Formation of primary metabolites and chlorophyll in the plants of Cucumis sativus L. when exposed to l-ramnose conjugated with ma-aminobenzoic acid
https://doi.org/10.21285/2227-2925-2021-11-1-90-98
Abstract
This article studies the effect of L-rhamnose conjugated with m-aminobenzoic acid on the formation of primary metabolites and chlorophyll during germination of cucumber (Cucumis sativus L.) seeds. This synthetic aminoconjugate (rhamnosylamine) has an inhibitory effect on the growth of the plant under study, which weakens with a decrease in the solution concentration from 0.05 to 0.0005%. An analysis of changes in the FTIR spectra of the root and hypocotyl samples of germinated seeds showed significant changes in the carbohydrate pool and protein structure of the biomaterial. As the aminoconjugate content decreases to 0.0005%, the intensity of the bands at 1060, 1100 and 1158 cm-1 increases, indicating the accumulation of cellulose polysaccharides. The intensity of the amide bands changes in a similar way, while the values of the wave numbers of the second derivatives of the spectral signals in the 1600-1700 cm-1 range indicate conformational changes in proteins during germination. The electronic spectra of extracts and the vibrational spectra of cotyledon samples demonstrated a more intense formation of chlorophyll in the systems comprising the aminoconjugate; the chlorophyll content increases with a decrease in the content of the ami- noconjugate in the medium. These spectra additionally indicate a different state of the photosynthetic pigments in the control and experimental samples. According to our assumption, the effect of the aminoconjugate on the growth parameters, the formation of primary metabolites and photosynthetic pigments can be explained by its presence in the medium simultaneously with m-aminobenzoic acid and L-rhamnose. The latter substances are formed as the products of hydrolysis, the course of which was confirmed by polarimetric measurements. The obtained results confirm the prospects of searching and testing compounds containing structural components of different action.
About the Authors
I. S. Cherepanov
Udmurt State University
Russian Federation
Russian Federation
Igor S. Cherepanov, Cand. Sci. (Chemistry), Associate Professor
1, Universitetskaya St., Izhevsk, 426034
A. A. Zhuravleva
Udmurt State University
Russian Federation
Russian Federation
Alina A. Zhuravleva, Student
1, Universitetskaya St., Izhevsk, 426034
References
1. Ginak AI, Suleimankadiev SE, Suleimankadieva AE. Growth-regulating activity of 2-substituted thiazolidines on sunflower sprouts. Izvestiya Volgogradskogo gosudarstvennogo tekhnicheskogo universiteta = Herald of Volgograd State Technical Institute University. 2008;1:98–101. (In Russian)
2. Ol’shevskaya VA, Cherepanov IA, Spiridonov YuA, Spiridonova GS, Makarenkov AV, Samarskaya AS, et al. Herbicidal activity of carboranes, sydnone imine and ferrocene derivatives. Agrokhimiya. 2017;4:16–21 (In Russian)
3. Cherepanov IS. Spectral characteristics and biological activity of synthetic humic-like substances on the basis of carbohydrates. Journal of Physics: Conference Series. 2019;1399(5):055001. https://doi.org/10.1088/1742-6596/1399/5/055001
4. Butler HJ, McAinsh MR, Adams S, Martin FL. Application of vibrational spectroscopy techniques to non-destructively monitor plant health and development. Analitical Methods. 2015;7;4059–4070. https://doi.org/10.1039/c5ay00377f
5. Turker-Kaya S, Huck CW. A review of midinfrared and near-infrared imaging: principles, concepts and application in plant tissue analysis. Molecules. 2017;22(1):168. https://doi.org/10.3390/molecules22010168
6. Dmitrieva ED, Siundiukova KV, Akatova EV, Leont’eva MM, Volkova EM, Muzafarov EN. Biological activity of humic substances sapropel Upa river Tula region. Khimiya rastitel’nogo syr’ja = Chemistry of plant raw material. 2017;1:137–144 (In Russian) https://doi.org/10.14528/jcprm2017011418
7. Stewart D. Fourier transform infrared microspectroscopy of plant tissues. Applied Spectroscopy. 1996;50(3):357–365. https://doi.org/10.1366/0003702963906384
8. Buensanteai N, Sompong M, Saengchan C, Thumanu K. The cellular components of cucumber seedlings after primed with plant growth promoting rhizobacteria, Bacillus subtilis Bs008. African Journal of Microbiology Research. 2014;8(10):1006– 1011. https://doi.org/10.5897/AJMR12.1856
9. Wu J, Guo J, Hu Y, Gong H. Distinct physiological responses of tomato and cucumber plants in silicon-mediated alleviation of cadmium stress. Frontiers in Plant Science. 2015;6:453. https://doi.org/10.3389/fpls.2015.00453
10. Thumanu K, Sompong M, Phansak P, Nontapot K, Buensanteai N. Use of infrared microspectroscopy to determine leaf biochemical composition of cassava in response to Bacillus subtilis CaSUT007. Journal of Plant Interactions. 2015;10(1):270–279. https://doi.org/10.1080/17429145.2015.1059957
11. Rico CM, Peralta-Videa JR, Gardea-Torresday JL. Differential effect of cerium oxide nanoparticles on rice, wheat and barley root: a Fourier transform infrared (FT-IR) spectroscopy. Applied Spectroscopy. 2015;69(2):287–295. https://doi.org/10.1366/14-07495
12. Chu H-L, Lu T-Y, Lin S-Y. Effect of cyanide concentration on the secondary structures f protein in the crude homogenates of the fish gill tissue. Aquatic Toxicology. 2001;55(3–4):171–176. https://doi.org/10.1016/s0166-445x(01)00177-1
13. Yang J, Yen H. Early salt stress effect on the changes in chemical composition in leaves of Ice plant and Arabidopsis. A Fourier transform infrared spectroscopy study. Plant Physiology. 2002;130;1032– 1042.
14. Mouille G, Robin S, Lecomte M, Pagant S, Hofte H. Classification and identification of Arabidopsis cell wall mutants using Fourier-transform infrared (FTIR) microspectroscopy. The Plant Journal. 2003;35(3):393–404. https://doi.org/10.1046/j.1365-313X.2003.01807.x
15. Gorgulu ST, Dogan M, Severcan F. The characterization and differentiation of higher plants by Fourier transformed infrared spectroscopy. Applied Spectroscopy. 2007;61(3):300–308. https://doi.org/10.1366/000370207780220903
16. Chapados C, Lemieux S, Carpentier R. Protein and chlorophyll in photosystem II probed by infrared spectroscopy. Biophysical Chemistry. 1991; 39(3):225–239. https://doi.org/10.1016/0301-4622(91)80001-8
17. Antonov VI, Yagodin VI. Spectral characteristics of chlorophyll preparations from fit-tree wood greens. Khimiya rastitel’nogo syr’ja = Chemistry of plant raw material. 2006; 2:47–49 (In Russian)
18. Crisan M, Grozav M, Kurunczi L, Ilia G, Bertea CM. Inhibitory effect of some synthetic monoethanolamine salt of para-substituted benzoic acids and corresponding benzoic acids on cucumber seed germination. Journal of Plant Interactions. 2007;02:53–61. https://doi.org/10.1080/17429140701422496
19. Guikema J, Freeman L, Fleming EY. Effect of gabaculine on pigment biosynthesis in normal and nutrient deficient cell of Anacystis nidulans. Plant Physiology. 1986;82(1):280–284. https://doi.org/10.1104/pp.82.1.280
Review
For citations:
Cherepanov I.S., Zhuravleva A.A. Formation of primary metabolites and chlorophyll in the plants of Cucumis sativus L. when exposed to l-ramnose conjugated with ma-aminobenzoic acid. Proceedings of Universities. Applied Chemistry and Biotechnology. 2021;11(1):90-98. (In Russ.) https://doi.org/10.21285/2227-2925-2021-11-1-90-98
Views:
384
Email this article 