are characterised by the capability of reducing their initial activity given an insufficient quantity of growth factors supplied by a nutrient medium. Conversely, the introduction of additional sources of nutrient into the medium provides favourable conditions for the development of lactic acid-producing microorganisms. The present study was aimed at examining the effect of phosphorus-containing salts on the biosynthetic activity of a specially selected strain of lactic acid bacteria for the biotransformation of acid whey lactose into lactate-containing ingredients. For this purpose, lactic acid bacteria of the Lactobacillus acidophilus thermophilic bacilli subgroup applied in cheese and fermented dairy production were used as a producer of lactic acid. In the obtained fermented solutions, the mass fractions of lactose and calcium lactate were determined by the Bertrand method and complexometric techniques, respectively. The variables in the biosynthetic activity study of lactic acid bacteria included the type of phosphoruscontaining salt (disodium phosphate dodecahydrate and disubstituted ammonium phosphate) and its mass fraction, which ranged from 1.0 to 3.0 % in increments of 0.5 %. The amount of inoculum introduced for maximum production of lactic acid comprised 2.5 % vol. of the nutrient medium. The titratable acidity of the inoculum ranged from 1.80 to 1.85 g/cm3 . In order to produce calcium lactate, the lactic acid accumulating during biosynthesis was neutralised with chalk. The effect caused by the type of phosphorus-containing salt and its mass fraction on the coefficient of whey bioconversion to lactic acid by L. аcidophilus AT-I lactic acid bacteria was evaluated along with the rate of formation and yield of calcium lactate. The 2.0 % additive of sodium phosphate disubstituted dodecahydrate was established to provide the highest values for the formation rate and yield of the target product, comprising 0.78 g/(dm3 ·h) and 79.96 %, respectively.

The authors declare no conflict of interests regarding the publication of this article.

1. Gorlov IF, Mosolova NI, Serova OP, Levina YaO, Lopaeva EA. Innovative Technology of Whey Processing Using Modified Starch. Khranenie i pererabotka sel’khozsyr’ya = Storage and Processing of Farm Products. 2018;2:58–61. (In Russian)

2. Asnovin MA. Zarubezhnyi opyt povysheniya effektivnosti ispol’zovaniya syvorotki = Foreign experience of increase of dairy whey use efficiency. In: Formirovanie organizatsionnoekonomicheskikh uslovii effektivnogo funktsionirovaniya APK: Sbornik nauchnykh statei 9-i Mezhdunarodnoi nauchno-prakticheskoi konferentsii = Formation of organizational and economic conditions for the effective functioning of agriculture: Proceedings of the 9th International Scientific and Practical Conference, 25–26 May 2017, Minsk: Belarusian State Agrarian Technical University, 2017, p. 85–89. (In Russian)

3. Volodin DN, Zolotoreva MS, Topalov VK, Evdokimov IA, Chablin BV. Features of cheese whey processing. Pererabotka moloka = Milk processing. 2017;3:6–8. (In Russian)

4. Lysenko YuA, Khusid SB, Volkova SA, Nikolaenko SN, Luneva AV, Nosenko AV. Development of a functional feed additive. Politematicheskii setevoi elektronnyi nauchnyi zhurnal Kubanskogo gosudarstvennogo agrarnogo universiteta = Polythematic online scientific journal of Kuban State Agrarian University. 2016;115:643–664. Available from: http://ej.kubagro.ru/archive.asp?n= 115 [Accessed 21th May 2019]. (In Russian)

5. Grigorash AI, Maklanov AI, Yurova EA. Complex utilization of whey – new opportunities to improve the efficiency of milk processing. Available from: https://rtpp.ru/old/news/2007/st07_007.htm 115 [Accessed 21th May 2019]. (In Russian)

6. Mel'nikova EI, Stanislavskaya EB. Whey ingredients: prospects of production and directions of use. Molochnaya reka = Milk river. 2019;2:52–56. (In Russian)

7. Gavrilov GB, Kravchenko EF. Ways of the efficient application of milk whey. Syrodelie i maslodelie = Cheesemaking and buttermaking. 2013;2:10–13. (In Russian)

8. Bologa MK, Vrabie EG, Stepurina TG. The peculiarities of mineralization of proteinmineral concentrates by electrophysical treatment of whey. Elektronnaya obrabotka materialov = Surface Engineering and Applied Electrochemistry. 2013;49:61–65. (In Russian)

9. Jelen P. Whey Processing: Utilization and Products. In: Fuquay JF., Fox PF., McSweeney PLH. (eds.) Encyclopedia of Dairy Sciences, vol. 4, 2nd ed. Academic, Oxford; p. 731–737. https://doi.org/10. 1016/B978-0-12-374407-4.00495-7

10. Zipaev DV, Zimichev AV. Whey is a valuable raw material for recycling. Izvestiya vuzov. Pishchevaya tekhnologiya = News of institutes of higher education. Food technology. 2007;2:14–16. (In Russian)

11. Grunskaya VA, Gabrielyan DS. Microbiological Aspects of Enriched Fermented Milk Products Manufacture with Use of Whey. Molochnokhozyaistvenny Vestnik. 2018;3:91–103. (In Russian)

12. Polyanskii KK, Shuvaeva GP, Yakovlev VF, Demenko NA. Membrane Technology in Calcium Lactate Producing from Whey. Biotekhnologiya = Biotechnology. 2000;2:37–41. (In Russian)

13. Khramtsov AG, Abilov BT, Lodygin DN, Lodygin AD, Kryuchkov PG. Method of producing combined feed concentrate. Patent RF, no. 2352138, 2007. (In Russian)

14. Ryabtseva SA, Skripnyuk AA, Kotova AA, Khramtsov AG, Rodnaya AB, Lodygin AD, et al. Novyi sposob polucheniya fermentnogo preperata beta-galaktozidaz drozhzhei i molochnokislykh bakterii = A new method for producing the enzyme preparation of yeast betagalactosidase and lactic acid bacteria. In: Molekulyarno-geneticheskie i biotekhnologicheskie osnovy polucheniya i primeneniya sinteticheskikh i prirodnykh biologicheski aktivnykh veshchestv: Materialy Mezhdunarodnoi nauchno-prakticheskoi konferentsii = Molecular-genetic and biotechnological bases of production and application of synthetic and natural biologically active substances: Proceedings of the International Scientific and Practical Conference, 20–23 September 2017, Minsk – Stavropol', Stavropol: North-Caucasus Federal University, 2017, p. 257–261. (In Russian)

15. Zlotnikov KM, Zlotnikov AK, Kaparullina EV, Kazakov AV, Kazakova ML. Phosphate solubilization activity of lactic acid bacteria. Vladimirskii zemledelets = Vladimir agricolist. 2012; 4(62):20–21. (In Russian)

16. Vinarov AYu, BelYakov YuI, Sidorenko TE, Karavatskii AI. Method of whey processing. Patent RF, no. 2154386, 2000. (In Russian)

17. Artyukhova SI, Motornaya EV. Actuality of the using lactic acid bacteria synthesizing exopolysaccharides in the production of bioproducts for functional nutrition. Mezhdunarodnyi zhurnal eksperimental'nogo obrazovaniya = International Journal of Experimental Education. 2015;5-1:76. (In Russian)

18. Markelova VV, Krasnikova LV. Synthesis of exopolysaccharides by the strains of L. acidophilus in milk whey. Izvestiya vuzov. Pishchevaya tekhnologiya = News of institutes of higher education. Food technology. 2013;4:26–29. (In Russian)

19. Botina SG, Rozhkova IV, Semenikhina VF. Use of strains produced EPS in manufacture of the sour-milk products. Khranenie i pererabotka sel'skokhozsyr'ya = Storage and Processing of Farm Products. 2010;1:38–40. (In Russian)

20. Krasnikova LV, Markelova VV, Verbitskaya NB, Dobrolezh OV. Functional whey products using antagonistically active strains of acidophilic lactobacilli. Izvestiya vuzov. Pishchevaya tekhnologiya = News of institutes of higher education. Food technology. 2012:1:41–43. (In Russian)

21. Sinel’nikov BM, Khramtsov AG, Evdokimov IA, Ryabtsev SA, Serov VA. Lactose and its derivatives. St. Petersburg: Professiya, 2007. (In Russian)