INVESTIGATION OF THE EFFECT OF ELECTROPORATION ON THE PRESERVATION OF VITALITY OF ASPERGILLUS NIGER MICROMYCETE - A PRODUCER OF CITRIC ACID
https://doi.org/ 10/21285/2227-2925-2017-7-3-54-59
Abstract
The influence of electrical impact on the preservation of the vitality of mycelial fungi on the example of Aspergillus niger micromy-cete was studied. The object of the study was the A. niger VKPM F-171 strain, an industrial citric acid producer, which was select-ed at the FGBNU VNIIPD. Electroporation of the fungus culture fluid was carried out during 72 hours after seeding of the nutrient medium using electroporators with generators of exponential and quadratic waves. Using a quadratic wave generator, the electroporation conditions of the culture liquid were selected and electroporation parameters established; this did not lead to any loss of vitality of the producer fungus. Using the chosen electroporation parameters, the mass concentration of organic acids in the culture liquid is increased by 12.1%. The data obtained can be used for future transformation of macromolecules into the A. niger cell by means of electroporation.
About the Authors
T. V. Vybornova
All-Russian Scientific-Research Institute of Food Additives
Russian Federation
Russian Federation
A. A. Kornev
St. Petersburg National Research Academic University of Russian Academy of Sciences
Russian Federation
Russian Federation
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For citations:
Vybornova T.V., Kornev A.A. INVESTIGATION OF THE EFFECT OF ELECTROPORATION ON THE PRESERVATION OF VITALITY OF ASPERGILLUS NIGER MICROMYCETE - A PRODUCER OF CITRIC ACID. Proceedings of Universities. Applied Chemistry and Biotechnology. 2017;7(3):54-59. (In Russ.) https://doi.org/ 10/21285/2227-2925-2017-7-3-54-59
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