On aggregation in binary biopolymer systems
https://doi.org/10.21285/2227-2925-2020-10-2-223-231
Abstract
The main structural protein of the human eye, which accounts for about 50 % of the mass of all water-soluble proteins comprising the lens, is α-crystallin. Alpha-crystallin functions as a molecular chaperone, preventing other lens crystallins from interfering in the vital activity. Alpha-crystallins partially or fully stabilise unfolded proteins, preventing the formation of deposits, helping to preserve the lens transparency and reducing the risk of a number of diseases, including cataracts. This biological phenomenon can be considered in the framework of materials science when considering the problem of slowing down the aging processes of polymers. In the present study, methods for slowing down the process of aggregation of α-lactalbumin in solution are considered, using the binary system α-lactalbumin–αA-crystallin as an example. To this end, experimental data on the rate of change of the aggregation process were formalised, i.e. expressed in terms of transition temperatures and plasticisation functions of the components. The proposed expressions make it possible to clarify the concentration dependence of the initial aggregation rate, its order, and also to quantify the effect of the dose of UV irradiation on the aging process of the system. The experimentally obtained result means that an increase in the content of α-crystallin leads to an additional blocking of hydrogen bonds in the surface layers of α-lactalbumin and, accordingly, to an increase in the plasticising effect. In addition, the obtained expression of the activation energy of polymer chain rearrangement helps to account for the influence of infrared radiation on the development of so-called thermal cataracts (usually occurring in glassblowers, steelmakers, blacksmiths, welders, etc.), when the etiological factor consist in infrared rays having wavelengths from 0.74 to 2.50 microns, which freely pass through the cornea and iris without damaging them, and are largely adsorbed by the lens, causing its overheating.
About the Authors
Yu. I. Matveev
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
Russian Federation
Cand. Sci. (Physics and Mathematics), Senior Scientist,
4, Kosygin St., Moscow, 119334, Russian Federation
Russian Federation
Cand. Sci. (Physics and Mathematics), Senior Scientist,
4, Kosygin St., Moscow, 119334, Russian Federation
E. V. Averyanova
Biysk Technological Institute (branch) of the Altay State Technical University
Russian Federation
Cand. Sci. (Chemistry), Associate Professor, Department of Biotechnology
27, Geroi Sovetskogo Soyuza Trofimov St., Biysk, 659305, Russian Federation
Russian Federation
Cand. Sci. (Chemistry), Associate Professor, Department of Biotechnology
27, Geroi Sovetskogo Soyuza Trofimov St., Biysk, 659305, Russian Federation
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For citations:
Matveev Yu.I., Averyanova E.V. On aggregation in binary biopolymer systems. Proceedings of Universities. Applied Chemistry and Biotechnology. 2020;10(2):223-231. https://doi.org/10.21285/2227-2925-2020-10-2-223-231
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