Phytate hydrolysing activity of the Aspergillus niger L-4 micromycete strain
https://doi.org/10.21285/2227-2925-2020-10-2-232-239
Abstract
The aim of the study was to study the phytase synthesis capability of Aspergillus niger L-4 strain. The method for determining phytase activity is based on establishing the content of inorganic phosphates as a result of the action of phytase on the substrate under certain standard conditions by binding them with a vanadium-molybdenum reagent to form a coloured complex. The use of phytases for the hydrolysis of phytates in animal feed is important from the point of view of preserving the environment: when phytate complexes are destroyed, phosphorus is released, which performs an important structural and regulatory function, ensuring the normal development of bone and dental tissues and supporting their safety and integrity. Phosphoric acid is involved in the synthesis of kinases responsible for the normal course of chemical reactions in cells, in fat metabolism, as well as in the synthesis and breakdown of starch and glycogen. This reduces the release of undigested phosphorus into the environment. The object of the study consisted of native solutions obtained by culturing an industrial strain of acid-forming A. niger L-4 on various carbohydrate-containing media. The A. niger L-4 strain, previously selected at the All-Russian Scientific Research Institute of Food Additives for fermentation of molasses, has the ability to synthesise extracellular phytase. This paper presents the results of studies of phytase activity during the cultivation of A. niger L-4 on carbohydrate-containing media. It was found that in order components of the sucrose-mineral medium provide an elevated level of low-molecular-weight sugars necessary for increasing the productivity of phytase biosynthesis. Phytase activity in the native solution was shown to increase over 72 hours of fermentation to reach a value of 25.8±0.1 units/cm3. The phytase activity was 1.5 times higher than the fermentation process of a corn starch hydrolysate with a dextrose equivalent DE = 21±1 %, ensuring the productive biosynthesis of citric acid.
About the Authors
N. M. Musta Ogly
University of Information Technology, Mechanics and Optics
Russian Federation
Russian Federation
Postgraduate Student
9, Lomonosov St., St. Petersburg, 191002, Russian Federation
N. Yu. Sharova
University of Information Technology, Mechanics and Optics;
All-Russian Research Institute of Food Additives – a branch of the Federal Scientific Center named after V.M. Gorbatov
Russian Federation
Dr. Sci. (Engineering), Professor; Chief Researcher
9, Lomonosov St., St. Petersburg, 191002, Russian Federation;
55, Liteinyi Ave., St. Petersburg, 191014, Russian Federation
Russian Federation
Dr. Sci. (Engineering), Professor; Chief Researcher
9, Lomonosov St., St. Petersburg, 191002, Russian Federation;
55, Liteinyi Ave., St. Petersburg, 191014, Russian Federation
References
1. Fugthong А, Boonyapakron K, Sornlek W, Tanapongpipat S, Eurwilaichitr L, Pootanakit K. Biochemical characterization and in vitro digestibility assay of Eupenicillium parvum (BCC17694) phytase expressed in Pichia pastoris. Protein Expression and Purification. 2010;70(1):60–67. https://doi.org/10.1016/j.pep.2009.10.001
2. Hamad HO, Alma MH, Ismael HM, Göçeri A. The effect of some sugars on the growth of Aspergillus niger. Doga Bilimleri Dergisi = Journal of Natural Scienses. 2014;17(4):7–11. https://doi.org/10.18016/ksu-jns.28479
3. Greiner R, Da Silva LG, Couri S. Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9. Brazilian Journal of Microbiology. 2009;40(4):795–807. https://doi.org/10.1590/S1517-83822009000400010
4. Gargova S., Sariyska M. Effect of culture conditions on the biosynthesis of Aspergillus niger phytase and acid phosphatase. Enzyme and Microbial Technology. 2003;32(2):231–235. https://doi.org/10.1016/S0141-0229(02)00247-8
5. Da Silva LG, Trugo LC, Da Costa Terzi S, Couri S. Low phytate lupin flour based biomass obtained by fermentation with a mutant of Aspergillus niger. Process Biochemistry. 2005;40(2):951–954. https://doi.org/10.1016/j.procbio.2004.02.016
6. Casey A, Walsh G. Purification and characterization of extracellular phytase from Aspergillus niger ATCC 9142. Bioresource Technology. 2003;86(2):183–188. https://doi.org/10.1016/S0960-8524(02)00145-1
7. Ptak A, Bedford MR, Świątkiewicz S, Żyła K, Józefiak D. Phytase modulates ileal microbiota and enhances growth performance of the broiler chickens. PLoS One. 2015;10(3). 15 p. https://doi.org/10.1371/journal.pone.0119770
8. Vucenik I, Shamsuddin AKM. Cancer inhibition by inositol hexaphosphate (IP6) and inositol: from laboratory to clinic. Journal of Nutrition. 2003;133(11):3778S–3784S. https://doi.org/10.1093/jn/133.11.3778S
9. Coban HB, Demirci A. Screening of phytase producers and optimization of culture conditions for submerged fermentation. Bioprocess and Biosystems Engineering. 2014;37(4):609–616. https://doi.org/10.1007/s00449-013-1028-x
10. Jariwalla RJ. Rice-bran products: phytоnutrients with potential applications in preventive and clinical medicine. Drugs Experimental and Clinical Research. 2001;17(1):17–26.
11. Grases F, Simonet BM, Prieto RM, March JG. Variation of InsP4, InsP5 and InsP6 levels in tissues and biological fluids depending on dietary phytate. The Journal of Nutritional Biochemistry. 2001;12(10):595–601. https://doi.org/10.1016/S0955-2863(01)00178-4
12. Niu J, Arentshorst MP, Deepa SN, Dai Z, Baker SE, Frisvad JC, et al. Identification of a classical mutant in the industrial host Aspergillus niger by systems genetics: LaeA is required for citric acid production and regulates the formation of some secondary metabolites. G3: Genes, Genomes, Genetics. 2016;6(1):193–204. https://doi.org/10.1534/g3.115.024067
13. Shivanna GB, Venkateswaran G. Phytase production by Aspergillus niger CFR 335 and Aspergillus ficuum SGA 01 through submerged and solid-state fermentation. The Scientific World Journal. 2014;29. Article ID 392615, 6 p. https://doi.org/10.1155/2014/392615
14. Mitchinson C, Solheim LP. Method for liquefying starch. US patent, no. 5652127, 1997.
15. Kvist S., Carlsson T., Lawther J.M., DeCastro F.B. Process for the fractionation of ereal brans. US patent, no. 20050089602, 2005.
16. Sharova NYu. Producing the inhibitor of amylase during fermentation of starch with acidforming strain Aspergillus niger л-4. Vestnik Rossiiskoi akademii sel'skohozyajstvennyh nauk = Vestnik of the Russian agricultural science. 2013;3:45–47. (In Russian)
17. Sharova NYu, Safronova VI. Alfpfingerprinting for pasportization of Aspergillus niger L-4, the citric acid commercial producer. Sel'skohozyaistvennaya biologiya. 2016;51(2):204–212. (In Russian) https://doi.org/10.15389/agrobiology.2016.2.204rus
18. Sharova NYu, Pozdnyakova TA, Vybornova TV, Kulev DH. Method for producing citric acid, alpha-amylase and glucoamylase. Patent RF, no. 2366712; 2007. (In Russian)
19. Sharova NYu, Nikivorova TA. Regulation of an orientation in biosynthesis of the citric acid during bioconversion of starch hydrlysates with nould fungus Aspergillus iniger. Vestnik Rossiiskoi akademii sel'skohozyajstvennyh nauk = Vestnik of the Russian agricultural science. 2007;6:19–21. (In Russian)
20. Suleimenova Zh, Akhmetsadykov N, Kalieva A, Mustafin K, Saduyeva Zh. Effect of different cultural conditions for phytase production by Aspergillus niger in submerged fermentation. Advances in Enzyme Research. 2016;4(2):62–67. https://doi.org/10.4236/aer.2016.42007
Review
For citations:
Musta Ogly N.M., Sharova N.Yu. Phytate hydrolysing activity of the Aspergillus niger L-4 micromycete strain. Proceedings of Universities. Applied Chemistry and Biotechnology. 2020;10(2):232-239. https://doi.org/10.21285/2227-2925-2020-10-2-232-239
Views:
618
Email this article 