Isolation of the Inonotus obliquus chaga mushroom strain and intensification of a culture growth during solid-phase cultivation

The extracts from fungus Inonotus obliquus (Pers.:Fr.) Pilat (chaga), which have been widely used in medicine and cosmetics due to their high antioxidant properties, are in evergrowing demand currently. Since fungus natural resources are limited, the search for new highly productive Inonotus obliquus strains and the methods for its cultivation development with the high production ability of the main active component - melanin, are relevant. The aim of the present study was to isolate a new Inonotus obliquus strain from natural chaga, introduce it into a standard culture registered in an international database, as well as determine the optimal cultivation environment for the procurement of melanins with high antioxidant properties. A new strain of the fungus Inonotus obliquus SUB2092728 was isolated. For its growth, an agar nutrient medium was selected. It was shown that Inonotus obliquus SUB2092728 has a maximum growth rate of 2.18 mm/day in a glucose-potato medium with the onset of pigmentation on the 8th day of growth. The introduction of the hydrolysed lignin-containing drug Polyphepan into the environment made it possible to intensify the growth of the fungus and increase its growth rate up to 3.60 mm/day. It was established that the obtained melanins differ from the natural chaga melanin in terms of a lower degree of aromaticity and a higher degree of aliphaticity, as well as exhibiting antioxidant properties 16 % higher than natural melanin, making them suitable for use in the development of biologically active additives having antioxidant properties.

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